Genotypic Study of Two Virulence Factors fimH and kpsMTII in Uropathogenic Escherichia coli Isolates from Children Patients with Urinary Tract Infections

Zahraa A. Abed; Khalid A. Habib; Zaid N. Abass

doi:10.21123/bsj.2014.11.4.1475-1480

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Publication Date

Sun Dec 07 2014

Journal Name

Baghdad Science Journal

Volume

11

Issue Number

4

DOI

10.21123/bsj.2014.11.4.1475-1480

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Genotypic Study of Two Virulence Factors fimH and kpsMTII in Uropathogenic Escherichia coli Isolates from Children Patients with Urinary Tract Infections

Uropathogenic Escherichia coli (UPEC)

Polymerase chain reaction (PCR)

Virulence factor (VF).

Zahraa A. Abed

Khalid A. Habib

Zaid N. Abass

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Adhesion (type 1 fimbriae) and host defense avoidance mechanisms (capsule or lipopolysaccharide) have been shown to be prevalent in Escherichia coli isolates associated with urinary tract infections. In this work, 50 uropathogenic Escherichia coli (UPEC) isolated from children with urinary tract infections were genotypically characterized by polymerase chain reaction (PCR) assay. We used two genes; fimH and kpsMTII, both of them previously identified in uropathogenic E.coli (UPEC) isolates. The PCR assay results identified fimH (90.0)% and kpsMTII (72.0)% isolates. In the present study, was also demonstrated that these genes may be included in both or one of them within a single isolate.

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Publication Date

Thu Dec 01 2022

Journal Name

Baghdad Science Journal

Investigation of the association of AGTR1 A1166C rs5186 and FTO rs9939609 polymorphisms with the obesity in children and adolescents

AGTR1 A1166C (rs5186)

Children and adolescents

FTO rs9939609

Obesity

Polymorphism

Karantysh G.

Teplyakova E.

Bocharova O.

Karantysh G.

Shkurat T

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Obesity is a risk factor for a number of chronic conditions. Obesity is clinically defined using the body mass index (BMI) as weight in kg divided by (height)² in m² correlated with obesity. Currently, genetic markers of obesity are being studied. This study focused on the association between the angiotensin II receptor AGTR1 gene (A1166C) and fat mass and obesity-associated protein also known as alpha-ketoglutarate-dependent dioxygenase (FTO) (rs9939609) in obese children and adolescents patients in Rostov region, Russia. Five-hundreds of Russian nationality child and adolescent were recruited for the obesity-control studies. The relationship between the A1166C polymorphism of the AGTR1 gene in

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(2)

Publication Date

Mon Jan 01 2024

Journal Name

Anais Da Academia Brasileira De Ciências

Morphological study for Accipitrid birds (Accipitridforms, Accipitridae) in Iraq; part two

A part two of Accipitridae Family members that belong to three genera: Circus

Gyps

and Neophron were documented as morphometrics

distribution ranges

and conservation status were reviewed and comprehensively discussed. A total of 29 voucher specimens belong to six species which are conserved in the “Iraq Natural History Research Center and Museum INHM” as follow: Marsh Harrier Circus aeruginosus (Linnaeus

1758)

Hen Harrier Circus cyaneus (Linnaeus

1766)

Montagus Harrier Circus pygargus (Linnaeus

1758)

Pallid Harrier Circus macrourus (Gmelin

1770)

Egyptian vulture Neophron percnopterus (Linnaeus

1758)

and Griffon vulture Gyps fulfus (Hablitz

1783). The results revealed significant criteria about near threatened NT species IUCN and Birdlife International listed as Pallid Harrier Circus macrourus

and Endangered (EN) species as Egyptian vulture Neophron percnopterus globally decreasing population trend.

HIND D.

KHALIDA I.

AFKAR M.

SUHAD Y.

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Publication Date

Thu Apr 01 2021

Journal Name

Biochemical & Cellular Archives

Impacts of Starvation Stress on Biofilm Formation and expression of Virulence Genes in Mono-and Mixed-species cultures of Pseudomonas Aeruginosa and Staphylococcus aureus

W.A.

Ahmed Saad

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Staphylococcus aureus and Pseudomonas aeruginosa are the major globally distributed pathogens, which causes chronic and recalcitrant infections due to their capacity to produce biofilms in large part. Biofilm production represents a survival strategy in these species, allowing them to endure environmental stress by altering their gene expression to match their own survival needs. In this study, we co-cultured different clinical isolates of S. aureus and P. aeruginosa as mono- and mixed-species biofilms in a full-strength Brain Heart Infusion Broth (BHI) and in a 1000-fold diluted Brain Heart Infusion Broth (BHI/1000) using Microtiter plate assay and determination of colony-forming units. Furthermore, the effect of starvation stress on the e

(3)

Publication Date

Sun Apr 30 2023

Journal Name

Al-kindy College Medical Journal

Comparison between Reference Infliximab (Remicade) and its Biosimilar (Remsima) in Patients with Ankylosing Spondylitis: A Field-based Pharmacoeconomic Study

Cost-effectiveness

Ankylosing spondylitis

Infliximab

Biosimilar

Quality of life

Hasan Raid

Ali Azeez

Nizar

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Background: Ankylosing spondylitis is a chronic inflammatory disease that mostly involves the spine and sacroiliac joints. It is associated with a decreased quality of life. Biological medicines such as infliximab and its biosimilar are the mainstay treatments for active ankylosing spondylitis.

Objective: The study objective was to conduct a pharmacoeconomic study comparing the cost-effectiveness of the reference infliximab with its biosimilar in ankylosing spondylitis patients visiting public hospitals.

Subjects and Method: This is a two-center pharmacoeconomic study performed at two large teaching governmental hospitals in Baghdad, Iraq, which s

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(4)

(5)

Publication Date

Sun Dec 01 2019

Journal Name

Zeitschrift Für Assyriologie Und Vorderasiatische Archäologie

Literary Texts from the Sippar Library I: Two Babylonian Classics

Anmar Abdulillah

Enrique

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Abstract<p>Publication and edition of two tablets from the library in the Ebabbar Temple of Sippar, a manuscript of the ‘Babylonian Poem of the Righteous Sufferer’ (<italic>Ludlul bēl nēmeqi</italic>) Tablet III, and a manuscript of the long prayer to Marduk ‘Furious Lord’ (also known as ‘Marduk 1’).</p>

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Publication Date

Mon Mar 13 2017

Journal Name

International Journal Of Healthcare Management

Theories and factors applied in investigating the user acceptance towards personal health records: Review study

Ali

Abdul Samad

Gede Pramudya

Malik Bader

Yasir Hamad

Mohamed

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(26)

Publication Date

Wed Mar 29 2017

Journal Name

Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)

In vitro MIC of Itraconazole Against Different Isolates of Candida albicans

Inssaf

...Show More Authors

In vitro antifungal susceptibility test of itraconazole was carried out against 38 isolates from nails, skin, oral cavity, vagina and wounds, This study was done in Ramadi Teaching Hospital in period from January to August 2010. According to the National Committee for Clinical Laboratory Standard (NCCLS ) M 27- A by using the broth dilution method. Inoculum size was 1-5X10³ CFU/ ml, while final concentrations of itraconazole ranged from 0.025 â€“ 6.4 Î¼g / ml by using RPMI â€“ 1640 broth media and the fungus was incubated at 35 ^oC. No resistant stain was recorded. MIC ranged from 0.05 â€“ 6.4 Î¼g / ml and the Mean Â± SEM was 0.89 Â± 0.28. MIC for nail isolates was 0.05 â€“

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Publication Date

Thu Jun 01 2023

Journal Name

Online Journal Of Veterinary Research

Article review: Infections by gram + bacteria in laboratory mice

gram positive

bacteria

laboratory

mice

Mohammed A.K.

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Publication Date

Tue Mar 30 2021

Journal Name

Baghdad Science Journal

Hypothyroidism and AMH in Iraqi Patients with Chronic Kidney Disease

CKD

Hypothyroidism

TSH

T3

AMH.

Noori Mohammed

Athraa K.

Ali J.

...Show More Authors

This study was conducted to investigate thyroid function and Anti-Müllerian hormone (AMH) in (Chronic kidney disease) CKD patients by evaluating their levels in CKD patients, 50 patients were diagnosed to have CKD stage-5, their ages ranged between 20-50 years (25 males and 25 females) who attended the Nephrology and Transplant Center in Medical City of Baghdad- Iraq, they were recruited from April 2018 to July 2018 and were enrolled into the study. The control group consisted of 20 healthy individuals, their ages ranged between 20-48 years (10 males and 10 females). The study showed non-significant (p>0.05) increase in AMH level in CKD patients compared to the control group. On the other hand, TSH was recorded a highly significant (

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(2)

Publication Date

Sun Jun 30 2013

Journal Name

Al-kindy College Medical Journal

HLA Typing in Iraqi Patients with Obesity and Primary Osteoarthritis.

HLA-DRB Osteoarthritis Obesity

Sadiq Abbas

Faris

Haider Hashim

Batool Mutar

Hyam

Laheeb Ali

Zena

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Background: Osteoarthritis (OA) is a degenerative joint disease. It is one of the major causes of disability in developed and developing countries. Human leukocyte antigen (HLA) as part of immune system has a role in the disease process.Objectives: To investigate whether there is an association between HLA class II-DRB and OA.Methods: A case control study with 26 patients with osteoarthritis and 22 apparently healthy obese control persons matching in ethnicity were enrolled in this study during the period between October 2012 till March 2013. Direct interview was done with each patient and HLA typing was done by molecular method using Sequence Specific Primer (PCR-SSP) method using One Lambda Kit-USA. Results: The results showed that fem

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