Acinetobacter baumannii (A. baumannii ) is considered a critical healthcare problem for patients in intensive care units due to its high ability to be multidrug-resistant to most commercially available antibiotics. The aim of this study is to develop a colorimetric assay to quantitatively detect the target DNA of A. baumannii based on unmodified gold nanoparticles (AuNPs) from different clinical samples (burns, surgical wounds, sputum, blood and urine). A total of thirty-six A. baumannii clinical isolates were collected from five Iraqi hospitals in Erbil and Mosul provinces within the period from September 2020 to January 2021. Bacterial isolation and biochemical identification of isolates were carried out followed by DNA extraction from 36 isolates and six negative ATCC strains (Salmonalle typhi, Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Enterobacter aeruginosa, Staphylococcus aures) and only one positive control ATCC A. baumannii using Phenol/Chloroform method. AuNPs were synthesized using the citrate reduction method and examined by XDR, FTIR, UV-VIS, FE-SEM, and TEM. The optimized colorimetric assay was employed based on unmodified spherical AuNPs and PCR amplification of 16S rRNA intergenic spacer sequences (ITS) with species-specific DNA oligo-targeters. Detection and optimization of A. baumannii amplicons using unmodified AuNPs were performed based on species-specific DNA oligonucleotide. The AuNPs assay was able to colorimetrically detect and distinguish A. baumannii from other ATCC bacterial isolates. The turnaround time of this assay was about 3 hours, including sample preparation and amplification, to show (0.025-6 ngµl-1) as a detection limit of DNA concentration. The efficacy of colorimetric detection was proved to effectively diagnose A. baumannii isolates with high sensitivity, simplicity, and robustness to rapidly diagnose A. baumannii isolates from different clinical samples.
English ابحث عن مقالات و مجلات ... تحليل علاقة ثقافة المنظمة بلازمة من منظور الفردبة - الجماعية أ.د زكـريا مطلك الدوري، أ.د سـعـد علي العـنزي، أ. يعرب عدنان السعيدي مجلة كلية بغداد للعلوم الاقتصادية الجامعة 2009, المجلد 2, العدد 20, الصفحات 107-122 الخلاصة تتعدد وجهات النظر بشأن الاسباب والمعالجات الممكنة للازمات، وان هذا الاختلاف يرجع بالاصل إلى تنوع المداخل التي يعتمد عليها الباحثين في تناول الظاهرة أو الموضوع. وضمن هذا
... Show Moreالحمد لله خالق الأكوان، وبارئ الإنسان، عظيم الشأن والإحسان، والصلاة والسلام على خاتم الأنبياء والمرسلين الصادق الأمين محمد وعلى آله الطيبين الطاهرين وعلى من سار على هديه بإحسان إلى يوم الدين.
أما بعد ...
فالقرآن الكريم هو ذروة الفصاحة والإعجاز والسلامة اللغوية يمثل بقراءاتهِ القرآنية المتعددة: المتواترة والشاذة والآحاد أصلا أصيلا للنحو العربي يحتج به في إقامة
... Show Moreشهدت معظم المدن السومرية أزدهاراً أقتصادياً خلال عصر أور الثالثة ،أنعكس ذلك من خلال النصوص المسمارية المكتشفة ذات المضامين الاقتصادية المتنوعة ومنها (وصولات تسلم Bullae) التي تميزت بعصر أور الثالثة بأشكالها المتعددة ومنها: الكروية والمربعة والمستطيلة والمثلثة والمضلعة ومعظمها يحتوي على طبعات أختام فضلاً عن أنها تكون مثقوبة طولياً لغرض التعليق.
Islamic civilization derived its religious law from Quran and sunnah which took care of human as a benefit person in life and how it treat with it , and because the civilization takes care of human , it would take also in his health , manners ,body and this is the aim of Islamic civilization ,all religious emphasize on Islamic laws and on helping poor people or weakness one.
ملامح من فكرة القانون الطبيعي في حضارة العراق القديم
Still Financial institutions, including banks, a key target for money launderers to transfer illicit funds to the legitimate funds and by weaknesses in the internal audit procedures applied in the banks or through a lack of legal structure to combat this phenomenon in addition to the procedures by other regulations
T
... Show Moreهدف الدراسة معرفة التأثير السمي التراكمي للأعلاف الملوثة بالحنطة المتفحمة على أفراخ فروج اللحم .اجري هذا البحث في مختبرات الصحة الحيوانية التابعة لوزارة العلوم والتكنولوجيا استعمل 78 فرخاً لهذه التجربة قسمت إلى ستة مجاميع ربيت في أقفاص خاصة أعدت لهذا الغرض. تم تغذيتها يوميا على أعلاف ملوثة حسب برامج التغذية المتبعة , تمثلت المجموعة الأولى بمجموعة السيطرة والتي غذيت على عليقه تحوي الحنطة
That grammatical studies, a phenomenon of the Arabic language,
enabling the researcher during his research of the development of sentences,
textual analysis, to be the result of that meaning semantic of a sentence, and
the statement of the function the individual, in terms of its meaning in
context, and a link to the meaning lexical give indications of the current
single, and correlation analysis II , in terms of significance with the current
status syntactic to that individual, and these of the most important features of
grammatical studies of modern, based on the relationship structure sense, and
the relationship of the meaning of the structure, we looked for Aiklo for such
a study, Koran, all meanings and co
The subject of the Internet of Things is very important, especially at present, which is why it has attracted the attention of researchers and scientists due to its importance in human life. Through it, a person can do several things easily, accurately, and in an organized manner. The research addressed important topics, the most important of which are the concept of the Internet of Things, the history of its emergence and development, the reasons for its interest and importance, and its most prominent advantages and characteristics. The research sheds light on the structure of the Internet of Things, its structural components, and its most important components. The research dealt with the most important search engines in the Intern
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