Aeromonas salmonicida is a fish pathogen and recognized to cause a variety of diseases in humans. There are a few information about A.salmonicida in Iraq and there is no any previous molecular study on it. During the period of December 2017 to May 2018; Sixteen isolates of the A. salmonicida were isolated and identified from 300 common carp (Cyprinus carpio) fishes stomach in aquarium of Erbil city/ Iraq by using manual, automated Vitek 2 compact system, and confirmed by PCR using gene TonB-dependent siderophore (364bp). Antimicrobial susceptibility was determined by disk diffusion method and the results found that all isolates 100% susceptible to imipenem, 100% resistant to nalidixic acid and variable resistance to other studied antibiotics. The antibacterial effect of aqueous and alcohol extracts of Eminium spiculatum was studied by MIC and Molecular studies. The results found that aqueous and alcohol extracts of E. spiculatum have inhibitory effect and the MIC were 1400, 1800 µg/ml respectively. The sub MIC was used for both plant extracts, in plasmid profile the number of plasmid DNA was differ when treated with plant extracts. The inhibitory effect of the plant extracts against total proteins was studied by SDS- polyacrylamide gel electrophoresis and the results showed that there were variations in the protein bands in studied isolates and induction of new bands after treating with plant extracts. It was concluded that the leaves extracts of E.spiculatum could be used as antimicrobial for treatment of A. salmonicida infections and the results were confirmed by molecular studies.
Background: Humans skin, is the largest organ of the integumentary system, it has multiple layers of ectodermal tissue and guards the underlying muscles, bones, ligaments and internal organs. Pityriasis versicolor is the prototypical skin disease etiologically connected to Malassezia species. Malassezia furfur is the primary causative agent of pityriasis versicolor which causes either hyperpigmentation or hypopigmentation of the skin.
Objective: To identify of Malassezia furfur associated with pityriasis versicolor patients and healthy control by using molecular detection methods.
Material and Methods: Sixty patients suffering from pityriasis versicolor disease who attended Medical Imammaine Kadhmain City from beginning of 1st Dece
This study focuses on the biodegradation of oxymatrine insecticide by some soil fungi isolated from four agriculture stations. The results showed that the highest degradation rate 94.66% was recorded by Ulocladium sp. at 10 days and A. niger recorded the lowest degradation rate 45.86%, while at 20 days Ulocladium sp. also showed the highest degradation rate 94.98% and the lowest degradation rate reached to 82.49% with A.niger. The mix (Exerohilum sp.+Ulocladium sp.) recorded the highest degradation rate of oxymatrine insecticide 90.22%, 88.51%, 85.34% at 4, 8 and 12 ppm.The use of mixed isolates enhanced the biodegradation process. There is no study of oxymatrine biodegradation
... Show MoreDried imported blood worms Chironomus reparius was used to motivate the growth of young carp Cyprinus carpio L ., as fish powder was partial and total replaced by blood worms which is a component of the fodder of the common carp fish. Results have shown that blood worm partial replacement treatment surpasses the imported fish powder. Rates of growth motivation of this treatment have been higher than both the control and total replacement processes. Results have shown significant differences in the weight of the fish in the partial replacement of the fish powder by the blood worms.
Background: wound infections are associated with increased morbidity and mortality. Etiologic agents of wound infections vary with geographical location
Patients and Methods: All burn patients admitted to Specialized Burn Hospital from November 2011 to May 2012. Once fungal infection was suspected clinically, swabs were harvested for the culture of yeast. The sensitivities of the identified yeast were determined and the positive samples and cases were analyzed.Objective: This study presents Cryptococcus neoformans and their Characteristics identified from burn patients at a major Iraqi Specialized Burn Hospital.
Result: The most predominant yeast
... Show MoreMicroorganisms have an active role in biotechnology for example yeasts, especially in some genus like Saccharomyces, Pichia, and Candida. C.tropicalis one of the most important species of Candida and despite it is one of the causative agents of candidiasis but it has a major role in the production of many chemical compounds. C.tropicalis in the previous study was isolated from sheep dung and morphologically and molecularly classified the result of sequencing was elucidate 100% similarity between the studied isolate and other isolates inserted in DNA Data Bank of Japan DDBJ, physiologically this isolate tolerated 6% ethanol concentration in broth media with the ability to the pro
... Show MoreIn this study the Entrance Surface Dose (ESD) received by pediatrics patients undergoing chest, abdomen and skull X-ray examinations was estimated. The study was conducted in two hospitals in Najaf city where three radiographic systems were considered. The study participants were classified into four age groups 0-1 , 1-5 , 5-10 and 10-15 years. Calculations were performed using exposure factors, kVp, mAs and focal-skin distance, together with patient data age. The ESD was calculated for the involved patients who underwent an Antero-posterior (AP) chest, abdomen and skull X-ray examination. The resulted data were analyzed and compared with international dose references. For all studied radiographic examinations and all X-ray mac
... Show MoreThis study was carried out in the laboratories of fish and animal resource center-Agricultural Research Directorate for the period between 1/3-26/5/2015 to study the effect of total and partial substitution of white corn (WC) Sorghum bicolor germinated for different period and additive mixture of probiotic & diet enzymes of yellow corn (YC) Zea mays in common carp Cyprinus carpio L. diets. The fish fed on experimental diets contained two levels of substitution germination WC with three different periods and not germination with 0.5% mixture of probiotic+diet enzymes (50% and 100%) from YC. A 11 experimental diets were formulated, diets 1 and 2 used raw WC without germinate at two substitution levels of 50% and 100% respectively of YC
... Show MoreThe heavy metals Cd, Cu, Fe, pb, and Zn were determined in dissolved and particulate phases of the water,in addition to exchangeable and residual phases of the sediment and in the selected organs of the fish Cyprinus carpio collected from the Euphrates River near Al-Nassiriya city center south of Iraq during the summer period / 2009 .Also sediment texture and total organic carbon(TOC) were measured. Analysis emploing a flam Atomic Absorption Spectrophotometers . The mean regional concentrations of the heavy metals in dissolved (µg/l) and particulate phases (µg/gm) dry weight were Cd (0.15,16.13) ,Cu (0.59,24.48) ,Fe (726,909.4) ,Pb (0.20, 49.95) and Zn (2.5,35.62) respectively,and those for exchangeable and residual phases of the
... Show MoreThe present study aimed to estimate percentage of red and white muscles fibers in two species of bony fishes belong to two different families which represented by Tenualosa ilisha (Hamilton , 1822) that belongs to the family Clupeidae and Cyprinus carpio (Linnaeus , 1758) that belongs to the family Cyprinidae . The samples were collected from local market at holy Karbala province. The results show difference clear in mean values of muscles numbers at different studied length groups, and the percentage of red muscles fibers were less than that of white muscle fibers at different body regions (R1,R2,and R3) among species. The total average of red muscle fibers values ranged between (4.23 - 11.08 %) while the total average of wh
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