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Mesenchymal and stemness transdifferentiation via in-vitro infection of T24 cell line with Klebsiella pneumoniae

Klebsiella pneumoniae has been found in the urinary tract of some bladder cancer patients. Bacterial presence within tumor tissue may affect the tumor-microenvironment and consequently influence cancer behavior, development, and treatment response. This study investigated mesenchymal and stemness transdifferentiation of bladder cancer cell line due to environmental stress of K. pneumoniae. Cultures of urothelial bladder cancer cell line (T24) were infected with K. pneumoniae with different multiplicity of infection (MOI) for two and four days. Transdifferentiation-associated features were morphologically assessed.

Moreover, transdifferentiation markers were estimated using Q-PCR and immunohistochemistry. Q-PCR data showed an increase in mesenchymal transdifferentiation traits; vimentin expression was upregulated, and cytokeratin19 expression downregulated significantly (P<0.001) compared with controls, which were emphasized by immunohistochemistry results. Moreover, stemness transdifferentiation markers expression increased significantly (P<0.001). The heterogeneous tumor cell population may be altered by bacterial infection, which improves cancer cells' migration and self-renewal ability. Thus, bacteria may be engaged in cancer progression and metastases.  

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Publication Date
Sat Dec 30 2023
Journal Name
Iraqi Journal Of Science
Evaluation of Chlamydia Pneumoniae IgG in a Sample of Rheumatoid Arthritis Patients

Thirty serum samples of patients suffering from rheumatoid arthritis after screening of rheumatoid factor, C-reactive protein and ESR were collected and including in present study to detect the IgG antibody against Chlamydia pneumoniae by ELISA test. The results showed only 2(6%) patients had seropositive of C. pneumoniae, this lead to suggest that C. pneumoniae may be one of the etiological or trigger factor in patients of rheumatoid arthritis.

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Publication Date
Sun Oct 01 2023
Journal Name
Journal Of The Faculty Of Medicine Baghdad
A Cross-Sectional Study of Urinary Neutrophil Gelatinase-Associated Lipocalin and its Association with Steroid Responsiveness in Iraqi Children with Idiopathic Nephrotic Syndrome

Background: Steroid-resistant nephrotic syndrome (SRNS)is associated with serious complications and financial burdens. Studies reported increased urinary neutrophil gelatinase-associated lipocalin (uNGAL) levels in children with idiopathic nephrotic syndrome (INS).

Objectives: This study aimed to evaluate the uNGAL potential to distinguish SRNS from steroid-sensitive nephrotic syndrome (SSNS) in Iraqi children.

Patients and Methods: Children with SRNS (n=31) and SSNS (n=32) were recruited from Babylon Hospital for Maternity and Pediatrics from March to June 2022. Patients' data included demographics, clinical characteristics, and urinary lab tests. The uNGAL concentrations

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Publication Date
Mon Oct 01 2012
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Determination of Cancer Antigen CA15-3 and Carcino Embryonic Antigen CEA concentration as Tumor Markers in Patients with Stomach and Colorectal cancers.

Background: Tumor markers are often requested as part of a diagnostic workup, and increased concentrations in serum may suggest malignancy of a particular organ. However, definitive diagnosis is based on histological evaluation of the involved tissue.
Objective: The aim of present study is to evaluated CEA and CA15-3 in order to clarify at least in part their possible use as an early diagnosis tools in sera of patients with stomach, colon and rectum cancers.
Patients and methods: The study was carried out on 61 subjects comprising of 16 patients with colon cancer group (G1), 10 patients with rectum cancer group (G2), 10 patients with stomach cancer group (G3) and 25 normal healthy control The patients were selected, during the peri

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Publication Date
Thu Oct 01 2015
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Bladder exstrophy closure using anterior pelvic osteotomy of superior pubic rami

Background: Bladder exstrophy is a rare and complex urogenital malformation. The current surgical approach consists of early closure followed by other procedures later on aiming for continence. Primary closure usually requires some form of osteotomy to facilitate successful bladder and abdominal wall repair. For decades, bilateral posterior iliac osteotomy has been the most commonly used technique. A new osteotomy technique, consisting of anterior pelvic ostecotomy of the superior pubic ramus, seems to be a safe and quick alternative method to obtain tension-free approximation of the symphysis pubis
Patients and methods: A prospective study between 2006 and 2013, were 10 (9 males and 1 female) newborns underwent surgery for bladder ex

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Publication Date
Wed Jul 01 2015
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Evaluation of the proliferation marker Ki67 as a prognostic factor in patients with breast carcinoma

Background: Breast cancer is the most frequent cancer in women worldwide and in Iraq. Proliferation rates of neoplastic process can be useful in predicting prognosis, aggressiveness of cancers and to guide treatment protocols in clinical practice.
Objectives: To evaluate the role of Ki67 as a proliferative marker through analysing the associations between Ki67 with the clinic-pathological parameters, hormone receptors and Her2/neu expression.
Patients and methods: Forty paraffin blocks belonging to patient with breast carcinoma and ten blocks with benign diseases were included in this retrospective cross-sectional study and used for the immunohistochemical assessment of hormone receptors, Her2/neu and Ki67.
Results: Mean age of

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Publication Date
Fri Jul 01 2022
Journal Name
Iraqi Journal Of Hematology
Microalbuminuria among children and adolescents with sickle cell disease

BACKGROUND: Sickle cell nephropathy, a heterogeneous group of renal abnormalities resulting from complex interactions of sickle cell disease (SCD)-related factors and non-SCD phenotype characteristics, is associated with an increased risk for morbidity and mortality. AIMS: The aims of this study were to determine the frequency of microalbuminuria (MA) among pediatric patients with SCD and to determine risk factors for MA among those patients. SUBJECTS AND METHODS: A case–control study was carried out on 120 patients with SCD, 2–18 years old, registered at Basrah Center for Hereditary Blood Diseases, and 132 age-and sex-matched healthy children were included as a control group. Investigations included complete blood panel, blood urea, se

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Publication Date
Wed Nov 24 2021
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Comparison of familial and sporadic multiple sclerosis in Iraqi patients

Background: Multiple sclerosis (MS) is one of the increasing prevalent neurologic disorders. Epidemiologic and family studies implicate genetic and environmental factors in determining
susceptibility to MS. The exact effect of the former is intended for investigation in our study.
Objectives: The objective of the study is to compare the demographic features, clinical presenting features, and clinical course between familial and sporadic cases of MS.
Materials and Methods: this is a retrospective cohort study conducted in Multiple Sclerosis Center in the Medical City in Baghdad. The records of the MS center in Baghdad Teaching Hospital were surveyed, and data from 13 patients with positive family history of MS

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Publication Date
Sun Apr 01 2012
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Comparison of familial and sporadic multiple sclerosis in Iraqi patients

Background: Multiple sclerosis (MS) is one of the increasing prevalent neurologic disorders. Epidemiologic and family studies implicate genetic and environmental factors in determining
susceptibility to MS. The exact effect of the former is intended for investigation in our study.
Objectives: The objective of the study is to compare the demographic features, clinical presenting features, and clinical course between familial and sporadic cases of MS.
Materials and Methods: this is a retrospective cohort study conducted in Multiple Sclerosis Center in the Medical City in Baghdad. The records of the MS center in Baghdad Teaching Hospital were surveyed, and data from 13 patients with positive family history of MS

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Publication Date
Fri Oct 28 2022
Journal Name
Political Sciences Journal
The protest movements in Sudan and their role in the political and social transformation

This study investigates the factors that led to the outbreak of protest movements in Sudan, since Sudan’s independence in 1956 until December 2018. The study aims to identify the role of protests in bringing about political and social transformations in Sudan, besides identifying the factors that help in the success or failure of the governments that resulted from those protests, with special emphasis on the challenges of the government of the 2018 revolution. The main results of the study reveal that the December 2018 protests are the largest in term of numbers, and witness huge participation of youth and women, and the slogans raised were national and comprehensive. However, the challenges that the transitional government is going th

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Publication Date
Wed Mar 20 2024
Journal Name
Biomedical And Pharmacology Journal
Uropathogenic Infection Associated with Prostate Hypertrophy and Transurethral Resection of Prostate

Benign prostatic hyperplasia (BPH) is a prevalent condition among elderly and middle-aged men characterized by symptoms such as dysuria, urinary incontinence, and frequent micturition. The gold standard procedure for relieving BPH symptoms is transurethral resection of the prostate (TURP). However, some patients undergoing TURP are at risk of developing urinary tract infections (UTIs) due to uropathogenic bacteria. This prospective study aimed to investigate post TURP bacteruria alongside with multifactoria risk factors that implicated postoperatively compared to preoperative and intraoperative periods. Ninety patients undergoing TURP and 30 control subjects were included in the study. Urine specimen for urine analysis from pateints

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