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Antimicrobial Resistance of Aeromonas salmonicida Isolated From Common carp (Cyprinus carpio) Fishes in Erbil City/ Iraq

Aeromonas salmonicida is a fish pathogen and recognized to cause a variety of diseases in humans. There are a few information about A.salmonicida in Iraq and there is no any previous molecular study on it. During the period of December 2017 to May 2018; Sixteen isolates of the A. salmonicida were isolated and identified from 300 common carp (Cyprinus carpio) fishes stomach in aquarium of Erbil city/ Iraq by using manual, automated Vitek 2 compact system, and confirmed by PCR using gene TonB-dependent siderophore (364bp). Antimicrobial susceptibility was determined by disk diffusion method and the results found that all isolates 100% susceptible to imipenem, 100% resistant to nalidixic acid and variable resistance to other studied antibiotics. The antibacterial effect of aqueous and alcohol extracts of Eminium spiculatum was studied by MIC and Molecular studies. The results found that aqueous and alcohol extracts of E. spiculatum have inhibitory effect and the MIC were 1400, 1800 µg/ml respectively. The sub MIC was used for both plant extracts, in plasmid profile the number of plasmid DNA was differ when treated with plant extracts. The inhibitory effect of the plant extracts against total proteins was studied by SDS- polyacrylamide gel electrophoresis and the results showed that there were variations in the protein bands in studied isolates and induction of new bands after treating with plant extracts. It was concluded that the leaves extracts of E.spiculatum could be used as antimicrobial for treatment of A. salmonicida infections and the results were confirmed by molecular studies.

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Publication Date
Wed Sep 01 2021
Journal Name
Baghdad Science Journal
Interleukin-1 Receptor Antagonist (IL-1RN) Gene Variable Number Tandem Repeats (VNTR) Polymorphism Association in men Infertility in Erbil City /Kurdistan Iraq

The interleukin-1 family has multifaceted roles in men٫s reproductive syste. Out  of these is interleukin-1 receptor antagonist (IL-1RN) which exists in men gonads, and in case of infection and inflammatory process, its activity is increased.  The current study aims to verify a possible linkage of Variable Number Tandem Repeat (VNTR) polymorphism of the IL-1RN gene with human men infertility. The study groups enrolled included 100 infertile men and 100 fertile and healthy men. Their seminal fluids were subjected to analysis. Also peripheral blood samples were collected for the assessment or detection of polymorphic Variable Number  Tandem Repeats (VNTR) polymorphism of interleukin-1 receptor antagonist gene (IL-1RN). Two a

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Publication Date
Mon Dec 31 2018
Journal Name
Iraqi Journal Of Market Research And Consumer Protection
STUDYING THE EFFECT OF Proteinase K AND α-Amylase ON INHIBITION OF Staphylococcus aureus AND Klebsiella pneumoniae BIOFILM ISOLATE FROM URINARY TRACT INFECTION.: STUDYING THE EFFECT OF Proteinase K AND α-Amylase ON INHIBITION OF Staphylococcus aureus AND Klebsiella pneumoniae BIOFILM ISOLATE FROM URINARY TRACT INFECTION.

The current study aimed to investigate the viability of biofilm formation klebsilla pneumoniae and Staphylococcus aureus. 440 urine samples were collected from patients suffering from urinary tract infection (UTI) from those who were admitted and visitors to Al-Ramadi Teaching Hospital, Al-Yarmouk Teaching Hospital, Al-Ramadi Teaching Hospital for women and children and , Teaching Laboratories in the Medical City for both genders for a period extended from 5 July, 2017 to 10 October, 2017. Samples were diagnosed by culturing them on a selective media and by biochemical testes , also, diagnosis was ensured by using VITEK-2 compact system. Results showed that K.pneumoniae isolation ratio was 17.1%(68) and S.aureus ratio was 13.1%(52). Thei

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Publication Date
Mon May 31 2021
Journal Name
Indian Journal Of Ecology
Identification and Antimicrobial Susceptibility Profiles of Salmonella spp. Isolated from Chicken Flocks and their Feed and Water in Karbala, Iraq

The objective of study was determining the most prevalent Salmonella spp. and their antimicrobial susceptibility in broilers and laying chickens and their feed and drinking water in five chicken farms in Karbala, Iraq over the period from August to October 2020. A total of 289 samples, including 217 cloaca swabs, 46 water and 26 feed samples were collected. Salmonella spp. was identified firstly by routine diagnostic methods, followed by applying the API 20E kit, the Vitek2 system, and serology. There was significant differences in Salmonella prevalence among different types of samples, mainly cloaca swabs reported a high isolation rate (21.7%). In contrast, feed samples were completely free of contamination. The highest rate of isolation w

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Publication Date
Sun Aug 13 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Parasites of Fishes Collected from Tigris River, Salah AlDeen Province, Iraq

A total of 109 fish specimens belonging to six species (Barbus grypus, B. luteus, B. xanthopterus, Cyprinion macrostomum, Cyprinus carpio and Liza abu) were collected from Tigris river passing through Salah Al-Deen province during the period from September 2008 till January 2009. These fishes were infected with nine parasite species which included two sporozoans (Myxobolus mülleri and Dermocystidium percae), three ciliates (Trichodina cottidarum, T. elegini and T. murmanica) and four monogenetic trematodes (Dactylogyrus rhodeianus, D. vastator, Paradiplozoon cyprini and Microcotyle donavini). Among these parasites, two trichodinids (T. elegini and T. murmanica) were reported for the first time from fishes of Iraq. Also, B. grypus is c

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Publication Date
Sat Nov 07 2020
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Determination of Factors Associated with Low Birth Weight among Babies Born in Sulaimania City, Kurdistan-Iraq

Background: Low birth weight (LBW) is the main leading cause of infant death. It is contributing to a variety of short and long term poor health outcomes. Determination of risk factors associated with LBW is important to select a suitable action to prevent or reduce this outcome. Studies on LBW and maternal risk factors in the Kurdistan region of Iraq are scarce.
Objectives: This study aimed to determine risk factors associated with Low birth weight in Sulaimania city, Kurdistan region of Iraq.
Cases and Methods: This study was carried out in the Maternity Hospital in Sulaimania from first of July, 2019 to first of February, 2020. Participants were 300 randomly selected mothers who gave a live birth. The questionnaire form, which c

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Publication Date
Sun Feb 05 2017
Journal Name
Iraqi Journal Of Market Research And Consumer Protection
STUDY THE OPTIMUM CONDITIONS FOR THE REMOVAL OF HEAVY METAL ELEMENTS FROM AQUEOUS SOLUTIONS USING CONTAMINATED BACTERIA Bacillus subtilis LOCALLY ISOLATED: STUDY THE OPTIMUM CONDITIONS FOR THE REMOVAL OF HEAVY METAL ELEMENTS FROM AQUEOUS SOLUTIONS USING CONTAMINATED BACTERIA Bacillus subtilis LOCALLY ISOLATED

We studied the effect of certain environmental conditions for removing heavy metal elements from contaminated aqueous solutions (Cd, Cu, Pb, Fe, Zn, Ni, Cr) using the bacterium Bacillus subtilis to appoint the optimal conditions for removal ,The best optimum temperature range for two isolate was 30-35○C while the hydrogen number for the maximum mineral removal range was 6-7. The best primary mineral removal was 100 mg/L, while the maximum removal for all minerals was obtained after 6 hrs of Cu element time and the maximum removal efficiency was obtained after 24 hrs of Cu element. The results have proved that the best aeration for maximum removal was obtained at rotation speed of 150 rpm/minute. Inoculums of 5ml/100ml which contained 1

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Publication Date
Fri Feb 01 2019
Journal Name
Journal Of Economics And Administrative Sciences
This study sought to determine the impact of spreading the culture of quality in improving the quality of tourism service in some hotels in Erbil governorate

This study sought to determine the impact of spreading the culture of quality in improving the quality of tourism service in some hotels in Erbil governorate. The theme of excellence in providing service and attention to it has become the concern of most organizations in general and tourism service organizations in particular. Tourists try to establish the mental status of customers about their tourism organizations had to pay attention to the quality of service provided to the customer and work to develop them to the advanced stages of satisfaction and happiness of customers and this will certainly positively affect the improvement of service in the tourism sector, Of the research variables were examined statistically by distrib

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Publication Date
Mon Apr 30 2012
Journal Name
Journal Of Planner And Development
RAPID LAND TENURE ASSESSMENT OF BAGHDAD CITY IN IRAQ 2010

The purpose of this study is to describe the extent and nature of informal tenure practices in urban areas in Iraq, through undertaking a rapid assessment in Baghdad city. The UN-HABITAT 2008 publication Secure Land Rights for All discusses the importance of access and rights to land throughout the developing world. Secure land rights are critical to development and poverty reduction, and the greatest challenge in providing secure land rights are in urban areas, where overcrowding can lead to a number of informal tenure practices ranging from individually unregistered or unauthorised housing, to large informal settlements. Access to land is a fundamental basis for human shelter, food production, and other economic activity. Secur

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Publication Date
Mon Apr 23 2018
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Antimicrobial activity of some plants extracts on bacteria isolated from acne vulgaris patients

Background: Acne is a cutaneous pleomorphic disorder skin disease most frequently occurring during the adolescent in ages of 12-24, with estimated  percentage ( 85%) . There are different ways to treat acne such as  using of antibiotics  , herpes , and mixing treatments .

Methods : Antibacterial activity  of  four concentrations (100,50,25,12.5)mg /ml of  alcoholic  and cold  aqueous  crude extracts of Cinnamon(Cinnamomum verum ), Henna (Lawsonia inermis ) , Lupine (Lupinus luteus) were studied against aerobic and&nbs

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Publication Date
Wed Jun 01 2022
Journal Name
Baghdad Science Journal
Investigating the Effect of Drugs of Chloramphenicol and Gentamicin on Acanthamoeba genotype T3 Causing keratitis Isolated from Environmental samples in vitro

This study is an investigation of the drugs effect on some pathogenic Acanthamoeba isolated from Iraqi waters, where the problem of environmental adaptation that characterizes this organism in addition to being a reservoir for many pathogenic microorganisms that take shelter in it to escape disinfectants and medicines is sometimes difficult to treat it with traditional treatments.  Twenty water samples were collected from different water regions in Iraq, namely the Dokan Lake, Tigris River, Euphrates River and Najaf Sea, 5 samples from each source.  Acanthamoeba was isolated from water samples on NNA and PYG media, using an inverted microscope with an electron microscope to determine their phenotypic features. PCR and

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