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Determination of Antibodies (IgG, IgM) against Toxoplasma gondii in Some Iraqi individuals by using ELISA technique

A total of 258 voluntary blood donors (males 101; females 157) in the age range of 18-52 yr among males and 18-55 yr among females were examined for Toxoplasma gondii antibodies (IgG), and (IgM) by immunological technique (Enzyme linked Immunosorbant Assay) during the period from March 2009 to April 2010. This study covered a wide range of factors including immunological, age ,sex , place of residence and symptoms that may have a possible relationship with toxoplasmosis. Results presented in this study showed clearly that 38 (14.7%) of individuals participated in this study having IgG Toxoplasma Ab, among those 10 samples (9.9%) were males and 28 samples (17.8%) were females. Moreover, we found the prevalence of IgM seropositivity in the study population to be 5.8% ,as well as, the prevalence of IgM was 1.98 % in males and 8.3% in females. In addition to , the results of current study indicated that the seroprevalence of IgG Toxoplasma antibodies are more than IgM antibodies ,besides , the peak period range of toxoplasma gondii antibodies IgG among males donors was 30 to 39 years, while among female donors, the highest detection of Toxoplasma gondii antibodies IgG was between the ages of 40 to 49. What's more, the peak age range of Toxoplasma gondii antibodies IgM among males and females donors was 19 to 29 years. In conclusion , Our study showed a high prevalence of T. gondii antibodies in healthy voluntary blood donors. It may be appropriate to include screening test (ELISA) for T. gondii also in the pre transfusion blood testing schedule.

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Publication Date
Fri Jun 24 2022
Journal Name
Iraqi Journal Of Science
Local Study of blaCTX-M genes detection in Proteus spp. by using PCR technique

In this study, 25 clinical isolates of Proteus spp. were collected from urine, wounds and burns specimens from different hospitals in Baghdad city, all isolates were identified by using different bacteriological media, biochemical assays and Vitek-2 system. It was found that 15 (60%) isolates were identifies as Proteus mirabilis and 10 (40 %) isolates were Proteus vulgaris. The susceptibility of P. mirabilis and P. vulgaris isolates towards cefotaxime was (66.6 %) and (44.4 %) respectively; while the susceptibility of P. mirabilis and P. vulgaris isolates towards ceftazidime was (20%). Extended spectrum β-lactamses producing Proteus was (30.7 %). DNA of 10 isolates of P. mirabilis and 4 isolates of P. vulgaris were extracted and detecti

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Publication Date
Thu Dec 28 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Determining the Concentrations of Elements in Tobacco Selected in Iraqi Markets Using X-Ray Fluorescence Technique

  X-rays fluorescence technology was used  to measure the concentrations of trace and toxic elements in tobacco smoke. One sample local and eight samples were imported selected from Iraqi markets. The results proved that tobaccos contain few concentrations of element (calcium, potassium, sodium, manganese, magnesium, chlorine, and sulfur), trace concentrations of element (aluminum, vanadium, iron, cobalt, nickel, copper and zinc), and toxic concentrations of element (arsenic, selenium, bromine, antimony, cadmium, mercury and lead). Results are proved the concentrations of elements of samples are lower levels with the other countries in few element like (calcium, potassium, sodium and manganese), while the other elements were hi

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Publication Date
Tue Jan 18 2022
Journal Name
Special Care In Dentistry
Association between self‐reported oral disease/conditions and symptoms of depression among Iraqi individuals
Abstract<sec><title>Aims

The negative impact of oral diseases on the function, economy, and general health of the population is well‐documented. In the last decades, evidence linking increased expression of depression and oral diseases/conditions has significantly increased. The aim of this study is to assess the association between oral disease/conditions and self‐reported symptoms of depression individuals.

Methods

A specially designed questionnaire was distributed via social media for 1 week. It consisted of two main sections; the first section was dedicated to collect demographic variables and self‐reported symptoms

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Publication Date
Tue Nov 19 2024
Journal Name
Journal Of Stomatology
Association of modifiable and non-modifiable risk factors with periodontal disease in Iraqi individuals: a retrospective study

Introduction Periodontal diseases are ranked among the most common health problems affecting mankind. These conditions are initiated by bacterial biofilm, which is further modulated by several risk factors. Objectives To investigate the association of different risk factors with periodontal...

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Publication Date
Wed Oct 31 2018
Journal Name
Iraqi Journal Of Science
Determination the Concentration Elements of Cultivation Media (Peat Moss, Perlite and Hormone) Using X-ray Fluorescence Technique

The concentration of elements were analyzed of twelve cultivation medium (Peat moss, Perlite and Hermon) selected from Iraqi markets using X-ray fluorescence techniques. The analytical results show that the cultivation medium contained high concentration of (Na, Al, Si, S, K, Ca, Fe) and low concentration of (Mg, P, Cl, Ti, V, Cr, Mn, Co, Ni, Cu, Zn). The samples also contained trace concentration of (Ge, As, Se, Br, Sr, Y, Mo, Cd, I, Hg, Pb, U). The results were compared using atomic absorption spectrophotometric technique for measuring the concentration of (K, Ca, Cu, Mn, Zn, Pb).

The Results showed that there is significant difference in the concentration of each element in most of the samples. The concentrations of elements a

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Publication Date
Mon Jan 30 2023
Journal Name
Iraqi Journal Of Science
Diagnostic Efficiency of Heat Shocked Protoscoleces Extract Antigens for Human Cystic Echinococcosis by ELISA

      Diagnosis of cystic echinococcosis is complex and has to be confirmed by the combination of immunological tests and imaging techniques. In this study heat shock proteins were induced and their immunoreactivity was assessed by ELISA.

Sera were collected from 34 hydatid patients who were admitted to the Rizgary Teaching Hospital through October 2013 to July 2017, in addition to 29 healthy donors and 18 non-hydatid cases. For heat shock response, two batches of 25000 protoscoleces (Ps) were incubated separately at 42°C and 45 °C for 4 hours. Heat treated and normal Ps were disrupted and the extracts were divided into two parts. One part was directly used as source of antigens (PE, PE42 and PE45) and the other one was par

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Publication Date
Wed Aug 09 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Vaccination of chicken against Coccidiosis by using live Oocysts of different species of Eimeria

The study was conducted to vaccinate chickens against coccidiosis  using alive vaccine contain seven species of chicken Eimeria,  E. tenella, E. brunetti, E. necatrix, E. maxima, E. mevati, E. acervulina  and E. praecox.    A total of 120 chicks were divided into two main groups: broiler group and egg laying group.  The birds of each group were allocated on to three pins, each contains 20 chicks.  The birds of the experiment were vaccinated at day nine of age, with a suspension of mixed Eimeria which contain 50 Oocysts of E. tenella with a different percent of other species, respectively.  The vaccine was given to chicks in the 1st pins of each group with drinking water,  the chicks in th

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Publication Date
Fri Dec 30 2022
Journal Name
Iraqi Journal Of Science
Detection of FOXP-3 Expression in a Sample of Iraqi Cervical Cancer Patients Using Immunohistochemistry Technique

     This paper aims to find out if FOXP-3 was expressed in samples from Iraqi cervical cancer patients. Expression of FOXP-3 was detected in 55 cervical tissue samples by immunohistochemistry. Since thirty-five cases of aggressive cervical cancer were included, along with 20 normal samples used as controls. The nucleus and cytoplasm levels of FOXP-3 were counted, considering the ratio of positive cells and intensity. FOXP3 cytoplasmic staining was found in 27 out of 35 cases. Only 11 out of 35 samples displayed nuclear lymphocyte staining. Furthermore, four samples expressed this marker in both the nuclear and cytoplasm of the cervical cells. There is a highly significant difference in FOXP3 expression in the cytoplasm of

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Publication Date
Mon Oct 03 2016
Journal Name
Applied Research Journal
DETERMINATION OF THE GEOID HEIGHT (GEOID UNDULATION) BY USING MODERN SURVEYING TECHNOLOGIES

In this work, a large part of Baghdad University campus has been selected. The determination of Geoidal height for the local area requires Ground Control Points which both Ellipsoidal and Orthometric heights are known to compute the difference between them. The first step of the leveling process began by selected the Ground Control Points (GCPs) around the area of the work, and then divided them into two groups of the network traverse stations. They were leveled and adjusted depend on the number of the Bench Marks (B.M.s). Total Station TS (Nikon Nivo 5C) and Global Positioning System (GPS-Garmin 78 map) are used to do this application. The aim of the proposed work was to determine the height of the Geoid surface in the study area. The Geoi

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Publication Date
Wed Feb 22 2023
Journal Name
Iraqi Journal Of Science
Determination of Destruction Levels Caused by Earthquakes Using Grip RADIUS 99 Program

The most universal and basic damages caused by an earthquakes are buildings damage and human casualties. A simplified method, the RADIUS 99 Tool is used to calculate seismic intensity (shaking) distribution, buildings damage, number of casualties and lifelines damage, due to assumed earthquake scenario. In this study, Al - Kadhmiya sector in Baghdad city was chosen for assessing seismic risk, for this purpose, this area was divided into mesh of 1*1 km2 cell size, and a scenario of (Manjil) earthquake (that struck Iran in 1990) was utilized with following earthquake magnitudes (5 and 7), with epicenter distance (3, 10 and 100 km), and depths (2 and 5 km). It was found that, the best soil types for constructions are those with medium and h

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