Preferred Language
Articles
/
bsj-6842
A Developed Colorimetric Assay Using Unmodified Gold Nanoparticles for the Identification of Acinetobacter baumannii Isolates from Different Clinical Samples
...Show More Authors

  Acinetobacter baumannii (A. baumannii ) is considered a critical healthcare problem for patients in intensive care units due to its high ability to be multidrug-resistant to most commercially available antibiotics. The aim of this study is to develop a colorimetric assay to quantitatively detect the target DNA of A. baumannii based on unmodified gold nanoparticles (AuNPs) from different clinical samples (burns, surgical wounds, sputum, blood and urine). A total of thirty-six A. baumannii clinical isolates were collected from five Iraqi hospitals in Erbil and Mosul provinces within the period from September 2020 to January 2021. Bacterial isolation and biochemical identification of isolates were carried out followed by DNA extraction from 36 isolates and six negative ATCC strains (Salmonalle typhi, Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Enterobacter aeruginosa, Staphylococcus aures) and only one positive control ATCC A. baumannii using Phenol/Chloroform method. AuNPs were synthesized using the citrate reduction method and examined by XDR, FTIR, UV-VIS, FE-SEM, and TEM.  The optimized colorimetric assay was employed based on unmodified spherical AuNPs and PCR amplification of 16S rRNA intergenic spacer sequences (ITS) with species-specific DNA oligo-targeters. Detection and optimization of A. baumannii amplicons using unmodified AuNPs were performed based on species-specific DNA oligonucleotide. The AuNPs assay was able to colorimetrically detect and distinguish A. baumannii from other ATCC bacterial isolates. The turnaround time of this assay was about 3 hours, including sample preparation and amplification, to show (0.025-6 ngµl-1) as a detection limit of DNA concentration. The efficacy of colorimetric detection was proved to effectively diagnose A. baumannii isolates with high sensitivity, simplicity, and robustness to rapidly diagnose A. baumannii isolates from different clinical samples.

Scopus Crossref
View Publication Preview PDF
Quick Preview PDF
Publication Date
Sun Jan 01 2017
Journal Name
Iec2017 Proceedings Book
Improving TF-IDF with Singular Value Decomposition (SVD) for Feature Extraction on Twitter
...Show More Authors

View Publication
Crossref (5)
Crossref
Publication Date
Fri Nov 01 2024
Journal Name
Optical Materials
Nanostructured LNTO saturable absorber for generating multi-wavelength laser in Q-switched EDFL
...Show More Authors

In this paper, we propose a new and efficient ferroelectric nanostructure metal oxide lithium niobate [(Li1.075Nb0.625Ti0.45O3), (LNTO)] solid film as a saturable absorber (SA) for modulating passive Q-switched erbium-doped fiber laser (EDFL). The SA is fabricated as a nanocomposite solid film by the drop-casting process in which the LNTO is planted within polyvinylidene fluoride-trifluoroethylene [P(VDF-TrFE)] as host copolymer. The optical and physical characteristics of the solid film are experimentally established. The SA is incorporated within the cavity of EDFL to examine its capability for producing multi-wavelength laser. The experimental results proved that a multi-wavelength laser is produced, where stable four lines with central

... Show More
View Publication
Scopus (14)
Crossref (12)
Scopus Clarivate Crossref
Publication Date
Tue Jul 01 2025
Journal Name
South African Journal Of Chemical Engineering
Expired azithromycin and cephalexin as corrosion inhibitors for aluminum in saline-acidic solution
...Show More Authors

View Publication
Scopus (3)
Crossref (1)
Scopus Clarivate Crossref
Publication Date
Fri Jan 01 2016
Journal Name
Journal Of Cosmetics, Dermatological Sciences And Applications
Tuberculin as Intralesional Therapy for Viral Warts—Single-Blind, Split, Placebo, Controlled Study
...Show More Authors

View Publication
Crossref (4)
Crossref
Publication Date
Mon Jan 01 2024
Journal Name
Journal Of Image And Graphics
Normalized-UNet Segmentation for COVID-19 Utilizing an Encoder-Decoder Connection Layer Block
...Show More Authors

The COVID-19 pandemic has had a huge influence on human lives all around the world. The virus spread quickly and impacted millions of individuals, resulting in a large number of hospitalizations and fatalities. The pandemic has also impacted economics, education, and social connections, among other aspects of life. Coronavirus-generated Computed Tomography (CT) scans have Regions of Interest (ROIs). The use of a modified U-Net model structure to categorize the region of interest at the pixel level is a promising strategy that may increase the accuracy of detecting COVID-19-associated anomalies in CT images. The suggested method seeks to detect and isolate ROIs in CT scans that show the existence of ground-glass opacity, which is fre

... Show More
View Publication
Scopus (5)
Crossref (1)
Scopus Crossref
Publication Date
Wed Oct 09 2024
Journal Name
Engineering, Technology & Applied Science Research
Improving Pre-trained CNN-LSTM Models for Image Captioning with Hyper-Parameter Optimization
...Show More Authors

The issue of image captioning, which comprises automatic text generation to understand an image’s visual information, has become feasible with the developments in object recognition and image classification. Deep learning has received much interest from the scientific community and can be very useful in real-world applications. The proposed image captioning approach involves the use of Convolution Neural Network (CNN) pre-trained models combined with Long Short Term Memory (LSTM) to generate image captions. The process includes two stages. The first stage entails training the CNN-LSTM models using baseline hyper-parameters and the second stage encompasses training CNN-LSTM models by optimizing and adjusting the hyper-parameters of

... Show More
View Publication
Scopus (6)
Crossref (5)
Scopus Crossref
Publication Date
Tue Oct 15 2019
Journal Name
International Journal Of Electrical And Computer Engineering (ijece)
Combining Convolutional Neural Networks and Slantlet Transform For An Effective Image Retrieval Scheme
...Show More Authors

In the latest years there has been a profound evolution in computer science and technology, which incorporated several fields. Under this evolution, Content Base Image Retrieval (CBIR) is among the image processing field. There are several image retrieval methods that can easily extract feature as a result of the image retrieval methods’ progresses. To the researchers, finding resourceful image retrieval devices has therefore become an extensive area of concern. Image retrieval technique refers to a system used to search and retrieve images from digital images’ huge database. In this paper, the author focuses on recommendation of a fresh method for retrieving image. For multi presentation of image in Convolutional Neural Network (CNN),

... Show More
Scopus (12)
Crossref (5)
Scopus Crossref
Publication Date
Wed Dec 01 2021
Journal Name
Baghdad Science Journal
New Common Fixed Points for Total Asymptotically Nonexpansive Mapping in CAT(0) Space
...Show More Authors

      Strong and ∆-convergence for a two-step iteration process utilizing asymptotically nonexpansive and total asymptotically nonexpansive noneslf mappings in the CAT(0) spaces have been studied. As well, several strong convergence theorems under semi-compact and condition (M) have been proved. Our results improve and extend numerous familiar results from the existing literature.

View Publication Preview PDF
Scopus (8)
Crossref (1)
Scopus Clarivate Crossref
Publication Date
Wed Feb 01 2017
Journal Name
Journal Of Controlled Release
Surface engineering tumor cells with adjuvant-loaded particles for use as cancer vaccines
...Show More Authors

View Publication
Scopus (34)
Crossref (33)
Scopus Clarivate Crossref
Publication Date
Fri Jun 30 2023
Journal Name
Iraqi National Journal Of Nursing Specialties
Instruction Program for Patient with Peptic Ulcer about Nutritional Habits: case-control study
...Show More Authors

Objectives: The purpose of the study is to ascertain the relationship between the training program and the socio-demographic features of patients with peptic ulcers in order to assess the efficiency of the program on patients' nutritional habits.

Methodology: Between January 17 and October 30 of 2022, The Center of Gastrointestinal Medicine and Surgery at Al-Diwanyiah Teaching Hospital conducted "a quasi-experimental study". A non-probability sample of 30 patients for the case group and 30 patients for the control group was selected based on the study's criteria. The study instrument was divided into 4 sections: the first portion contained 7 questions about demographic information, the second sect

... Show More
View Publication Preview PDF
Crossref (1)
Crossref