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Detection of Epicatechin in Camellia sinensis Leaves by Thin Layer Chromatography and High Performance Liquid Chromatography Techniques

    The current study performed in order to detect and quantify epicatechin in two tea samples of Camellia sinensis (black and green tea) by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). Extraction of epicatechin from black and green tea was done by using two different methods: maceration (cold extraction method) and decoction (hot extraction method) involved using three different solvents which are  absolute ethanol, 50% aqueous ethanol and water  for both extraction methods using room temperature and direct heat respectively. Crude extracts of two tea samples that obtained from two methods were fractionated by using two solvents with different polarity (chloroform and ethyl acetate). Qualitative and quantitative determinations of epicatechin in tea samples were investigated. Epicatechin identification was made by utilizing preliminary chemical tests and TLC. This identification was also boosted by HPLC and the quantity of epicatechin was determined in all ethyl acetate fractions of two tea samples. This research revealed the existence of epicatechin in black and green tea according to TLC and HPLC. Aqueous ethanol 50% was the best solvent for extraction of epicatechin from leaves of tea. Quantitative estimation of epicatechin by HPLC revealed that ethyl acetate fraction of DGTAE contains the higher concentration of epicatechin than other analyzed fractions. Conclusion, tea is an excellent source of catechins particularly epicatechin that possessed various pharmacological effects.

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Publication Date
Tue Jan 29 2019
Journal Name
Journal Of Accounting And Financial Studies ( Jafs )
Implementing of Social Responsibility Standard ISO 26000 in Consumer Issues Market: Research and Consumer Protection Center -Study Case

Abstract The aim of this research is to show the grade of implementation of ISO 26000 (Social Responsibility Standard), specifically which related in clause sex (consumer issues), this study was achieved in Market Research and Consumer Protection Center (MRCPC) / University of Baghdad. The seven consumer issues of ISO 26000 was analyzed to show the extent of its implementation in MRCPC depending of using a check list as a principle instrument to collect research data and information. Results analysis was achieved by percentages and mean average. The research was leaded some of results and the most importance one was that the grade of implementation of the center in related to consumer issues given in the standard was medium

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Publication Date
Wed Aug 07 2019
Journal Name
Iraqi National Journal Of Nursing Specialties
Effectiveness of Post-Abortion Family Planning Counseling Program on Nurses and Midwives' Practices in Middle Euphrates Maternity Hospitals

Objectives: To determine the effectiveness of post-abortion family planning counseling program on nurses and midwives' practices and to predict the variables which may effect on their practices Methodology: A quasi experimental study was conducted from 23th April 2017 to 14th March 2018 in three governorates in Middle Euphrates of Iraq: (Holy Karbala, Al - Najef Al Ashraf and Babylon) on nurses and midwives who work at maternity hospitals. Systematic random sampling was used to select 122 nurses and midwives, (60) of them for study group and (62) for control group. A checklist is an instrument that evaluate the practices which included 50 items. Validity of content was determined through reviewing it by (16) experts and reliability of to

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Publication Date
Mon Feb 20 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Molecular Study to Detect Genotyping of Giardia lamblia from Human and Cattle Feces in Al-Qadisiya Governorate, Iraq

The present study is designed to diagnose the giardiasis from cattle and patients with diarrhea arrivals to Maternity and Childhood Teaching Hospital and General Education Hospital in Al-Qadisiya Governorate by using direct wet smear method as well as knowledge of the rate of prevalence of giardiasis in Al- Diwaniyah province, and study the effect of age, sex and nature of residence in the proportions of infection and investigate the genotypes of Giardia lamblia from human and animal feces ,100 samples were collected (50 stool samples of human and 50 feces samples of cattle). In human, the result showed that the infection rate was 54% (27). The age group of 2-4 years showed the highest rate of infection (40.7%), while children aged 8-10

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Publication Date
Mon May 28 2018
Journal Name
Iraqi Journal Of Science
3D Geological Model For Khasib, Tanuma, and Sa'di formations of Halfaya Oil Field in Missan Governorate-Southern Iraq

     A geological model is a spatial representation of the distribution of sediments and rocks in the subsurface. Where this study on Halfaya oil field; it is located in Missan governorate, 35 km southeast of the city of Amara. It is one of the main  fields in Iraq because it is production high oil. This model contains the structure, and petrophysical properties (porosity,  water saturation) in three directions. To build 3D geological models of petroleum reservoirs. Khasib, Tanuma, and Sa’di formations in Halfaya oil field have been divided into many layers depending on petrophysical properties and facies. 

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Publication Date
Fri Feb 01 2019
Journal Name
Journal Of Plastic, Reconstructive & Aesthetic Surgery
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Publication Date
Tue Dec 01 2020
Journal Name
The Saudi Dental Journal
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Publication Date
Fri Jun 15 2018
Journal Name
Journal Of Baghdad College Of Dentistry
Assessment of Dental Implant Site Dimensions And Alveolar Bone Density in The Mandible Using Cone Beam Computed Tomography

Background: The quantity and the quality of available bone, influence the clinical success of dental implants surgery. Cone beam Computed tomography is an established method for acquiring bone images before performing dental implant. Cone beam computed tomography is an essential tool for treatment planning and post-surgical procedure monitoring, by providing highly accurate 3-D images of the patient's anatomy from a single, low-radiation scan which yields high resolution images with favorable accuracy. The aim of study is the Measurement of alveolar bone (height and buccolingual width) and density in the mandible among Iraqi adult subject using CBCT for assessment of dental implant site dimensions. Material and method: The study sample in

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Publication Date
Mon Jul 01 2013
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Homestasis Model Assessment–Adiponectin ratio and Adiponectin –Resistin index as markers of insulin resistance in type 2diabetes mellitus

Background: Insulin resistance (IR) is an important clinical and biochemical determinant of diabetes and many other clinical states. The gold standard method for IR estimation is the hyperinsulinemic euglycaemic glucose clamp. Adiponectin and resistin are adipocytes derived hormones that play important role in the regulation of glucose, fat metabolism and insulin resistance.
Objective: this study aimed to assess the validity of Homestasis Model Assessment–Adiponectin ratio and Adiponectin –Resistin index as markers of insulin resistance in type 2 diabetic mellitus (T2DM) patients.
Design: Ninety (T2DM) overweight male subjects were enrolled in this cross sectional study, detailed history, physical examination and anthropometric

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Publication Date
Thu Sep 30 2021
Journal Name
Iraqi Journal Of Science
Evaluating the in vitro Cytotoxicity of Thymus vulgaris Essential Oil on MCF-7 and HeLa Cancer Cell Lines

     Thyme essential oil (TEO) was extracted from dried leaves of Thymus vulgaris. The air-dried aerial parts of the plant produced 1.0% yield of TEO. The detection of this essential oil’s compounds was performed by GC-MASS. The cytotoxic activity of TEO was evaluated against two human cancer cell lines, namely HeLa (human epithelial cervical cancer) and MCF-7 (human breast carcinoma). Cells grown in 96 multi-well plates were treated with six concentrations of EO (6.25, 12.5, 25, 50, 100, 200 ppm) and incubated at 37 °C for 72 hrs. Cancer cell lines elicited various degrees of sensitivity to the cytotoxic effect of essential oil. The TEO exhibited significant differences (p≤ 0.01) between the effects of

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Publication Date
Tue Jun 30 2015
Journal Name
Al-kindy College Medical Journal
Estimation of vitamin E level and its relation to lipid profile in patients with type II Diabetes Mellitus

Background: Type 2 diabetes mellitus (T2DM) is considered a global disease as it affects over 150 million people worldwide, a number that is supposed to be doubled by 2025. High glucose levels, in vitro, appear to raise the extent of LDL oxidation, and glycated LDL is more prone to oxidative modification.Objective: To investigate the relationship between serum level of vitamin E and lipid profile in patients with type II DM.Methods: This study involved 28 patients suffering from type II DM diagnosed 1-4 years ago and with age ranged from 17 -60 years old, with different residence around Basra ; In addition to 56 apparently healthy persons matched in age and sex to the patients as a control group. The medical histories were taken and Gene

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