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bsj-4318
Morphological and Molecular Identification of Limnodrilus Claparede,1862 Species(clitellata: naiDIDAE) IN Tigris River, Baghdad/Iraq
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Sludge worm samples were collected from the Tigers River sediment during the period from November 2018 to June 2019 in Al Sarafiya District/ Baghdad- Iraq. Biometric morphological measurements focusing on the form of penis sheath and chaetal morphology were used for species identification, in addition to molecular analysis by amplification of conserved 18s rRNA encoding gene using ITS1 and ITS4 universal primers.According to the morphological measurement records, the results revealed the existence of Limnodrilus hoffmeisteri Claparede 1862, L. claparedeianus Ratzel, 1868 and L. cervix Brinkhurst 1963. Other two groups of specimens, with short penis sheath, were identified by molecular technology as L. hoffmeisteri complex, and L. claparedeianus.-cervix. Both species were considered as new record for Iraqi fauna, and had been registered in NCBI under the accession number LC497073 and LC497074 respectively

According to the morphological measurements records, the results revealed the existence of Limnodrilus hoffmeisteri Claparede 1862, L. claparedeianus Ratzel, 1868 and L. cervix Brinkhurst 1963, which identified by molecular analysis using 18S rRNA gene and DNA sequencing recorded  L. hoffmeisteri complex, This species is a new record in Iraq, and has been registered in NCBI under the accession number LC497073. There was also specimen identified by molecular analysis using 18S rRNA gene and DNA sequencing recorded L. Claparedeianus –cervix. Also, this species is a new record in Iraq, and has been registered in NCBI under the accession number LC497074.

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Keyword: Limnodrilus hoffmeisteri complex, Iraq, LC497073, NCBI, Limnodrilus hoffmeisteri, L. Claparedeianus, L. cervix, L. Claparedeianus –cervix, LC 497073.

*Part of M.Sc. Thesis of Research

Sludge worms samples were collected from the Tigers River sediment in Al Sarafiya district, / Baghdad- Iraq. Biometric morphological measurement focusing on the form of penis sheath and chaetal morphology, in addition to molecular analysis using 18s rRNA gene, were used for species identification.

According to the morphological measurements records, the results revealed the existence of two groups of Limnodrilus hoffmeisteri Claparède 1862 Individuals have long penis sheath with distal end with symmetrical or asymmetrical hood, while other individuals with short penis sheath with circular plate- like hood, which identified by molecular analysis as L. hoffmeisteri complex, and the isolate 18S rRNA  recorded with the accession number LC497073

Two groups of very long penis sheath identified as L. claparedeianus Ratzel, 1868, and L. cervix Brinkhurst 1963, with dorsal and ventral projections.  There are also a group of specimens with short penis sheath, which identified by molecular techniques as L. Claparedeianus –cervix 18S rRNA encoding genomic sequence was resented to the NCBI, recorded with the accession number LC497074.  Limnodrilus hoffmeisteri complex and L. claparedeianus – cervix were considered as new records for Iraq.

 

 

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Tue Oct 02 2018
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Nonlinear optical properties of polymer [PMMA] thin films doped with dye lasing compounds, Rhodamine 6G and Acriflavine in chloroform solvent by using dip coating method
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The fluorescence emission of Rhodamine 6G (R6G) and Acriflavine dyes in PMMA polymer have been studied by changing the irradiation and exposure time of laser light to know the effect of this parameter. It was found that the fluorescence intensity decreases in the polymer samples doped dyes as the exposure time increases and then reaches stabilization at long times, this behavior called photobleaching, which have been shown in liquid phase less than solid phase. Using 2nd harmonic with wavelength 530 nm laser, the photobleaching effect in the two dye-doped polymers different solvent but same was studied. It was observed that photobleaching of by different solution and by using dip spin coating the photobleaching seem in liquid phase more

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