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Design, Synthesis, Characterization and Preliminary Anticancer Study for Methotrexate Silibinin Conjugates

The spectrum of clinical efficacy of Methotrexate (MTX) is broad in that MTX is used in the treatment of certain cancers, severe psoriasis and rheumatoid arthritis.Various mechanisms by which cancer cells grown in tissue culture become resistant to anticancer drugs. The use of multiple  drugs with different mechanisms of entry into cells and different cellular targets allows for effective chemotherapy and high cure rates. In an efforts to develop effective strategies that increase the therapeutic potential of anticancer drugs with less systemic toxicity ,are being directed  towards the investigation of dietary supplements and other phytotherapeutic agents for their synergistic efficacy in combination with anticancer drugs. A promising approach to improve the cancer cell selectivity of methotrexate is the chemical transformation into reversible derivatives which convert the conjugate to the parent drug by virtue of enzyme within cancer tissue. The present study includes the synthesis of  two derivatives of methotrexate which are :-Schiff base methotrexate-silibinin conjugate ( compound 5), and Methotrexate-silibinin conjugate (compound 6).The synthesis  of the target compounds was  accomplished following multistep reaction procedures. The chemical reactions were followed up and purity of the products was checked by TLC. The structures of the final compounds and their intermediates were characterized and identified by their melting points, infrared spectroscopy, 1H-NMR and elemental microanalysis(C H N S).The anticancer activity of these compounds was investigated by HEP-2 cell line(Larynx carcinoma), which showed  that compounds 5 and 6 have the higher activity than methotrexate or silibinin alone.These are promising data for the discovery of new anticancer agents in future. These compounds may deliver the parent drug  selectively  into the cancer cells to be hydrolyzed by enzymes that are elevated in  tumor tissues compared with normal tissues .

Keywords: Methotrexate, Silibinin, Cancer treatment resistance, Folate receptor, Cancer cell targeting.

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Publication Date
Sun Aug 06 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Approximation Solutions for System of Linear Fredhom Integral Equations by Using Decomposition Method

In this paper, the Decomposition method was used to find approximation solutions for a system of linear Fredholm integral equations of the second kind. In this method the solution of a functional equations is considered as the sum of an infinite series usually converging to the solution, and Adomian decomposition method for solving linear and nonlinear integral equations. Finally, numerical examples are prepared to illustrate these considerations.

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Publication Date
Thu Apr 28 2022
Journal Name
Iraqi Journal Of Science
Distinction of Two Zero-Spaced Iron Pipes in GPR Radargram for Engineering Problems

The researchers have tried to focus on how to determine the number of pipes that are present in one obtained hyperbola in radargram profile. Ground Penetration Radar (GPR) survey was performed to distinguish between two zero-spaced iron pipes in radargram. The field work was carried out by constructing artificial rectangular models with dimensions of length, width, and depth equal to 10.0, 1.0, 0.65 meter respectively that filled with dry clastic mixture deposit, three twin sets of air filled iron pipes of 15.24 cm (6 inch) diameter were buried horizontally and vertically inside the mixture at different distances together. Visual and Numerical interpretation were chosen to get the best results. In the visual interpretation, the amplitude

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Publication Date
Sat Nov 03 2018
Journal Name
Iraqi National Journal Of Nursing Specialties
Evaluation of Nursing Care Management for Diabetes Mellitus Patient with Total Hip Replacement

Objective: The aim of the study to evaluate the nursing care management for diabetes mellitus patient
with total hip replacement after fractured hip.
Methodology: A field study carried out on patients with diabetes mellitus and have total hip
replacement after fractured hip in orthopedic ward at the hospital of surgical specialization (malefemale)during
January 2002 to January 2003.Physical and psychological nursing
assessment
immediately after the surgery was done for the both subjects (control and experimental) and then a
scientific management with daily nursing care were provided to the experimental subject with daily
nursing care to the patient condition by using a scientific and practical methods and leave th

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Publication Date
Fri Jun 24 2022
Journal Name
Iraqi Journal Of Science
Water Balance for Ali AL-Gharbi-Northeast of Missan Governorate- Southeast of Iraq

Ali AL-Gharbi area lies to the northeast of Missan Governorate, southeast of Iraq. The meteorological data recorded in Ali AL-Gharbi station for the period (1994-2014) were used to assess the climatic condition of the study area, it was found that the monthly mean of rainfall is (15.35 mm), relative humidity (43.95 %), the temperature (24.50 C◦), wind speed (4.35 m/sec) and the strongest and most frequent winds are the northwest, sunshine (8.54 h/day) and evaporation (305.73 mm).The results of the data analysis show that, the climate of study area is characterized by dry and relatively hot in summer, and cold with low rain in winter. This study shows that, there is water surplus of (35.69 %) of the total rainfall amount which is equivalen

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Publication Date
Sat Aug 01 2015
Journal Name
Journal Of Engineering
Analytical Approach for Load Capacity of Large Diameter Bored Piles Using Field Data

An analytical approach based on field data was used to determine the strength capacity of large diameter bored type piles. Also the deformations and settlements were evaluated for both vertical and lateral loadings. The analytical predictions are compared to field data obtained from a proto-type test pile used at Tharthar –Tigris canal Bridge. They were found to be with acceptable agreement of 12% deviation.

               Following ASTM standards D1143M-07e1,2010, a test schedule of five loading cycles were proposed for vertical loads and series of cyclic loads to simulate horizontal loading .The load test results and analytical data of 1.95

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Publication Date
Sun Nov 01 2009
Journal Name
Tencon 2009 - 2009 Ieee Region 10 Conference
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Publication Date
Fri Sep 01 2023
Journal Name
The Medical Journal Of Malaysia
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Publication Date
Sun Feb 10 2019
Journal Name
Journal Of The College Of Education For Women
Views syntactic grammar for Abi Sufyan (325 A.H) In his book Altvsh language

Arab heritage have teem much of the literature value, written by evacuated, who had a legacy of linguistic great scientists, but the circumstances are unknown, did not write those books or authors emerge, chose to search for a book of those compositions, he did not write to him and his book emerge, fell hands a book (Altvsh in the language) to Abu Sufyan grammar, a fourth-century scientists, as it is Abu Sufyan grammar of scientists who rolled up the time their impact, and the cryptic revelations among the stars, preferring to stand on this book and its author, a statement of the most important grammatical aspects contained therein; to notch the curtain for this world, and his book (Altvsh in strewn language and Mnzawmha and express the

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Publication Date
Wed Jul 01 2009
Journal Name
Journal Of The Faculty Of Medicine Baghdad
A New Method for DNA Isolation from Bone Marrow & Sperms of White Mice

Background: A quick and easy method was developed for extraction of DNA of eukaryotes from different samples, which are bone marrow and sperms in white mice Mus musculus strain (Balb/c).
Patients and Methods: this method using high salt buffer, Ethylene diemine tetracetec acid (EDTA), Trypsine,Sodium Dodecyl Sulfate(SDS), and urea without using Proteinase-K digestion or ultracentrifugation.
Results: This method was successful in extracting DNA from different samples in eukaryotic and this DNA is suitable for Hind III digestion.
Conclusion: Without further clean-up, the extracted DNA can be used for restriction endonuclease digestion or for numerous applications.

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Publication Date
Mon Sep 16 2019
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Strongly Convergence of Two Iterations For a Common Fixed Point with an Application

     In this paper, we study some cases of a common fixed point theorem for classes of firmly nonexpansive and generalized nonexpansive maps. In addition, we establish that the Picard-Mann iteration is faster than Noor iteration and we used Noor iteration to find the solution of delay differential equation.

 

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