1-Objective:- Polyphenols are biochemical compounds with antioxidant activity against differences diseases related to Lipid peroxidation such as diabetes mellitus. Polyphenols distributed widely in medical plants, the aim of the study is to extract and analyze some polyphenolic compounds from grape seeds and examine their effects on (STZ) induced diabetic mice. 2-Methods:- In the present study , a group of polyphenols has been extracted from Iraqi grape seeds by ethanol and the extract has been analyzed by using High Performance Liquid Chromatography (HPLC) coupled to ultra violet (UV) detection. Five fractions were eluted from the column : procyanidin B1, gallic acid , quercetin , catechin and epicatechin. The detection was recorded at (280 nm). The reactive action of the above polyphenols on glucose, and malondialdehyde (MDA) levels and glutathione – S – transferase (GST) activity was tested in (30) streptozotocin (STZ) induced diabetic mice which treated with extracted grape seeds polyphenols to examine the antioxidant effect of these compounds . Grape seeds polyphenols action on the above parameters was determined before treatment and after (1 week), ( 2 weeks) and (3 weeks) of treatment with this reactive extract. 3-Results:- The data have shown that glucose levels were increased in (30) diabetic mice (after injection with streptozotocin) compared with control group (p<0.001) but after treatment with grape seeds polyphenols , glucose levels were decreased relatively (p<0.001) after (1 week), (p<0.001) after (2 weeks) and (p<0.01) after (3 weeks) of treatment with these polyphenolic compounds. MDA levels were increased for diabetic mice (after injection with streptozotocin) compared with control group (p<0.001) but after treatment with grape seeds polyphenols , MDA levels were decreased relatively (p<0.01) after (1 week) ,(p<0.01) after (2 weeks) and (p<0.05) after (3 weeks) of treatment. GST activity was increased in diabetic mice (after injection with streptozotocin ) compared with control group (p<0.001) but after treatment with grape seeds polyphenols , GST activity was decreased relatively (p<0.01) after (1 week), (p<0.01) after (2 weeks) an (p<0.05) after (3 weeks)of treatment with these antioxidant compounds. 4-Conclusion:- Our data indicated that glucose , MDA levels and GST activity correlated positively with oxidative stress related to diabetes mellitus.
Background: Cisplatin is a potent anti-cancer agent used successfully in treatment of cancers of solid organs, but it has a high rate of nephrotoxicity.
Objective: The present study was designed to study Cisplatin-induced nephrotoxicity and the nephroprotective property of pomegranate juice.
Materials and Methods: The experiment was performed on 36 Iraqi white male domestic rabbits. Rabbits were divided into three groups; control group (received neither pomegranate juice, no Cisplatin), Cisplatin group (received Cisplatin only), and pomegranate group (received pomegranate juice and Cisplatin).
Results: Cisplatin group showed marked reduction of renal function manifested by high levels of blood urea, serum creatinine, and low lev
In this work, seven soil samples were brought brought to study and analyses the element concentrations from different southern regions of Iraq using laser-induced breakdown spectroscopy (LIBS) technique. It has been documented as an atomic emission spectroscopy (AES) technique. Laser-induced plasma utilized to analyze elements in materials (gases, liquids, and solids). In order to analyze elements in materials (gases, liquids, and solid). The Nd: YAG laser excitation source at 1064 nm with pulse width 9 ns is used to generate power density of 5.5 x 1012 MW/mm2, with optical spectrum in the range 320-740 nm. From this investigation, the soil sample analysis of the southern cities of Iraqi, it is concluded that the rich soil element of P, Si,
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spectra and J>hysical methods,selected metals,which were Cu11
Zn 11
Mn11,Co11,Fe" and Hg11 were reacted with ligand to &
: Partial purification of phosphoenolpyruvate carboxykinase (PEPCK) from type 2 diabetic patients sera take place using some purification steps such as participation with ammonium sulphate (55-80%) and filtered through dialysis, then ion exchange chromatography by DEAE sepharose anion column, gel filtration chromatography by sephadex G-100 column. In ion exchange step, there are four peak are obtained, the highest enzyme activity obtained by (0.4 M Nacl) with purification fold (2.18), yield (44.3) of enzyme and specific activity (13.5) mg/ng, which obtained a single peak by gel filtration chromatography, the degree of purification (5.34) fold, yield of enzyme (20%) with specific activity (33.109mg/ng). The purified enzyme had an optimum tem
... Show MoreFrom a group of 60 patients with dentoalveolar infections among which 10 were diabetic and 10 non-diabetic were elected as test group as well as 10 normal subjects as control group. Six Staphylococcus aureus and Streptococcus anginousus were diagnosed in the first and second group of the patients the immune status of the patients and control subject were tested by pathogen specific antibody titre, neotrophil NBT reduction phagocytosis and leukocyte inhibition LIF. Diabetic patients with dentoalveolar infection shows decreased specific antibody titers, subnormal neutrophil NBT phagocytic % as well as non significant LIF % in comparison non diabetic reveal high specific antibody titers against , high neutrophil NBT% and significant LIF% re
... Show MoreBackground: This study aimed to use the combined mesio-distal crowns widths of maxillary incisors and first molars as predictors to the combined mesio-distal crowns widths of maxillary and mandibular canines and premolars. Materials and methods: The sample included 110 Iraqi Arab subjects with an age ranged between 17-25 years and class I skeletal and dental relations. The crown widths of maxillary teeth and mandibular canines and premolars were measured at the largest mesio-distal dimension on the study casts using digital electronic caliper with 0.01 mm sensitivity. Pearson’s correlation coefficient was used to determine the relation between the combined mesio-distal crowns widths of maxillary incisors and first molars and the combined
... Show MoreBackground: Despite the fact that asthma is a long-term disease that may be treated, many people are unable to control their symptoms due to a lack of knowledge about their condition. The study's purpose was to find out if a pharmacist intervention improved asthma management because of this.
Objective: this study designed to assess the effect of pharmaceutical care on pulmonary functions test.
Method: The study was completed in three months. The patients who were enrolled were divided into two groups: Group 1 consists of 23 asthma patients who were randomly assigned to receive conventional therapy for chronic bronchial asthma based on disease stage and se
... Show MoreKlebsiella pneumoniae are Gram-negative which cause many diseases such as urinary tract infections, respiratory tract infections and septicemia. Inulinase is an enzyme used in food manufacture and pharmaceuticals. Inulinase is used in decreasing lipid ratio and, cholesterol in blood and considered as a prebiotic factor inside intestine. Many microorganisms can produce inulinase, such as yeast, fungi and bacteria; among such bacteria: Bacillus spp., Arthrobacter spp., and Pseudomonas spp. but there are no studies about inulinase production by K. pneumoniae have been reported. So the current study aims at investing the ability of producing and purification inulinase by K. pneumoniae. Method: K. pneumoniae were isolated from many hospitals and
... Show MoreObjectives: Dickkopf-1 (DKK-1) is WNT/b-catenin pathway antagonist which plays a detrimental role in the development of diabetic retinopathy (DR). This research aimed to assess serum DKK-1 levels in diabetic patients who have and have not developed DR and, compare them with the control subjects finding out whether we can use it as an indicator for DR early diagnosis and to find out which one of the widely used two groups of antidiabetic treatments had the greater effect on this biomarker and hence on the progression of DR. Methods: The study participants were divided into two subgroups: First, 70 patients (36 male, 34 female) with type 2 diabetes mellitus, among them 35 patients diagnosed with DR and 35 with no evidence of DR, and s
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