1. baumannii is an aerobic gram negative coccobacilli, it is considered multidrug resistance pathogen (MDR) and causes several infections that are difficult to treat. This study is aims to employ physical methods in sterilization and inactivation of A. baumannii, as an alternative way to reduce the using of drugs and antibiotics.

            Cold Atmospheric Plasma was generated by one electrode at 20KV, 4 power supply and distance between electrode and sample was fixed on 1mm. A. baumannii (ATCC 19704 and HHR1) were exposed to  Dielectric Barrier Discharge type of Cold Atmospheric Plasma (DBD-CAP) for several periods

Acinetobacter baumannii is highly adapted to hospital environments, causing persistent chronic infections due to its ability to form biofilms. In this work, the antibiofilm activity of AuNPs with a subMIC concentration of 9.34 μg/ml was investigated by the microtiter plate method against 80 clinical isolates of A. baumannii. The results revealed that the biofilm was significantly (P< 0.05) reduced by 48.2 – 82.1%.

Background: Acinetobacter baumannii is a significant opportunistic pathogen and it is generally associated with benign colonization of hospitalized patients.

Objective: To investigate skin colonizationwith Acinetobacter baumannii in hospitalized patients and healthy volunteers.Antimicrobial resistance patterns of Acinetobacter baumanniiwas assessed by determining the minimum inhibitory concentrations (MICs) of thirteen different antimicrobial agents.

Patients and Methods: The study performed on hospitalized patients at Rizgary and Hawler teaching hospitals and healthy volunteers who attended to supermarkets in Erbil, Iraq. A single sample was ob

This study was aimed to analysis phylogenetic tree of the gene cpn60 in Acinetobacter baumannii that was identified in Baghdad. Study included collection two hundred specimens (fifty from UTI, fifty from wound infection , fifty from respiratory tract infection and fifty from otitis infections) . In primary laboratory diagnosis and confirmed by using VITEK- 2 Compact system, twenty isolates of this bacterium were indentified (10%) from total specimens. Extraction of geneteic material to detect target gene by amplification this target gene. DNA
sequencing of all isolates was done. Then alignment of sequencing in NCBI and draw phylogenetic tree by use Geneious 9 software among sequence of locally i

Siderophores are low molecular weight organic compounds produced by microorganisms growing under low iron concentration.In this study we describe the detection, production and extraction of siderophores secreted by Acinetobacter baumannii (Multiple-drug resistant ) pathogens. One hundered twenty Gram –negative non lactose fermenter bacilli isolates have been collected from three hospitals at Baghdad city over three months. Primary identification of these isolates is performed by standard diagnostic methods (biochemical tests and API 20 NE); 19 clinical isolates of A. baumannii are cultured on CHROMagar (highly selective medium for detection of MDR Acinetobacter) as well as diagnoses is documented by using Vitek 2 system. Isolates are exa

The current study focuses on the bacterium Acinetobacter baumannii due to its importance as a nosocomial infections source in addition to its increased resistance against antibiotics. Different clinical and hospital environment samples were collected, and cultured on A. baumannii selective media: Leed Acinetobacter agar and Herellea agar. A. baumannii have been identified by traditional methods, followed by confirmation using molecular identification to detect bla_{oxa-51 like} gene which is considered a diagnostic gene since it is present in genome of all A. baumannii strains. The result was, nineteen bacterial isolates of A.baumannii were obtained, from twenty-seven suspected isolate

A total of 100 clinical sample from (urine, sputum and swabs of wound , burn and ear) were collected from patients in different hospitals of Baghdad during the period from December 2013 to May 2014. 15 isolates (15%) identified belong to Acinetobacter baumannii, swabs of wounds were represented in high percentage of A.baumannii isolates (40%) while percentage of other samples were variable. Susceptibility of 15 A.baumannii isolates were tested toward 16 different Antimicrobial agents, the results showed all isolates were multi drug resistant. In addition, Polymerase Chain Reaction Technique (PCR) was performed to detection the resistance genes encoding the Oxacillinases enzymes. The PCR analysis showed that the presence of insertion sequ

The isolates of Staphylococcus aureus were isolated from patients with various infections in hospitals, the isolates were identified and accurately diagnosed by phenotypic examination and biochemical tests, as well Vitek-2, and then genetic detection and diagnosis of many of the pathogenic factors associated with Staphylococcus aureus using conventional polymerase chain reaction (PCR) and testing for association by antibiotic resistance and production of some toxins by Staphylococcus aureus. After performing analysis of statistical, it was set up that the correlation coefficient of the PCR technique using virulence genes, sensitivity test to antibiotics and other virulence factors were significant at p < 0.05, but was insignificant with the

     Acinetobacter baumannii has been recently classified as a major threat to public health because it has resistant to almost all antibiotics and there are many reasons that are responsible for conferring this feature to A. baumannii. One of these reasons is integrons so in this study we show the role of the integrons in providing resistance to some antibiotics. A number of 60 isolates were collected from different clinical sources of patients who were admitted to Baghdad hospitals and all isolates were diagnosed using biochemical tests and confirmed using  Chrom-ager culture media, and Vitek 2 compact system. The antibiotic susceptibility test was determined during this study using Kirby-Bauer method and the res