STAG3 is the meiotic component of cohesin and a member of the Cancer Testis Antigen (CTA) family. This gene has been found to be overexpressed in many types of cancer, and recently, its variants have been implicated in other disorders and many human diseases. Therefore, this study aimed to analyze the major variants of STAG3. Western blot (WB) and immunoprecipitation (IP) assays were performed using two different anti-STAG3 antibodies that targeted the relevant protein in MCF-7, T-47D, MDA-MB-468, and MDA-MB-231 breast cancer cells with Jurkat and MCF-10A cells as positive and negative controls, respectively. In silico analyses were searched to study the major isoforms. WB and IP assays revealed two abundant polypeptides < 191 kDa and

Pre-eclampsia complicates 2-8% of all pregnancies and it is one of the leading causes of maternal mortality and pre-term delivery in the world. Unfortunately, there is scarcity of documents discussing the circulating level of several essential trace elements in Pre-eclampsia patients in Baghdad especially in the last trimester of the pregnancy. The present study was designed to quantitative evaluation the whole blood concentration of two trace elements, copper (Cu), and iron(Fe), in pre-eclamptic women in the third trimester of pregnancy. The study was conducted on 18 Pre-eclamptic pregnant women as patients group with clinical detected high blood pressure ≥140/90 mmHg and 13 normotensive pregnant women as control group from Al-Alwiya

This study includes isolation, purification, and identification of Fusarium oxysporum from chili pepper infected plants. Eucalyptus camaldulensis were collected and air dried at room temperature, then ground to semi powdered state. Phenols, alkaloids and terpens were extracted from Eucalyptus camaldulensis. The antifungal activity, type of extracts was evaluated at different concentrations 5 and 10 mg / ml of these compounds were prepared and their antagonistic activity was studied. The Percentage of radial growth inhibition of fungi by plant extracts was measured after 7 day incubation. Results showed that terpens extract was the most active against fungi and alkaloids extract had less antifungal activity and the percentage of mycelia r

Objectives: Severe head injury is the most devastating neurosurgical condition and it is only next to cancers as the leading cause of death in developed countries.
Because trace elements (TEs) are involved in most of enzymes that drives the biochemical reactions, so they are considered as a window to the biochemical
environment of the body in general and in brain in specific. 
Aim of the Study: This study measured six TEs (Fe, Zn, Mg, Cu, Mn and Co) in 29 patients with severe head injury (GCS Score 3-9); their ages between 5-50 years.
Collection and estimation performed at both Neurosurgical Hospital (NH) in Baghdad and Medical Research Center (MRC) of College of Medicine, Kadhimiyah between Janu

This study was aimed to investigate the iron overload effect on the levels of ferritin, testosterone, follicle stimulating hormone (FSH), luteinizing hormone (LH) and prolactin (PRL) in male patients with β-thalassemia. Blood levels of sex hormones and ferritin were determined in (50 males) beta-thalassemia patients, aged (16-23) years and in (30) healthy males matched for age. They were recruited from the Abin Al-Balady Hospital in Baghdad. Ichroma™ kits were used to determine the blood levels of sex hormones and ferritin. The results showed that the level of testosterone, LH and FSH were significantly (p≤ 0.05) lower in β-thalassemia male patients compared to controls. Furthermore, the level of ferritin and prolactin in male pati

Many people take protein supplements in an effort to gain muscle. However, there is some controversy as to whether this is really effective. There is evidence suggesting that consuming high level s of protein may in fact have negative side effects for health. The current study included 29 young Iraqi building muscles in two different groups (taken and not protein supplements) (age range=17-31 years), the cases were selected from family, friends, college students, and Gyms), from November 2014 to March 2015. A careful history was obtained from each volunteer including age, duration of sports, type of supplements, and family history of diseases. Some biochemical parameters like (glucose, urea, uric acid, creatinine, bilirubin, serum protei

    This study was aimed to investigate the role of crud alcoholic extract of Lallemantia royleana seeds in reducing the hepatotoxicity and side effect of rifadin drug in liver.                      The animals (40 mice) were divided into four groups, the first group was treated with normal saline (0.9%) for 28 days as a control and the second group was treated with rifadin (1.5 mg/kg/day) for 28 days and third group was treated with acoholic extract of Lallemantia royleana seeds (1% w/v) for 28 days, while the forth group was treated with alcoholic extract of seeds alone for 5 days and with alcoholic extract and rifadin for 28 days, so the total period of this group is 33