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Bacteria strain H7, which produces flocculating substances, was isolated from the soil of
corn field at the College of Agriculture in Abu-Ghrib/Iraq, and identified as Bacillus subtilis
by its biochemical /physiological characteristics. The biochemical analysis of the partially
purified bioflocculant revealed that it was a proteoglycan composed of 93.2 % carbohydrate
and 6.1 % protein. The effects of bioflocculant dosage, temperature, pH, and different salts on
the flocculation activity were evaluated. The maximum flocculation activity was observed at
an optimum bioflocculant dosage of 0.2 mL /10 mL (49.6%). The bioflocculant had strong
thermal stability within the range of 30-80 °C, and the flocculating activity was over 50 %.
The biofloc
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