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Effects of Allopurinol on Ketone Body Metabolism and Tissue Lipid Peroxidation in Alloxan Diabetes in Rats

The aim of the present study is to investigate whether or not xanthine oxidase (XO)–derived reactive oxygen species (ROS) may play a role in the pathogenesis of alloxan (ALX)–induced diabetes in rats using the specific XO inhibitor and hydroxyl radical scavenger, allopurinol

The involvement of oxidative stress in ALX – diabetes was assessed by the measurement of plasma and various tissues lipid peroxides levels ( using thiobarbituric acid ( TBA ) reactive substances ). Furthermore, the ability of allopurinol to influence these and other biochemical parameters, including plasma and urine ketones levels were also investigated in diabetic rats.

Rats were divided into four groups: control, untreated diabetic, allopurinol – treated diabetic, and insulin – treated diabetics. At the end of the one week experimental period, blood and tissue samples were obtained from anesthesized animals for the measurement of the above – mentioned parameters.

   Although the single intraperitoneal (i.p.) injection of allopurinol (25 mg/kg body wt.) 1h before or 1h after ALX injection (100 mg/kg body wt., i.p.) failed to prevent the induction of diabetes, it did lower ketonuria and the incidence of early ketosis–associated mortality in diabetic animals in comparison with non–allopurinol–treated diabetic rats. Subsequent administration of allopurinol (25 mg/kg body wt., i.p.) every 48 hr for 1wk (i.e., 3 additional doses) also decreased plasma ketone bodies levels as well as plasma and tissue (heart, liver, kidney, pancreas) lipid peroxides levels in comparison with non–allopurinol–treated diabetic rats. Daily insulin injection (9–12 U/kg body wt., S.C.) for 1wk period normalized all of the above–mentioned abnormalities.

The present results suggest that XO–derived ROS play a minor role (if any) in the diabetogenic effect of ALX. On the other hand, although the mechanism (s) underlying the protective effects of allopurinol on the diabetic state is presently unknown, these effects may reflect a possible association between impaired ketone body metabolism and lipid peroxidation: and suggest an effect of allopurinol on ketone body metabolism.

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Publication Date
Wed Jul 01 2009
Journal Name
Journal Of The Faculty Of Medicine Baghdad
The In Situ Hybridization Expression of Fas and Fas Ligand (FasL) in Trophoblastic tissue of Aborted Women Compared with Normal Pregnancy.

Background: Estimation of the in situ expression of Fas and Fas ligand (FasL) in aborted women compared with normal human pregnancy .
Patients and Methods: A technique utilizing in situ hybridization(ISH) was performed to detect and determine the in situ expression of Fas and Fas ligand (FasL) mRNA using paraffin embedded sections of curettage samples obtained from 42 women, who were divided into two groups: 30 women with first trimester abortion and 12 women with induced abortion as control.
Results: The levels of the in situ expression of both Fas and Fas ligand (FasL) mRNA were found to be highly significant increased in group 1 as compared with group 2
(p<0.01), with a significant positive correla

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Publication Date
Sun Jul 21 2024
Journal Name
Cureus
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Publication Date
Tue Oct 26 2021
Journal Name
Iraqi Journal Of Science
Theoretical Study for the Calculation of Proton Range in Human Body Tissues

     The main rationale for using charged particles in radiation therapy is the strong rise of energy loss (deposited dose) with maximum penetration depth ( Bragg peak) and rapid dose deposited  behind the peak. Thus, a large dose can be  applied to a deep seated tumor, with saving the surrounding normal tissues . Proton radiotherapy is nowadays an established method in the management of cancer diseases, although its availability is still limited to a few specialized centers. In this study, the range and the stopping power for proton interaction  in the skeleton  and intestine tissues, for an energy range from 0.01 to 300 MeV, was studied. The numerical calculations and analyses of Bethe&nbs

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Publication Date
Fri May 07 2021
Journal Name
Pakistan Journal Of Medical Sciences
Treatment options of Adolescent Gestational Diabetes: Effect on Outcome

Objectives: Teenage pregnancy with gestational diabetes mellitus (GDM) offers a real challenge to the health system and needs a special care. We aimed to evaluate possible obstetrical and neonatal adverse events of different treatment protocols in adolescent GDM including lifestyle, metformin (MTF), and insulin. Methods: All teen pregnant women ≤ 19 years old visiting Baghdad Teaching Hospital throughout four years (from June 1, 2016 till May 31, 2020) diagnosed with GDM were included in this cohort study and followed-up closely throughout pregnancy and after delivery. Included adolescents were put on lifestyle alone during the first week of presentation. Adolescents who reached target glucose measurements were categorized i

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Publication Date
Sun Oct 02 2011
Journal Name
Journal Of The Faculty Of Medicine Baghdad
In Vitro Study of Mutagenicity and Genotoxicity of Some Bacterial Secretions associated with Bladder Tumors Tissues on Human Lymphocytes by Using Blood Tissue Culture Techniques

Background: Cytogenetic studies have been used to assess carcinogenic or mutagenic exposures and effects early in occupational, biological and environmental settings. Bacterial infection has not traditionally been considered asa major causes of cancer. Bacteria have been linked to cancer by two mechanisms: induction of chronic inflammation and production of carcinogenic bacterial metabolites.
Objective: Because of new concepts in relating infection with certain malignancies the present study is performed. Therefore this study was conducted to determine the effects of bacterial products associated with biopsies of bladder tumor on human lymphocytes tissue cultures in vitro. Moreover, if there is any relation between these products for

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Publication Date
Wed Sep 18 2024
Journal Name
World Academy Of Sciences Journal
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Publication Date
Thu Apr 01 2021
Journal Name
Biochem. Cell. Arch.
AGE AND GENDER IMPACT ON GLYCAEMIC CONTROL, RENAL FUNCTION AND OXIDATIVE STRESS PARAMETERS IN IRAQI PATIENTS TYPE 2 DIABETES MELLITUS

Type 2 daibetes mellitus (T2DM) is a global concern boosted by both population growth and ageing, the majority of affected people are aged between (40- 59 year). The objective of this research was to estimate the impact of age and gender on glycaemic control parameters: Fasting blood glucose (FBC), glycated hemoglobin (HbA1C), insulin, insulin resistance (IR) and insulin sensitivity (IS), renal function parameters: urea, creatinine and oxidative stress parameters: total antioxidant capacity (TAC) and reactive oxygen species (ROS). Eighty-one random samples of T2DM patients (35 men and 46 women) were included in this study, their average age was 52.75±9.63 year. Current study found that FBG, HbA1C and IR were highly significant (P<0.01) inc

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Publication Date
Wed Oct 31 2018
Journal Name
Iraqi Journal Of Science
Effects of some physical agents on Staphylococcus epidermidis and Leishmania tropica: An In vitro study

This work evaluated the effect of Alpha, Gamma  irradiation and Nd:YAG,  He-Ne laser on  Staphylococcus epidermidis and  Leishmania tropica  in vitro. The experiment included five replicate  of   S. epidermidis , L. tropica  in vitro exposed to effect of Alpha , Gamma  irradiation by 241Am  isotopes , in two  doses

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Publication Date
Sun Jun 02 2013
Journal Name
Baghdad Science Journal
Effects of Lead on Hemopoeitic and Iron Status in Iraqi Workers at Lead Batteries Factory

The present study aimed to evaluate the effect of lead exposure on hemopoetic system (through the index delta-Aminolevulinic acid dehydratase ?-ALAD activity & hemoglobin concentration (Hb) ) and on iron status (levels of iron Fe, Ferritin Fr, Total iron binding capacity TIBC, percentage of transferine saturation TF%) in 44 Iraqi worker at lead batteries factory. Workers divided into two groups: smokers(n=21) mean aged (37.33±4.82 year)and non smokers(n=23) mean aged(40.78±7.89 year) and 45 healthy subjects mean aged (33.97±5.08)as control group . Activity of ?-ALAD ratio shows significant decrease (p ? 0.05) ,while Hb and hematocrit Hct were non significant (p ? 0.05) in smoker workers more than non smoker as compared to control . The r

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Publication Date
Tue Jun 12 2007
Journal Name
Iraqi Journal Of Laser
Incident Laser Power and Concentration Effects on the Fluorescence of DCM Dye in PMMA Polymer

Incident laser power and concentration effects on fluorescence emission from DCM dye in PMMA polymer have been investigated. Different concentrations of the dye were used. It was found that the fluorescence intensity increased with increasing of the concentration of the dye, with a red shift. In addition, it was found that the fluorescence intensity increased with the increase of the incident laser power I0.

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