Bovine theileriosis, caused by Theileria annulate, is disease affecting cattle and buffaloes worldwide. The current study aimed to screen the blood samples of 30 naturally suspected local buffaloes infected with Theileria species. The blood samples were initially examined by light microscopic and then the positive samples were subjected to PCR reactions. All 30 animals indicated clinical symptoms, such as high fever, loss of appetite, the presence of the hard tick, and enlargement of lymph nodes. The amplified products of 18S rRNA were analyzed, along with molecular detection of the drug-binding site alterations and interrelated changes in the cytochrome b (cyto b) gene. Blood smears revealed the presence of infected erythrocytes wi

Background: Pumpkin seeds are a valuable source of high-quality protein and can be utilized as functional food ingredients due to their properties, such as solubility, foam formation, and stability. This study aims to produce protein isolate and its enzymatic hydrolysates from local pumpkin seeds to study their properties. Methodology: Preparing defatted pumpkin seeds for protein extraction, followed by the enzymes’ hydrolysis using Trypsin and Pepsin enzymes separately and together in two methods. The determination of amino acids and the degree of hydrolysis was conducted; moreover, protein properties were studied, including solubility, emulsifying activity, stability index, foaming capacity, and stability. Results: A protein sample was

The presence of hydrocarbons in the soil is considered one of the main problems of pollution. In our current study, eight samples isolated from soil saturated with hydrocarbons were taken from different areas of Baghdad, Iraq. In this study, 5 isolates belonging to Pseudomonas aeruginosa by 99%, 4 isolates to Klebsiella pneumoniae by 98%, and 3 isolates to Enterobacter hormaechei by 97% were diagnosed in different ways. A molecular examination was also conducted by 16sRNA. We recorded P. aeruginosa, K. Pneumoniae and E. hormaechei as new local isolates in NCBI. In addition, a comparison was made between our isolates and the global isolates to determine the degree of convergence in the evolutionary line. The genes alkB and nahAc7 were diagno

Background: Globally, breast cancer is the second leading cause of death among women in Iraq. Several genetic and environmental factors are associated..

Recent studies highlighted the hazard of emerging antimicrobial resistance (AMR) bacteria in domesticated animals, with multidrug resistance (MRD) patterns in poultry recorded with alarming intensity as a major reservoir. This resistance has been associated with the misuse and overuse of antimicrobial agents in poultry farms. This current study aimed to determine the multidrug resistance (MDR) patterns of Escherichia coli isolated from broiler chickens. A total of 32 human samples were taken from worker handlers (positive: 4 samples, 12.5%), and 40 samples were collected from poultry chickens (positive: 13 samples, 32.5%). The findings revealed marked resistance against six of the ten agents in the isolates (MDR being 100% in all samples an

Quercetin, a crucial plant flavonoid, exhibits a range of pharmacological properties. A significant body of literature explores the antibacterial activity of the substance and its potential mode of action. Quercetin has shown the capacity to inhibit the growth of several types of gram-positive and gram-negative bacteria, as well as fungi and viruses.