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Determination of Antibodies (IgG, IgM) against Toxoplasma gondii in Some Iraqi individuals by using ELISA technique

A total of 258 voluntary blood donors (males 101; females 157) in the age range of 18-52 yr among males and 18-55 yr among females were examined for Toxoplasma gondii antibodies (IgG), and (IgM) by immunological technique (Enzyme linked Immunosorbant Assay) during the period from March 2009 to April 2010. This study covered a wide range of factors including immunological, age ,sex , place of residence and symptoms that may have a possible relationship with toxoplasmosis. Results presented in this study showed clearly that 38 (14.7%) of individuals participated in this study having IgG Toxoplasma Ab, among those 10 samples (9.9%) were males and 28 samples (17.8%) were females. Moreover, we found the prevalence of IgM seropositivity in the study population to be 5.8% ,as well as, the prevalence of IgM was 1.98 % in males and 8.3% in females. In addition to , the results of current study indicated that the seroprevalence of IgG Toxoplasma antibodies are more than IgM antibodies ,besides , the peak period range of toxoplasma gondii antibodies IgG among males donors was 30 to 39 years, while among female donors, the highest detection of Toxoplasma gondii antibodies IgG was between the ages of 40 to 49. What's more, the peak age range of Toxoplasma gondii antibodies IgM among males and females donors was 19 to 29 years. In conclusion , Our study showed a high prevalence of T. gondii antibodies in healthy voluntary blood donors. It may be appropriate to include screening test (ELISA) for T. gondii also in the pre transfusion blood testing schedule.

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Publication Date
Sun Apr 29 2018
Journal Name
Iraqi Journal Of Science
Estimation the Missing Data of Meteorological Variables In Different Iraqi Cities By using ARIMA Model

In this paper, ARIMA model was used for Estimating the missing data(air temperature, relative humidity, wind speed) for mean monthly variables in different time series at three stations (Sinjar, Baghdad , AL.Hai) which represented different parts of Iraq from north to south respectively

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Publication Date
Sun Jul 09 2023
Journal Name
Journal Of Engineering
Increasing of Naphthenes Content in Naphtha by Using Y and  Zeolite Prepared from Iraqi Kaolin

This work dealt with separation of naphthenic hydrocarbons from non-naphthenic hydrocarbons and in particular concerns an improved process for increasing the naphthenes concentration in naphtha, The separation was examined using adsorption by Y and  B zeolite in a fixed bed process. The concentration of naphthenes in the influent and effluent streams was determined using PONA classification. The effect of different operating variables such as feed flow rate (2- 4 L/hr); bed length (50 - 80 cm) on the adsorption capacity of Y and  zeolite was studied. Increasing the bed length lead to increase the naphthenes concentration, and increasing the flow rate lead to decrease in the concentration of naphthenes, It was found that the decrease

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Publication Date
Mon Sep 16 2019
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Determination of Ibuprofen in Pharmaceutical Formulations Using Differential Pulse Polarography

     A reliable differential pulse polarographic (DPP) method has been developed and applied for the determination of ibuprofen IBU in dosage form with dropping mercury electrode (DME) versus Ag/AgCl. The best peak was found at cathodic peak of -1.18 V in phosphate buffer at pH=4 and 0.025M of KNO3 as supporting electrolyte. In order to obtaine the highest sensitivity, instrumental and experimental parameters were examined including the type and concentration of supporting electrolyte, pH of buffer solution, pulse amplitude and voltage step time. Diffusion current showed a direct linear relationship to ibuprofen concentration in the range of (5 – 30) μg. mL-1 (2.43× 10-5

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Publication Date
Mon Jan 01 2018
Journal Name
Journal Of Global Pharma Technology
Auto-antibodies Profile in Children Infected with Visceral Leishmaniasis

Visceral leishmaniasis (VL) is a parasitic infection caused by an intracellular growth of Leishmania spp. in macrophage cells. The autoimmune disorder is a condition takes place when the immune system produces antibodies which incorrectly attacked its own body tissues. VL has been involved as an effect or on the autoimmune aspect. This study was conducted to identify the auto antibodies profile in patients infected with VL. The presences of auto antibodies in 21 Iraqi children infected with VL were tested for laboratory autoimmune aspect. The highest percentage of seropositive in Leishmania patients was observed for anti-ds DNA, anti-Mi-2, anti-Ku and anti-PCNA antibodies (90.5%, 90.5%, 90.5% and 61.9%) respectively, while the lowest percen

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Publication Date
Fri Dec 01 2023
Journal Name
Iranian Journal Of Medical Microbiology
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Publication Date
Mon Mar 08 2021
Journal Name
Baghdad Science Journal
Brucella antibod titres base line in the healthy individuals in haweja city

152 sera were collected from healthy individuals residing A;-Haweja City were tested for antibody titers for brucella antigens by slide agglutination test

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Publication Date
Fri Jan 01 2016
Journal Name
World Journal Of Experimental Biosciences
Detection of anti-Helicobacter pylori antibodies in sera of women with recurrent spontaneous abortion

To determine the relationship between Helicobacter pylori infection and reproduction disorder (recurrent spontaneous abortion), twenty women patients who undergo spontaneous abortion during first trimester of pregnancy (20-38) years and have been investigated from 2015/12/1 -2016/3/1 and compared to fifteen healthy individuals. All subjects were carried out to measure anti-H. pylori IgA and anti- H. pylori IgG antibodies by enzyme linked immunosorbent assay (ELISA). There was significant elevation (p≤ 0.05) in concentration of anti- H. pylori IgG Abs (6.30± 0.99) compared to control group (4.48± 0.61) and IgA Abs (5.42 ± 0.90 U /ml) as compared to control group (3.92 ± 0.41 U/ml). The percentage of H. pylori IgG and IgA was 20% and 25

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Publication Date
Sun Jun 05 2011
Journal Name
Baghdad Science Journal
Quantitative Analysis of Some Insecticides and Their Mixture in Agriculture by Using UV-Vis Spectrophotometer

We studied in this research how to find a method of estimating the quantity (Kinetically) of three kinds of Insecticide and their mixture, which are used in agriculture. The extracted insecticide from the polluted samples with these insect from air, soil, and the leaves of trees, have be used into the reaction with H2O2 and benzedine. The kinetic study of this reaction was formed in basic medium,( pH= 8.6), using UV. Spectra at (?= 420nm). The study showed that the reaction is the first order, and the speed of the reaction was used to estimate the concentration of insecticide in solution and mixture. The experiments of this study indicated that this method has the speed and efficiency for quantitatively estimating these

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Publication Date
Sun Sep 29 2019
Journal Name
Iraqi Journal Of Science
Determination of the Critical Period of Cyper-diforce® Treatment against Arthropod fauna and Productivity of Watermelon

     Watermelon has been reported to be vulnerable to insect pest pressure throughout its growth stages with a resultant indiscriminate calendar-based insecticide application (of up to 25 sprays/season in the study area), with its attendant consequences. In order to recommend the crop growth period(s) to effect chemical control measures that will give better return on investment, field trials were set-up in the early- and late-cropping seasons of 2016 and 2017. Forty, 5m long x 8m wide plots were demarcated in randomized complete block design in 4 replications. Treatments were applications of 0.5% Cyper-diforce® (Cypermethrin 30g/L + Dimethoate 250g/L) at seedling, mid-vegetative, mid-flowering, mid-frui

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Publication Date
Mon Aug 07 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Study on The Effects of Boron on The Structure of Chloroplasts of Cauliflower , By Using Light Scattering Technique

         Effects of Boron on the structure of chloroplasts membrane isolated from cauliflower are investigated , using light scattering technique. Results obtained in this study suggest that Boron in the concentration range (0.1-5 µm) can fluidize the lipids of the chloroplast membrane due to different extent. Mechanisms by which Boron can change the lipid fluidity is discussed. Furthermore, an experimental evidence is presented to show that2µM Boron can mediate conformational changes in the membrane –bound proteins of the cauliflower’s chloroplast.

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