Diabetic foot is a catastrophic complication of diabetes. This study included isolation and identification of three types of bacteria that cause diabetic foot ulcers,  fifty-five isolates of Staphylococcus aureus, thirty-five isolates of Acinetobacter baumannii, and thirty isolates of Serratia marcescens. These isolates were obtained from diabetic foot patients at different private clinics in and around Baghdad and Medical City Hospital. The proportion of male patients was greater than females, and it was noted that the age group (51-68 years) was more ages affected by diabetic foot. These isolates showed high resistance to most of the antibiotics used, Staphylococcus aureus was resistant to

This study aimed to detect of contamination of milk and local soft cheese with Staphylococcus aureus and their enterotoxins with attempt to detect the enterotoxin genes in some isolates of this bacteria. A total of 120 samples, 76 of raw milk and 44 of soft cheese were collected from different markets of Baghdad city. Enterotoxins in these samples were detected by VIDAS Set 2 system and it was found that enterotoxin A is present in a rate of 44.74% in milk samples and in a rate 54.50% in cheese samples. While other enterotoxins B, C, D, E were not found in any rate in any samples.
Through the study 60 isolates obtained from milk and cheeses were identified as Staphylococcus aureus by cultural, morphological and biochemical test by u

Thirty uropathogenic E. coli isolates were isolated from hospitalized and non hospitalized patients, complaining of urinary tract infections, of Al-Kadhymia Teaching Hospital and subjected to tRNA extraction. A method of tRNA extraction was modified by adding sodium dodecyl sulfate (SDS) instead of urea. Polyacrylamide gel electrophoresis and two methods of staining, ethidium bromide staining and silver staining, as well as spectrophotometric detection were used.

The purpose of this study to synthesize and characterize silver nanoparticles using phenolic compounds obtained from Camellia sinensis, to test the antibacterial properties of biosynthesized nanoparticles on the formation of biofilms in multidrug-resistant Pseudomonas aeruginosa. Ten isolates of P. aeruginosa were obtained from the Genetic Engineering and Biotechnology Institute laboratories of the University of Baghdad. By using the VITEK-2 system and culturing the isolates on cetrimide agar, the diagnosis was confirmed. Camellia sinensis silver nanoparticles (CAgNPs) were created using an extract of the plant's aqueous and methanolic leaves. Based on the results of the nanoparticle synthesis, spherical nanoparticles that may be single or

Lactobacillus is one of Lactic Acid Bacteria group, they are known to exhibit antagonistic activity against pathogenic organisms. This study evaluates the protective effect of Lactobacillus acidophilus Crud Bacteriocin like substance against growth of some food borne pathogenic bacteria. Antimicrobial effect of crud bacteriocin like substance was tested against the growth of S. typhi and S. aureus that isolated from food samples by agar well diffusion assay.
The results showed that the crud extract of bacteriocin like substance had antibacterial activity against the two tested bacteria, and the effect against S. typhi was greater than that of S. aureus , the zone of inhibition was (15)mm against S.&n

In accordance with epidemic COVID-19, the elevated infection rates, disinfectant overuse and antibiotic misuse what led to immune suppression in most of the population in addition to genotypic and phenotypic alterations in the microorganisms, so a great need to reevaluate the genetic determinants that responsible for bacterial community (biofilm) has been raised. A total of 250 clinical specimens were obtained from patients in Baghdad hospitals and streaked on Mannitol salt agar medium. The results revealed that 156 isolates appeared as round yellow colonies, indicating that they were mostly identified as Staphylococcus aureus from 250 specimens. The antibiotic resistance pattern of the isolates for methicillin 37.17% (n=58), Amoxic