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Application of Randomly Amplified Polymorphic DNA (RAPD) Technique to Estimate Genetic Distance among Some Methicillin Resistant Staphylococcus aureus Isolated from Different Iraqi Hospitals
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Methicillin resistant Staphylococcus aureus (MRSA) is one of the principal nosocomial causative agents. This bacterium has the capability to resist wide range of antibiotics and it is responsible for many diseases like skin, nose and wounds infection. In this study, randomly amplified polymorphic DNA (RAPD)-PCR was applied with ten random primers to examine the molecular diversity among methicillin resistant Staphylococcus aureus (MRSA) isolates in the hospitals and to investigate the genetic distance between them. 90 Isolates were collected from clinical specimens from Iraqi hospitals for a total of 90 isolates. Only 10 strains (11.11%) were found to be MRSA. From these 10 primers, only 9 gave clear amplification products. 91 fragment lines were generated from these primers across all isolates with an average of 10 fragment lines per primer. Of these, 90 (99%) were polymorphic. The size of the amplified bands ranged between 145-2109 bp. The polymorphism percentage for all primers was 100% except OP-X17 primer which gave 86% polymorphism. The genetic distances revealed from Jaccard similarity index was calculated for the 90 RAPD polymorphic fragment lines. The highest genetic distance value 0.959 was between isolate number (1) and (5) and between isolate number (3) and (10), while the lowest genetic distance value 0.218 was between isolate number (6) and (7). This study shows that RAPD-PCR technique assayed with nine primers can be successfully applied to reveal the genetic distances among methicillin resistant Staphylococcus aureus (MRSA) isolates from different hospitals.

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Publication Date
Wed Aug 30 2023
Journal Name
Iraqi Journal Of Science
The Potential Efficiency of Bacillus subtilis AIK to Remove Nickel from Aqueous Solutions
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In this study a new strain of mesophilic Bacillus subtilis AIK, recorded for the first time in Iraq, was used to remove nickel (Ni) from aqueous solutions. The factors that affect bioremediation include temperature, pH value and metal concentrations. The results showed that the highest removal efficiency (R%) was 54, 52 and 48% at 25⁰C and pH of 5, 7 and 9, and with 10 ppm Ni concentration respectively. Whereas the highest R% recorded was 47, 45 and 52% at 30⁰C and of pH 5, 7, and 9 with 1 ppm Ni concentration respectively. On the other hand, the highest R% at 40⁰C was 49, 46, 42 % at pH 5, 7 and 9, with 5, 10 and 10 ppm Ni concentrations respectively. The results also showed that the optimum pH value for Ni removal at bot

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Publication Date
Tue Jan 11 2022
Journal Name
Iraqi Journal Of Science
Methods and Simulations used to Detect Photons from Exoplanets of a Parent Star
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The extrasolar planets in the vicinity of stars are expected to be bright enough
and are very difficult to be observed by direct detection. The problem is attributed to
the side loops of the star that created due to the telescope diffraction processing.
Several methods have been suggested in the literatures are being capable to detect
exoplanet at a separation angle of 4λ/D and at a contrast ratio of 10-10. These
methods are more than one parameter function and imposing limitations on the inner
working distance. New simple method based on a circular aperture combined with a
third power Gaussian function is suggested. The parameters of this function are then
optimized based on obtaining a minimum inner working dis

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Publication Date
Sat Jul 31 2021
Journal Name
Iraqi Journal Of Science
Manufacture of Spongy Gravel from Obsidian to Produce Lightweight and Thermal-Insulating Concrete
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    The present study aims to convert obsidian rocks into spongy gravel for the use in the production of lightweight and heat insulating concrete. The rocks were burned at 960°C to achieve maximum swelling of the samples, then broken into gravel and sand sizes. For comparison purposes, two other types of aggregates were used, namely pumice and basalt. The main physical tests, such as specific gravity, bulk density, porosity, and water absorption were performed. For testing the resistance of samples to alkalinity, KOH and Na OH solutions were used. The results showed that the obsidian sample gave the best specifications, where its specific gravity was 0.33, while the values were 1.1 for pumice and 2.7 for basalt, with the

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Publication Date
Sat Sep 30 2023
Journal Name
Journal Of Accounting And Financial Studies ( Jafs )
The effect of operational efficiency on some financial indicators according to the CAMEL model of banking financial stability: An applied research on a sample of Iraqi private banks for the period 2010-2020
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Abstract

          The research aims to determine the nature of the Iraqi market in terms of banking financial stability and the extent impact of the operational efficiency on it, Accordingly, chosen 15 relational banks were chosen as an intentional sample that could represent the Iraqi banking system for the period 2010-2020. The operational efficiency variable was measured according to the data envelope model, and banking financial stability used  CAMELS model which includes five indicators (capital adequacy, asset quality, management quality, profitability, and liquidity), so for testing the research hypotheses used the random regression model by adopting the S

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Publication Date
Wed Jun 29 2022
Journal Name
Journal Of Al-rafidain University College For Sciences ( Print Issn: 1681-6870 ,online Issn: 2790-2293 )
The Use Of Genetic Algorithm In Estimating The Parameter Of Finite Mixture Of Linear Regression
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The estimation of the parameters of linear regression is based on the usual Least Square method, as this method is based on the estimation of several basic assumptions. Therefore, the accuracy of estimating the parameters of the model depends on the validity of these hypotheses. The most successful technique was the robust estimation method which is minimizing maximum likelihood estimator (MM-estimator) that proved its efficiency in this purpose. However, the use of the model becomes unrealistic and one of these assumptions is the uniformity of the variance and the normal distribution of the error. These assumptions are not achievable in the case of studying a specific problem that may include complex data of more than one model. To

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Publication Date
Thu Jun 02 2011
Journal Name
Ibn Al-haithem Journal For Pure And Applied Sciences
On modified pr-test double stage shrinkage estimators for estimate the parameters of simple linear regression model
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Publication Date
Fri Dec 01 2017
Journal Name
Journal Of Economics And Administrative Sciences
A Comparison between Methods of Laplace Estimators and the Robust Huber for Estimate parameters logistic regression model
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The logistic regression model regarded as the important regression Models ,where of the most interesting subjects in recent studies due to taking character more advanced in the process of statistical analysis .                                                

The ordinary estimating methods is failed in dealing with data that consist of the presence of outlier values and hence on the absence of such that have undesirable effect on the result.    &nbs

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Publication Date
Thu Aug 17 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Application of Kass' Snake in Medical Images Segmentation
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A   snake   is   an   energy-minimizing   spline   guided   by   external

constraint forces and influenced by image forces that pull it toward features such as lines and edges. Snakes are active contour models: they lock onto nearby edges, localizing them accurately. Snakes provide a unified account of a number of visual problems, including detection of edges, lines, and motion tracking. We have used snakes successfully for segmentation, in  which  user-imposed  constraint forces guide the snake near features of interest (anatomical structures). Magnetic Resonance Image (MRI) data set and Ultrasound images are used for our experiments.

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Publication Date
Sun Jun 01 2014
Journal Name
Baghdad Science Journal
Synthesis and Environmental Application of BiOI/BiOCl Composites
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This work illustrates an enhanced visible light photocatalytic degradation of methyl orange dye (M.O.) by employing BiOI / BiOCl composites prepared under room temperature and without any organic precursors. Various experimental parameters have been studied, namely; composition of the composite, irradiation time and cell material. Composition D which implied 75% BiOI and 25% BiOCl has shown the highest bleaching of M.O. dye. This confirms the optimum photo-sensitization phenomenon for this composition in comparison to others. In the optimum photo-sensitized composite the electron of the conduction band reveals better reducing power and the hole of the valence band exhibits more oxidative power than those of pure BiOI electron and hole. Acco

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Publication Date
Sun Jun 07 2009
Journal Name
Baghdad Science Journal
Application of delay integral equations in population growth
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In this paper, the delay integral equations in population growth will be described,discussed , studied and transfered this model to integro-differential equation. At last,we will solve this problem by using variational approach.

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