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Cytotoxic Activities, Determining Toxin, and Molecular Docking of Ovary Pufferfish (Tetraodon leiurus) in Singkarak Lake as Cancer Chemoprevention Candidate

The primary toxin class discovered in freshwater pufferfish is a category of neurotoxins called PSTs (Paralytic shellfish toxins) and pufferfish toxin has been observed to have biological, biochemical, and cytotoxic effects on cancer cell lines. Therefore, it is crucial to determine the cytotoxic activity, toxins present in the ovary of T. leiurus, and interaction between ligand (toxin compound) and receptors test. This study used the MTT method in the T47D cell lines, liquid chromatograph-tandem mass spectrometry (LC-MS/MS), and analysis of the molecular interaction using molecular docking. The ovary of T. leiurus had cytotoxicity on the T47D cell, having an IC50 value of 229.535 μg/ml, and generated a chromatogram with a retention duration of 1.25 min that was similar to the Decarbamoylneosaxitoxin (dcNEO) standard solution. In molecular interactions between the dcNEO ligand to receptors, the lowest ΔG value was -9.29 kcal/mol at the Nav 1.7 receptor, and the lowest KI value was 1.23 µM at the Mcl-1 receptor. These findings indicate that the ovary of T. leiurus is cytotoxic to the T47D cell line and contains dcNEO toxin. It is more stable for the dcNEO ligand to engage with the Nav 1.7 receptor than with other receptors, and it inhibits the Mcl-1 receptor more potently than with other receptors. These findings indicate that the ovary of T. leiurus may be chemotherapy for the prevention of cancer strategy.

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Publication Date
Fri Oct 04 2024
Journal Name
Chemical Review And Letters
Publication Date
Tue Jan 29 2019
Journal Name
Journal Of Accounting And Financial Studies ( Jafs )
The Impact of Tax Laws and Decisions in Determining the Tax Base of the Results of the Financial Statements of Companies

This research aims to measure the discrepancy between the accounting income which is prepared according to generally accepted accounting principles and the tax income that is being prepared according to the rules and tax laws, and find out the most important differences that arise between incomes.The research found the most important to the following conclusions:1.Faces determining the tax base for companies subject to income challenges related to the weakness of the efficiency and the possibility of technical angel tax and its ability to examine the financial statements submitted to the tax administration tax.2.That the tax system in Iraq does not comply with accepted accounting principles generally accepted.The research recommends the

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Publication Date
Wed Jul 01 2015
Journal Name
Journal Of The Faculty Of Medicine Baghdad
The role of Wilm’s Tumor1 immunohistochemical marker in surface epithelial ovarian tumors

Background: Wilms’ tumor 1 is a tumor suppressor gene. The gene is located in chromosome 11p13. And its expression was found in many solid tumors (including ovarian tumor) and also expressed in hematologic malignancies, Recent studies found that WT1 to be involved in angiogenesis.
Objectives: To evaluate the expression of WT1 in surface epithelial ovarian tumorand study the possibility of using WT1 as replacement of both;ovarian tumor marker CA125 and a endothelial cell phenotypic marker CD34.
Patients and methods: This is a study of a retrospective ( cross sectional ) of sixty cases with total abdominal hysterectomy and bilateral salpingo - oopherectomy collected from department of Histopathology – Teaching Laboratories / Medi

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Publication Date
Tue Mar 24 2020
Journal Name
Mintage Journal Of Pharmaceutical & Medical Sciences
Publication Date
Sat Jan 01 2022
Journal Name
Medico-legal Update
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Publication Date
Sun Jan 01 2023
Journal Name
Materials Today: Proceedings
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Publication Date
Sun Jan 01 2023
Journal Name
Materials Today: Proceedings
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Publication Date
Mon Jul 01 2013
Journal Name
Journal Of The Faculty Of Medicine Baghdad
The Pro-inflammatory IL6 and serum glucose in Polycystic Ovary Syndrome

Background: Polycystic ovary syndrome (PCOS) is one of the most common female endocrine disorders. PCOS produces symptoms in approximately 5% to 10% of women of reproductive age (12–45 years old).
Objective: To detect the level of IL-6 in PCOS patients correlated with their serum glucose.
Patients and methods: This is a prospective study carried out at Baghdad teaching hospital in Baghdad/Iraq from November 2011 to January 2012. In this study, 80 blood samples (40 untreated women with PCOS cases and 40 healthy control) were collected to investigate their IL-6 levels by using Enzyme linked immunosorbent assay (ELISA) technique and fasting blood sugar (FBS) by enzymatic assay.
Results: Serum IL-6 elevated in PCOS patients mainly

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Publication Date
Tue Sep 01 2020
Journal Name
Baghdad Science Journal
Identification of Acinetobacter baumannii and Determination of MDR and XDR Strains

The current study focuses on the bacterium Acinetobacter baumannii due to its importance as a nosocomial infections source in addition to its increased resistance against antibiotics. Different clinical and hospital environment samples were collected, and cultured on A. baumannii selective media: Leed Acinetobacter agar and Herellea agar. A. baumannii have been identified by traditional methods, followed by confirmation using molecular identification to detect blaoxa-51 like gene which is considered a diagnostic gene since it is present in genome of all A. baumannii strains. The result was, nineteen bacterial isolates of A.baumannii were obtained, from twenty-seven suspected isolate

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Publication Date
Sun Apr 05 2015
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Cancer in infancy: Experience of Children Welfare Teaching Hospital, Medical City Complex, Baghdad

Background: It is becoming increasingly apparent that the study of infant cancer may lead to further understanding of the mechanisms of carcinogenesis, due to the unique clinical, genetic, and epidemiologic characteristics of cancer in infant.
Objectives: To study the clinical and pathological presentation of infants with cancer, their outcome and survival.
Patients & methods: A retrospective study was done over a 6 years period between (Jan.1st.2001- Dec. 31st. 2006), reviewed the records of 85 infants diagnosed and treated in oncology unit, children welfare teaching hospital, medical city-Baghdad.
Results: The majority of patients 57(67%) were from Baghdad & surrounding provinces; Leukemia was the most common type of c

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