The current study was designed to investigate the alterations in the ultrastructure of orgenelles and cellular activity of exocrine pancreatic acini of experimentally induced-diabetic rats and to assess the usefulness of herbal combination supplementation in improving the ultrastructure and cellular activity of exocrine pancreas. The number of albino male rats used were 24 which divided into equally 4 groups; group I: control group, group II: alloxan-induced diabetes mellitus (single intraperitoneal dose of alloxan 120 mg/kg for 3 days), group III: herbal combination treatment composed from the extracts of fenugreek seeds (Trigonella foenum-graecum), black cumin (Nigella sativa) seeds, rhizomes of ginger (Zingiber officinale), leaves of olive (Olea europeae), and seeds of ash (Fraxinus ssp). Each rat given 2.5 ml (0.5 ml from each five mixed plant extracts used) for 2 months. group IV: diabetes was induced as group II and treated with herbal combination as group III for 2 months. The processing for investigating by transmission electron microscopy were carried out for all pancreata taken from all groups. There was significant reduction (p<0.05) in mean of FBG levels in diabetic rats treated with herbal combination (group IV) as compared to diabetic control and could revert FBG to normal value compared to negative control The results of examination of semithin and ultrathin sections in diabetic rats revealed many degenerative changes in the exocrine pancreatic tissue in comparison to control group. These degenerative changes can be summarized as disturbances in the arrangement of pancreatic acinar cells and decreased secretory granules in addition to the vacuolation of cytoplasm and pyknotic nuclei were observed in semithin sections. Moreover, degenerated nuclei, vacuolation of cytoplasm, fragmentation of rough endoplasm reticulum and degranulation of the most pancreatic acinar cells were noticed in ultrathin sections. In contrast, the acinar cells of group administrated with herbal combination only had normal ultrastructure, cellular activity, and nuclei, well-developed rough endoplasmic reticulum with abundant zymogen granules. Also, most of the acinar cells retrieved their normal ultrastructure and cellular activity in diabetic group treated with herbal combination and herbal combination could decrease most of the degenerative changes caused by alloxan-induced diabetes. In conclusion, the daily supplementation with this herbal combination to diabetic rat for 2 months could achieving promising effects due to its cytoprotective influence and showed the ability to decreased degenerative damage in the ultrastructure and cellular activity of exocrine pancreatic acini in type 2 diabetic rats.
The present study was conducted using 16 adult male Swiss albino mice (weighing 40-50g). They were equally divided into four experimental groups. The first group was only given tap water as control during experimentation period (40 days). Group II, group III and group IV of mice were daily given a subcutaneous injection of cobalt as cobalt chloride (CoCl2) at a dose of 20mg, 40mg, and 60mg /Kg body weight respectively. The examination of the histological sections of the second experimental group of mice (low dose group) showed no particular changes in the columnar epithelial cells of the duodenum of mice. The examination of the histological sections of the third experimental group of mice (moderate dose group) showed abundant degenerativ
... Show MoreFisetin is a plant flavonoid found in strawberries and other fruits and vegetables such as apples, persimmons, and onions. It has many pharmacological effects like anti-inflammatory, antioxidant, cardioprotective, neuroprotective, and anti-carcinogenicity which are attributed to its ability to reduce oxidative stress which considers the main reason for different disease conditions. Genotoxicity refers to the genetic material destruction within the cell which can be caused by different chemicals as well as radiation. The present study evaluates the effect of orally-administered fisetin daily for seven constitutive days on genotoxicity induced by cyclophosphamide in rats’ bone marrow and spleen cells. Results showed that fisetin exh
... Show MoreBackground: Adipose derived-mesenchymal stem cells have been used as an alternative to bone marrow cells in this study. Objective: We investigated the in vitro isolation, identification, and differentiation of stem cells into neuron cells, in order to produce neuron cells via cell culture, which would be useful in nerve injury treatment. Method: Mouse adipose mesenchymal stem cells were dissected from the abdominal subcutaneous region. Neural differentiation was induced using β-mercaptoethanol. This study included two different neural stage markers, i.e. nestin and neurofilament light-chain, to detect immature and mature neurons, respectively. Results: The immunocytochemistry results showed that the use of β-mercaptoethanol resulted in
... Show MoreThe importance of the study stems from the fact that it deals with a very important subject, namely, the pivotal role played by E-banking in achieving the competitive advantage of the banking institutions operating in Algeria. By adopting the banking institution and adopting the elements of the electronic marketing mix and developing it as required by the environment The banking system of developments will inevitably be able to achieve excellence from its competitors as each of these elements have an important role in achieving competitive advantage, we relied in this study on studies and research that directly affect the problem of the study and we have put Estep In order to activate the contribution of e-banking in achieving competitiv
... Show MoreSeventy five adult virgin female Norway rats (60 experimental and 15 controls) were used to evaluate the effect of seeds of three herbs (Fennel, Cumin and Garden cress) on their mammary glands. Experimental animals were fed with these herbs (each type of herb seeds was given to twenty experimental rats) for fourteen days. Rats were sacrificed and mammary gland sections were obtained, stained then morphometrically assessed. Serum prolactin level was performed too. Results revealed that Garden cress seeds are the strongest lactogenic agent among the three. Both Fennel and Cumin seeds were shown to be very weak galactagogues.
The present study was conducted using 15 adult male Swiss albino mice (weighing 35-45g). They were equally divided into three experimental groups. The first group was only given water as control during experimentation period (28days). Both of the second and third groups of mice were daily given a subcutaneous injection of cadmium as cadmium chloride (CdCl2) at a dose of 2mg and 4 mg Cd/Kg body weight respectively. The examination of the histological sections of the second experimental group of mice showed abundant histological changes in the hepatocytes such as increased size, presence of spaces and dense appearance of the cytoplasm, whereas these changes were more abundant in the hepatocytes of the third experimental group of mice in co
... Show MoreIntroduction: Diabetic foot infections are one of the most severe complications of diabetes. This study was aimed to determine the common bacterial isolates of diabetic foot infections and the in vitro antibiotic susceptibility then treatment.
Methods: A swab was taken from the foot ulcer, and the aerobic bacteria were isolated and identified by cultural, microscopic and biochemical test, then by api-20E system. After that their antibiotic susceptibility pattern was determined. Then local and systemic treatment was used to treat the diabetic foot patients.
Results: Bacterial isolates belonging to twelve species were obtained from diabetic foot patients. Gram (-) bacteria were the predominant pathogens in the diabetic foot infection
Background:
Wireless sensor networks (WSNs) are emerging in various application like military, area monitoring, health monitoring, industry monitoring and many more. The challenges of the successful WSN application are the energy consumption problem. since the small, portable batteries integrated into the sensor chips cannot be re-charged easily from an economical point of view. This work focusses on prolonging the network lifetime of WSNs by reducing and balancing energy consumption during routing process from hop number point of view. In this paper, performance simulation was done between two types of protocols LEACH that uses single hop path and MODLEACH that uses multi hop path by using Intel Care i3 CPU (2.13GHz) laptop with MATLAB (R2014a). Th
... Show MoreBackground: to evaluate subcutaneous pancreatic transplantation in an experimentally induced diabetes mellitus in golden hamsters.
Methods - many indexes (histological /using two stains, histochemical /using three enzymes, morphometrical and biochemical /blood glucose level) were employed in this assessment. Alloxan was used as ß-cytotoxic therapy (to induce diabetes mellitus), and cyclophosphamide was employed as immunosuppressive agent.
Results - high rate of success of transplantation (clearly evident by microscopical features of viable endocrine and exocrine tissue of transplanted pancreas and great tissue vascularization, normal enzymatic activities and significant lowering of blood glucose level