سرطان البنكرياس هو مرض ذو معدل وفيات مرتفع، ولا يزال التشخيص المبكر لسرطان البنكرياس يمثل تحديًا. يظل معدل البقاء النسبي لمدة 5 سنوات أقل من 8%، والاستراتيجيات العلاجية غير فعالة في زيادة معدلات بقاء المريض على قيد الحياة. في خلايا سرطان البنكرياس، ارتبطت مقاومة العلاج بالتغيرات الجينية التي تؤدي إلى ظهور مسارات خلوية شاذة؛ ولذلك، هناك ما يبرر ايجاد استراتيجيات جديدة لعلاج هذا المرض. هنا، سعينا لاستكشاف مُنشط p53، APR-246، إما كدواء أحادي أو مدمج مع دوكسوروبيسين في خط خلايا AsPC-1 ذات بروتين p53 المتحول (p53-mut). تمت دراسة السمية الخلوية لـدوائي APR-246 ودوكوروبيسين اما منفردة أو مجتمعة في خط خلايا AsPC-1 مع اختبار MTT. تم حساب مؤشر معامل قياس التأزر (Combination Index) بناءً على نهج Chou و Talalay باستخدام برنامج CalcuSyn. تم تقييم موت الخلايا المبرمج بعد تصبيغها باستخداممادة الاكريدين اورنج/ايثيديوم برومايد (AO/EB). تم فحص التغيرات المورفولوجية تحت المجهر المقلوب بقوة تكبير 200 مرة تم قياس كمية التعبير الجيني باستخدام فحص النسخ العكسي الكمي PCR . أظهر APR-246 وحده تأثيرات متواضعة مضادة للتكاثر، في حين أظهر دوكسوروبيسين مع APR-246 تأثيرات تآزرية مرتبطة بالتغير المورفولوجي؛ لم يلاحظ أزدياد في نسبة موت الخلايا المبرمج في الخلايا المعاملة بكلا العقارين و هذا ما اكدته مستويات جين ال NOXA. في الختام، APR-246، سواء كان العلاج وحيدًا أو مقترنًا بـدوكسوروبيسين، كان يعيق تكاثر خلايا AsPC-1 ذات p53-mut، وهناك ما يبرر إجراء المزيد من الأبحاث في المختبر وفي الجسم الحي.
The Influence of Some Vitamins and Biochemical Parameters on Iraqi Females’ Patients with Malignant Breast Cancer"
Radon and its daughters are of the natural radioactive decay of the uranium series. Exposure to radon gas leads to lung cancer, so the risks are significantly higher for smokers than for non-smokers. Therefore, the risk of radon increases for both active and passive smokers. The radioactivity of alpha particles emitted by radium 226, the main source of radon 222, has become harmful because its prevalence and inhalation increase with increased smoking. In this study, a CR-39 detector was used to measure radon, radium, and uranium concentrations and then calculate risk parameters in seven cigarette-smoking females in vitro study of human blood samples, and three normal females with no actual and passive cigarette smoking. The
... Show MoreMedicinal plant life have performed an integral role in the development of human lifestyle being used as resources for the manufacturing of contemporary medicines, antibiotics. Microorganisms are responsible for many issues in industry and remedy because of biofilm formation. This study aimed to take a look at the results of Thymus vulgaris alcoholic extract on Staphylococcus aureus the causative marketers of acne in human particularly the women. The extract was acquired mixing 25 g of Thyme powder with 350 ml of 80% ethanol for 6 hours at 40°. Antimicrobial activities of the plant extract and integral oil towards the S. aureus were decided using the agar diffusion technique. The consequences proven that special concentrations of alcoholic
... Show MoreProstate cancer (PC), accounts for more than one-fourth of all cancer diagnoses, and the most frequently diagnosed cancer among men in 2022. The immunoglobulin (IG) Program death ligand-1(PD-1) cell surface receptor is predominantly expressed on the surface of many cells. The purpose of this study was to demonstrate the relationship between Program death ligand expression and some aggressive features of prostate cancer including perineural invasion, vascular invasion and necrosis. Thirty cases of prostate cancer with age range from 60 to 80 year old and 30 cases of normal prostate tissue with age under 25 year old were separated into two groups in a retrospective case-control
... Show MoreContracting cancer typically induces a state of terror among the individuals who are affected. Exploring how glucose excess, estrogen excess, and anxiety work together to affect the speed at which breast cancer cells multiply and the immune system’s response model is necessary to conceive of ways to stop the spread of cancer. This paper proposes a mathematical model to investigate the impact of psychological panic, glucose excess, and estrogen excess on the interaction of cancer and immunity. The proposed model is precisely described. The focus of the model’s dynamic analysis is to identify the potential equilibrium locations. According to the analysis, it is possible to establish four equilibrium positions. The stability analys
... Show MoreThis paper presents a new Azo dye that was prepared from the reaction of the Benzene-1,2-diamine and 1-(2,4,6-Trihydroxy-phenyl)-ethanone, Azo dye was used to prepare a new series of complexes with general formula: [Co2(H4L) Cl2(H2O)4] and [M2(H4L)Cl4(H2O)2] (M= Cr+3, Fe+3,Rh+3 and Ru+3). The prepared materials were different measurements including to infrared, ultraviolet-visible, and mass spectrometry, as well as thermo gravimetric analysis, differential calorimetry, and elemental analysis. Conductivity, magnetic susceptibility, metal content, and chlorine content of the complexes were also assessed. The complexes prepared from the dye were used to determine their ability to inhibit free radicals by measuring their antioxidant capacity us
... Show Moreالوصف Mixed ligand complexes of Cu (II), Co (II) and Zn (II) with 2-((4-(1-(4-chlorophenylimino) ethyl) phenylimino) methyl) phenol (L) and histidine (His) have been prepared and diagnosed by ¹H and13 C NMR, FT-IR and electronic spectral data, thermal gravimetric, molar conductance and metal analysis measurements. The ligand (L) shows a bidentate nature and the coordination occurs through N and O atoms of imine group and phenol group respectively whereas (His) behave as tridentate ligand, coordinating through the-NH2 group and carboxylate oxygen group and N atoms of imidazole ring. The analytical studies for three complexes have shown octahedral structure. The anticancer activity was screened against human cancer cell such Follicular
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