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bsj-5822
Evaluation of some Virulence Factors and Drug Resistance of Bacteria Isolated from the Urine of Patients with TCC-Bladder Cancer

Urinary tract infections (UTIs) mean microbial pathogens in the urethra or bladder (lower urinary tract). Important risk factors for recurrent UTI include obstruction of the urinary tract, use of a bladder catheter or a suppressed immune system. This study aims to isolate and identify bacteria from patients with TCC-bladder cancer or patients with a negative cystoscope and estimate antibiotic susceptibility patterns and evaluate some of the virulence factors. From a total of 62 patients with TCC-BC or negative cystoscope, only 35 favorable bacterial growths were obtained, including Escherichia coli (UPEC), a significant bacterial isolate, and Stenotrophomonas maltophilia. The percentage of multi drug-resistance bacteria (MDR) was identified in (62.8%) while the extended drug-resistance bacteria (XDR) was (28.5%). All isolates were producer for biofilm either moderately 18/35 (49%) or strongly 18/35 (51%). Only 25/35 (71%) isolates were produced for siderophore, while 10/35 (29%) isolates were non-produced. Inducing cytochrome P450 expression protein was seen in (14/35) 40% isolates. In conclusion, patients with TCC-BC or negative cystoscope who had a urinary catheter or immune-compromised were at high risk of infecting with nosocomial or opportunistic pathogens, which could be develop antibiotic resistance, the central problem in the cohort of patients undergoing chemotherapy or immune cancer therapy

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Publication Date
Wed May 11 2011
Journal Name
Tikrit Journal Of Pure Science
Isolation and partial purification malatase isoenzymes from urine of type 2 diabetic patients

This study was performd on 50 urine specimens of patients with type 2 diabetes, in addition, 50 normal specimens were investigated as control group. The activity rate of maltase in patients (6.40±2.17) I.U/ml and activity rate of maltase in normal (0.44±0.20)I.U/ml. The results of the study reveal that maltase activity of type 2 diabetes patient's urine shows significant increase (P<0.01) compare to normal.

Publication Date
Thu Nov 23 2017
Journal Name
Brazilian Journal Of Analytical Chemistry
Microvolume-DLLME for the Spectrophotometric Determination of Clidinium Bromide in Drug, Urine, and Serum

The present study combines UV-Vis spectrophotometry and dispersive liquid-liquid microextraction (DLLME) for the preconcentration and determination of trace level clidinium bromide (Clid) in pharmaceutical preparation and real samples. The method is based on ion-pair formation between Clid and bromocresol green in aqueous solution using citrate buffer (pH = 3). The colored product was first extracted using a mixture of 800 µL acetonitrile and 300 µL chloroform solvents. Then, a spectrophotometric measurement of sediment phase was performed at λ = 420 nm. The important parameters affecting the efficiency of DLLME were optimized. Under the optimum conditions, the calibration graphs of standard -1 (Std.), drug, urine and serum were ranged

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Publication Date
Sat Dec 31 2022
Journal Name
Iraqi Journal Of Market Research And Consumer Protection
THE ROLE OF EFFLUX PUMP FOR ANTIBIOTIC RESISTANCE IN BACTERIA: THE ROLE OF EFFLUX PUMP FOR ANTIBIOTIC RESISTANCE IN BACTERIA

ABSTRACT                

The multi-drug resistant efflux pump is a glycoprotein pump whose function is to push foreign substances. The efflux pump is found in humans, animals. It also has wide-ranging properties in  bacteria and fungi. They are found in all species of bacteria, and efflux pump genes can be found in bacterial chromosomes or mobile genetic elements, such as plasmids. The most sensitive function that leads to a global problem is its resistance to antibiotics in bacterial cells, which increases the ability to bacteria from becoming strong virulence factors that most or all antibiotics cannot kill. It also has othe

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Publication Date
Mon Oct 01 2012
Journal Name
Journal Of The Faculty Of Medicine Baghdad
The value of Gray scale, color doppler and ultrasound guided- FNA in detection metastasis to the axillary lymph node in patient with primary breast cancer.

Background: Ultrasound guided fine needle aspiration (FNA) is a quick nonmorbid method of staging disease in the axilla,Color doppler ultrasound is used to differentiate benign lymph node from node that bears metastasis.
Objective: To evaluate the utility of ultrasound guided (FNA) of the axillary L.N depending on the size of the primary tumor and the appearance of the lymph node by ultrasound , and to document the difference in color Doppler flow features between benign and malignant lymph node in women with primary breast cancer.
Patients and methods: The total number of the patient in the study is (60). Data were collected about tumor size, lymph node appearance and color-power Doppler sonography compared to the result of ultras

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Publication Date
Sun Oct 27 2019
Journal Name
Iraqi Journal Of Science
Evaluation of Secondary Metabolites of Some Fungi Isolated From Beach Soils of Lagos, Nigeria Against Some Pathogens

Three beach soils in Lagos, Nigeria were screened for the presence of antibiotic producing fungi against 8 test pathogenic bacteria & fungi. The physiochemical parameters of the soils were determined following standard procedures. Soil plate dilution method was employed for isolation of marine fungi and they were identified based on cultural and microscopic characteristics. Primary screening of isolated fungi for antibiotic potential was determined by perpendicular streak method against known pathogenic test organisms (Escherichia coli, Saphylococcus aureus, Klebsiella pnuemoniae, Pseudomonas aeruginosa, Candida albicans, Saccharomyces cerevisiae,Aspergillus niger and Aspergillus fumigatus). Further sreening of the product o

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Publication Date
Wed Jan 01 2020
Journal Name
University Of Bucharest Rom Biotechnol Printed In Romania.
Publication Date
Thu Apr 27 2023
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Total L-carnitine and insulin resistance in non-obese and obese Iraqi women with polycystic ovary syndrome

Background: Polycystic ovary syndrome (PCOS) is one of the most frequent endocrine illnesses affecting reproductive - age women. L-carnitine has important roles in oxidative stress, energy production and glucose metabolism. It affects insulin resistance as decreased plasma carnitine level has been well reported in type II diabetes mellitus. Hence, it means L-carnitine may reduce insulin resistance which is found in PCO disease.

Objective: This study aims to measure the level of L-carnitine and insulin resistance in both obese and non- obese patients with PCOS.

Patients and Methods: Sixty women within the reproductive age with PCOS (30 obese and 30 non- obese) were recruited

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Publication Date
Thu Dec 13 2018
Journal Name
Iraqi National Journal Of Nursing Specialties
The Relationship between Phylogenic Typing and Antimicrobial Susceptibility Patterns forEscherichia coliIsolatedfrom UTIs atMany Hospitals in Baghdad City

Objective:The current study aime to isolate Escherichia colifrom urinary tract infections(UTIs) in many Baghdad hospitals. The study concentrate on phylogenic groups and this was done based on triplex PCRmethod by primers besieged to three genetic markers, chuA, yjaA and TspE4.C2. Evaluate the relationship of phylogenic groups of E. coli isolates with the antibiotic-non sensitive patterns. Methodology:Four hundredof E.coli bacteria isolated from urine samples from five hospitals in Baghdad city include: Ghazi AL-Hariri, Ibin- Al-Beledi , AL-Iskan , AL-Nooman and AL-Yarmoke hospitals. Phylogenetic categorizatio

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Publication Date
Fri Dec 08 2023
Journal Name
Iraqi Journal Of Science
Methicillin resistance and enterotoxigenicity of Staphylococci isolated from milk and white cheese in Iraq

Two hundred staphylococcal isolates isolated from milk and white cheese samples, which were collected from local markets in Baghdad. The predominant species was Staphylococcus aureus 97 isolates (48.5%), followed by S.chromogenes 82 (41%) and 21 (10.5%) S.epidermidis isolates. The pattern of antibiotic susceptibility of Coagulase Positive Staphylococci (COPS) and Coagulase Negative Staphylococci (CONS) isolates to 3 antibiotics (Methicillin, Tetracyclin and Vancomycin) was determined using disc diffusion method; the results revealed that 80 S. aureus isolates (82.47%) found to be methicillin resistant (MRSA) while 8 isolates (8.24%) were vancomycin resistant (VRSA) and 18 S. aureus isolates (18.5%) resist tetracycline antibiotic. Sixty f

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Publication Date
Sat Jun 30 2007
Journal Name
Al-kindy College Medical Journal
Urine Cytology in Patients with Long Standing Dialysis

Background: Dialysis is in common use to treat patients
with end stage renal failure .However longstanding dialysis
harboring some cellular changes in various body fluids.
This study was conducted in order to detect these changes
in urine.
Objective: The study was conducted to detect cellular
changes in urine of patients with longstanding dialysis.
Method: Fifty-three urine samples were examined
cytologically obtained from patients with longstanding
dialysis during 6 months period. Freshly voided midstream
urine samples were taken . Samples were centrifuged and 2
to 3 drops of sediments were smeared on 2 glass slides and
fixed in 95% ethyl alcohol then stained with Hand E stain
to be evaluated.
R

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