A total of 551 water samples (drinking and raw water) were collected In this study, Aeromonas.hydrophila, were detected by biochemical tests and PCR (16s rRNA gene). The results of identification showed that A.hydrophila had recovery rate 63 isolates (49.21%). The results revealed that all A.hydrophila isolates were PCR positive or the 16S rRNA gene and the results of sequencing showed that two isolates of A.hydrophila(local isolates) had percentage similarities 100% with A. hydrophila ATCC 7966 in GenBank database .All strains had a minimal Inhibitory Concentration(MIC) distribution pattern for lead cetate rranged (900-1200 μg/ml), and mercury chloride ranged (40-80 μg /ml).

Failure in asphalt mixture and distress in pavement are major issues to roads infrastructure. Selecting an appropriate chemical composition of asphalt cement is a key component in avoiding these issues. This work aimed to investigate the effect of the chemical composition of different polar fractions on the rheological and physical properties of asphalt cement. Four types of asphalt cement with penetration grades of 20/30, 40/50, 60/70 and 85/100 were divided into four fractions. Complex shear modules, rutting resistance and rotational viscosity of the asphalt cement were determined by using a Dynamic Shear Rheometer and a Rotation Viscometer, respectively. The results show that an increase in the asphaltene content and Gastel index resulte

The aim of this study was to increasing natural carotenoides production by a locally isolate Rodotorula mucilagenosa M. by determination of the optimal conditions for growth and production of this agents, for encouragest to use it in food application permute artificial pigments which harmfull for consumer health and envieronmental. The optimal condition of carotenoides production from Rhodotorula mucilaginosa M were studied. The results shows the best carbon and nitrogen source were glucose and yeast extract. The carotenoids a mount production was 47430 microgram ̸ litter and 47460 microgram ̸ litter, respectively, and the optimum temperature was 30°C, PH 6, that the carotenoides a mount was 47470 microgram ̸ litter and 47670 microgr

  The present study aimed to try to find natural substances stimulate the production of bacteriocin, as well as "for detection of bacteriocin producing isolates. Two hundred and eighty ( 280)  bacterial isolates, gram negative only, were collected from 760 different pathogenic samples, consist: (Urinary tract infection, septicemia, Vaginal inflammation and diarrhea).  The isolated bacteria are: Escherichia coli, Klebsiella  pneumonia Pseudomonas  aeruginosa,, Salmonella typhi, Enterobacter cloacae, Acinetobacter baumannii, Serratia  liquefaciens, Citrobacter  freundii,  Proteus  mirabilis and Serrattia  odorifera.  Cup assay method was  used to detect bacteriocin production. Loc

     Aeromonas hydrophila is widely distributed throughout the world and causes diseases to animals and human exposed to contaminated environments such as water and soil. This study aimed to compare between isolates of A. hydrophila collected from clinical and environmental samples, through investigating the phenotype of some virulence factors in vitro, including hemolysin, protease, lipase, nuclease and biofilm formation ability. Also, the antimicrobial susceptibility for different antibiotics was determined using disc diffusion method. For genotypic identification of isolates and phylogenetic tree construction, 16S rDNA target gene was amplified and sequenced. The phenoty

The ability of A.hydrophila isolates (63 isolates) to form biofilm, was studied and the results showed that fifty six isolates (88.8%) gave positive results in Congo red agar, while 51 isolates ( 80.7%) gave positive results in Christensen method, sixty isolates( 95.2%) produced biofilm on Polystyrene microtiter plates. Results revealed that all drinking water isolates produced biofilm (18 isolates )and 42 raw water isolates produced biofilm (depending on Polystyrene microtiter plates test). The more two efficient isolates(one isolated from drinking water DW, and other isolated from raw watervRW) which produced biofilm was chosen to study the effect of different values of temperature and disinfectants on the ability of A.hydrophila to ad

Response surface methodology (RSM) based on central composite design was successfully applied to redesign MRS media for maximizing both biomass and bacteriocin production from Lactobacillus plantarum NH40. First, glucose and yeast extract were chosen as the best carbon and nitrogen sources based on classical optimization results of one factor at time which also revealed the possibility of eliminating peptone and meat extract from the original composition of medium without affecting the growth and bacteriocin production. Statistical experimental design based on a regression model generated using the Design expert 7 software showed that the optimum concentrations of glucose, yeast extract, tween80, NH₄Cr, CH

Abstract :- In this paper, silver nanoparticles had been prepared by chemical reduction method. Many tests had been done to it such as UV-Visible spectrophotometer, XRD, AFM&SEM test. finally an attempt had been done to get the optimum condition to control the grain size of silver Nanoparticles by variation the heating period and other parameters which has an effect in silver Nanoparticles synthesis process. in this method we can get a silver nanoparticles in the size range from 52 to 97 nm.

Hydrophobic silica aerogels were successfully preparation by an ambient pressure drying method from sodium silicate (Na2SiO3) with different pH values (5, 6, 7, 8, 9 and 10). In this study, acidic HCl (1M), a basic NH4OH (1M) were selected as a catalyst to perform the surface modification in a TMCS (trimethylchlorosilane) solution. The surface chemical modification of the aerogels was assured by the Fourier transform infrared (FTIR) spectroscopic studies. Other physical properties, such as pore volume and pore size and specific surface area were determined by Brunauer-Emmett- Teller (BET) method. The effect of pH values on the bulk density of aerogel. The sol–gel parameter pH value in the sol, have marked effects on the physical proper

Two grades of paving asphalt with penetration of 46 and 65 are studied for determining changes in their physical and chemical properties caused by ageing.
The ageing process has been conducted on two petroleum paving asphalt cement using thin film oven test at 150, 163 and 175 C, and ageing time 5, 10,15, 20, 25 and 30 hours. The effect of ageing time and temperature on penetration, kinematic viscosity, softening point, solubility in trichloroethylene, heat loss and changes in chemical composition are investigated. The results of thin film oven test process indicte that the asphaltenes concentration of all aged asphalt increases with increasing ageing time, while the opposite was observed for polar-aromatic and naphthene-aromatic. The