Pseudomonas aeruginosa has been identified as the main causative agent responsible for severe infections in burn patients worldwide. This study aimed to investigate the prevalence of the exoU/exoS genotype in P. aeruginosa isolates collected from burn wound infections in Iraq. From January to April 2023, a total of eighty isolates of P. aeruginosawere obtained from patients with burn wound infections in two Iraqi hospitals (Teaching Baghdad Hospital and AL-Yarmok Hospital).The isolates were first identified using biochemical tests and then verified using molecular techniques, specifically by targeting the 16S rRNA gene with specific primers. The exoU/exoS genotype was detected using conventional polymerase chain reaction (PCR) by specifically targeting two genes, exoS and exoU.The study found that burn wound infections contained four distinct genotypesof P. aeruginosa, and these genotypes exhibited substantial differences (P<0.001). The exoU-/exoS+ genotype exhibited a significantly higher prevalence of 75% (60 out of 80 isolates), whereas the exoU-/exoS- genotype had a comparatively lower prevalence of 5% (4 out of 80 isolates). At the same time, a lower percentage of isolates, 6.25 % (5 out of 80 isolates), was found to contain the exoU gene only (exoU+/exoS- genotype). Notably, 11 isolates (13.75 %) exhibited the presence of both exoS and exoU (exoU+/exoS+ genotype; a highly virulence isolate).bioinformatics analysis indicates that other bacterial species, such as Klebsiella pneumoniae, Enterobacter cloacae, and Acinetobacter baumannii, can produce ExoU toxin. The findingsprovide valuable insights into the genetic diversity of P. aeruginosa in burn wound infections, with the presence of highly virulent strains harbouring two genes (exoSand exoU gene).
That the strength of the community and its cohesion and integrity of its structure and morals and the prevailing relations and the strength of its institutions depends all on the numbers of a generation of students saturation of the culture of the nation and proud of its heritage and maintained their customs and traditions and voluntarily accept its laws. Students are the future leaders who are more than capable of giving, they represent class they are educated conscious backbone of the nation and the subject of their hopes, and their wealth and energies warehouse actors produced, and one of the key pillars in achieving developments in all areas of life.
Based on the above, the current research aims to identify:
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... Show MoreBackground: Intestinal infections are frequently occur among children with cancer who receive chemotherapy. On the other hand, diarrhea is especially common and severe among cancer patients that develop neutropenia, either due to the disease itself or due to the intensive chemotherapy. There are many causes of diarrhea among those patients, but intestinal infections still an important etiology among them.
Objectives: to study the frequency of diarrhea among neutropenic children, with its infectious etiologies, especially the bacterial, fungal and parasitic causes.
Type of the study:Cross-sectional study.
Methods: the study was done in the Oncology
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Background: Radial neck fractures in children account for 5 to 10% of all elbow fractures in children. They are extra-articular fractures of the radius proximal to the bicipital tuberosity. The physis is typically involved as a Salter-Harris I or II pattern. Alternatively, the fracture sometimes is extraphyseal, through the metaphysis. In children there is considerable potential for remodeling after these fractures. Up to 30° of radial head tilt and up to 3 mm of transverse displacement are acceptable. Many modalities of treatment are available regarding Surgical &Non-Surgical treatments. Objectives: To evaluate the functional outcome after surgical percutaneous joystick reduction therapy of severely angulated radial neck fracture i
... Show MoreBackground: Radial neck fractures in children account for 5 to 10% of all elbow fractures in children. They are extra-articular fractures of the radius proximal to the bicipital tuberosity. The physis is typically involved as a Salter-Harris I or II pattern. Alternatively, the fracture sometimes is extraphyseal, through the metaphysis. In children there is considerable potential for remodeling after these fractures. Up to 30° of radial head tilt and up to 3 mm of transverse displacement are acceptable. Many modalities of treatment are available regarding Surgical &Non-Surgical treatments. Objectives: To evaluate the functional outcome after surgical percutaneous joystick reduction therapy of severely angulated radial neck fracture i
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Twenty five samples out of sixty wound swabs taken from burn patients were identified as P. aeruginosabacteria by conventional methods. Antibiotics susceptibility tests were performed against thirteen antibiotics. P. aeruginosa samples were treated with 0.5 mg/ml of Safranin O solution then irradiated with 532nm Q-switched Nd:YAG laser at four energy densities (0.324, 0.704, 1.380, and 1.831 J/cm2) for different times of 5, 8 and 11 minutes with 5Hz repetition rate. The viability, susceptibility to antibiotic and production of pyocyanin were determined before and after irradiation. The results showed that the number of CFU/ml of P. aeruginosa decreased with increasing the dose of irradiation. Complete killing of cells was observed at 1.8
... Show MoreResults: The results summarized two goals, the first goal stipulates (to identify the degree of cyberbullying among academically outstanding students in the middle school stage). To achieve this goal, the researchers applied the research tool (electronic bullying scale) and then extracted the arithmetic mean for the sample of the current research, which amounted to (6.28) with a standard deviation of (4.03). Then the researchers applied the t-test for one sample to identify the significance of the differences between the means. The arithmetic mean for the sample and the hypothetical (theoretical) mean, which amounted to (11.5) degrees, and after applying the T-test for one sample, it was found that the calculated T-value, which amounted to
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