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bijps-1643
Occurrence of Quinolone-Resistance Genes in Ciprofloxacin-Resistant Salmonella Enterica Serotype Typhi Isolated form Blood Sample of Patients with Typhoid Fever
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Salmonella is approved as a common foodborne pathogen, causing major health problems throughout the world particularly in low‐ and middle‐income countries. Low-level fluoroquinolone resistance is conferred by both chromosomal and plasmid-encoded resistance, this research was carried out look into the occurrence rate of qnrA,qnrB and qnrS genes  in  Salmonella enterica serotype Typhi Cipr  ofloxacin-resistant insulate from blood samples of patients with typhoid fever. Fifteen Salmonella enterica serotype Typhi isolated previously from patients with typhoid fever were included in this study. All bacterial isolates were confirmed to have ciprofloxacin resistant by VITEK 2 microbial identification system; after plasmid DNA extraction; multiplex-PCR was done with primer sequences intended to plasmid-mediated quinolone-resistance genes which is qnrA, qnrB, and qnrS. In this study; it was 21 qur genes amongst 15 isolates. The qnrS gene was the commonest (10/21, 47.6%) followed by qnrA (6/21, 28.5%), whereas only 4 isolates were positive for qnrB (5/21, 23.8%). Some isolates had more than one qnr genes. So, Ciprofloxacin-resistant Salmonella typhi can have more than one gene at the same time; and the most occurrence rate in regards to qnr gene in this study was qnrS compared to qnrA and qnrB

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Publication Date
Thu Mar 30 2023
Journal Name
Iraqi Journal Of Science
Effect of aqueous red cabbage extract on uricase activity isolated from Pseudomonas aeruginosa
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Separation of uricase from Pseudomonas aeruginosa was done using (70%) satu-ration ammonium sulphate, and purification of this enzyme was done by ion ex-change chromatography on DEAE- cellulose column and eluted with linear NaCl (0-1M). Partial purified uricase gave an activity of (4.9 u/ml), protein concentration of (0.56 mg/ml), specific activity of (8.75 unit/mg) with purification folds (8.4) and a yield of (48%). The maximum purified uricase activity was detected at 35ºc and pH 8.5 with (0.12 mM.uric acid). The results shown that red cabbage extract (RCE) contain flavonoides which contain phenolic compounds and anthocyanines which glycoslated with mono or dimolecules of saccharides, while test for alkaloids, ster-oids, saponins and

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Publication Date
Wed Jul 06 2022
Journal Name
International Journal Of Biomaterials
Extracellular Enzyme of Endophytic Fungi Isolated from Ziziphus spina Leaves as Medicinal Plant
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Endophytic fungi live inside plants or any part of them without creating any visible pathogenic signs. Endophytic fungi are found within medicinal plants and have shown strong biologic activity, such as anticancer and antioxidant activities, as well as producing extracellular enzymes. In this study, different fungal strains were isolated from the leaves of the medicinal plant Ziziphus spina, including Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Cladosporium sp., Rhizopus sp., and Mucor sp. Extracellular enzymes have been quantified using agar plate-based methods in which fungi were grown in specified growth media to detect the enzymes produced. The results showed that A. niger has the highest ability to produce amy

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Publication Date
Wed Jul 29 2020
Journal Name
Iraqi Journal Of Science
Molecular Comparison of Free -Living Amoeba Isolated From Iraqi, Iranian and Turkish Waters
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Amoebas live freely in different climates and parts of the world. Several species of Free Living Amoeba (FLA) are capable of causing serious as well as fatal infections in human beings. The aim of this study was to identify and compare genotypes of water-polluting FLA in major rivers and lakes of Iraq and compare them with FLA isolates from Iran and Turkey. For this purpose, the study included 20 water samples from the Tigris River, Euphrates River , Najaf Sea and Dukan lake in Iraq, 20 water samples from  Marivan, Velasht, and Soleimanshah lakes and Caspian sea in Iran, and 20 water samples from Sabanca, Seyfi , Hazar and Yay lakes in Turkey. The samples were studied by culture methods, invert microscope, and molecular methods.

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Publication Date
Thu Sep 30 2021
Journal Name
Iraqi Journal Of Science
Establishment of Lipid Profile Reference Intervals in a Sample Population of Halabja City, Kurdistan Region of Iraq
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     Every laboratory needs to establish its own community-based reference intervals for the achievement of the optimal and true diagnosis in the clinical practice. However, such vital experimentations are not been conducted sufficiently in Halabja city and Kurdistan region of Iraq as a whole. This study is an attempt to introduce for the first time a set of reference intervals of lipid profile variables that are based on the healthy and asymptomatic adult population of Halabja using international standards. The methodology recruited for this purpose is based on the IFCC, CLSI, and WHO’s standards, in which the study population was subjected to strict exclusion criteria for the sake of higher accuracy in the results

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Publication Date
Mon Nov 01 2021
Journal Name
Journal Of Physics: Conference Series
Light scattering detector based on light-emitting diodes-Solar cells for a flow analysis of Warfarin in pure form and pharmaceutical formulations
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Abstract<p>Continuous turbidimetric analysis (CTA) for a distinctive analytical application by employing a homemade analyser (NAG Dual & Solo 0-180°) which contained two consecutive detection zones (measuring cells 1 & 2) is described. The analyser works based on light-emitting diodes as a light source and a set of solar cells as a light detector for turbidity measurements without needing further fibres or lenses. Formation of a turbid precipitated product with yellow colour due to the reaction between the warfarin and the precipitation reagent (Potassium dichromate) is what the developed method is based on. The CTA method was applied to determine the warfarin in pure form and pharmaceu</p> ... Show More
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Publication Date
Tue Mar 26 2019
Journal Name
Iraqi Journal Of Science
Study the Effect Some of Electrodeposition Parameters on Wear Resistance for Cu-MWCNT Composite Coating
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A Copper- Mullet walled Carbon Nanotube Cu/MWCNT composite coatings prepared by direct and pulse electrodeposition with various contents of MWCNT particles, using electrodeposition method from acidic sulfate bath. The effect of the current density, pulse frequency and  particle loading (PL) of MWCNT particle in the electrolyte on the morphology and volume fraction Vol% of MWCNT in deposit were investigate. The experimental results indicated that the depositing condition affect on the microstructure of deposited copper and MWCNT Vol% in deposit. Also, the Vol% of MWCNT increases in deposit with particle loading (PL) increasing in the bath and increased with increased current density. Increasing MWCNTs in electrolyte produced increase

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Publication Date
Sun Dec 01 2019
Journal Name
Journal Of Accounting And Financial Studies ( Jafs )
Use of information and communications technology to archive data: A suggested form in the Tax Audit and Examination Department of the General Tax Authority
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The current world is observing huge developments in presenting the opportunity for organizations and administrative units to use information and communication technology and their adoption by administrative work due to its importance in the achievement of work with higher efficiency, speed, and facility of communication with all individuals and companies using various means of communication Depending on the Internet networks. Therefore, the research dealt with the study of electronic systems designed and adopted in the creation or construction of a database for archiving data, which is the main method in organizations and administrative units in developed countries. Where this system works to convert documents, and manual processes and t

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Publication Date
Tue Aug 01 2023
Journal Name
Baghdad Science Journal
Bioethanol Production by Candida tropicalis Isolated from Sheep Dung
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 Microorganisms have an active role in biotechnology for example yeasts, especially in some genus like Saccharomyces, Pichia, and CandidaC.tropicalis one of the most important species of Candida and despite it is one of the causative agents of candidiasis but it has a major role in the production of many chemical compounds. C.tropicalis  in the previous study was isolated from sheep dung and morphologically and molecularly classified the result of sequencing was elucidate 100% similarity between the studied isolate and other isolates inserted in DNA Data Bank of Japan DDBJ, physiologically this isolate tolerated 6% ethanol concentration in broth media with the ability to the pro

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Publication Date
Sun Mar 13 2011
Journal Name
Baghdad Science Journal
Lead bioremoval by Bacillus spp. Isolated from industrial wastewater
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Four samples were collected from the wastewater of State Battery Manufacturing Company (SBMC); Babylon 2 factory in AL-Waziriya district, as triplicates. Physical and chemical measurements were carried out such as temperature, pH, Lead concentrations and their ranges were: (19.5-34.5) °C, (6.1-6.4) and (4.5-6.5) mg/L, respectively. Six dominant Bacillus spp. isolates were isolated from these samples; namely, Bacillus subtilis N1, Bacillus subtilis N2, Bacillus subtilis N3, Bacillus cereus N4, Bacillus cereus N5 , Bacillus cereus N6. These isolates were capable of removing Lead from aqueous solutions in a capacity reached 27.6 ± 1.4, 10.1 ± 1.7, 74.5 ± 0.7, 8.93 ± 2.8, 8.1 ± 3.5, 1.6± 0.7 mg/L, respectively. Whereas cell walls,

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Publication Date
Sun Jun 03 2012
Journal Name
Baghdad Science Journal
Studying Proteus mirabilis Typing Isolated from Urinary Tract infections
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Urine specimen (253 samples) had been collected from urinary tract infections. The study showed that Proteus mirabilis was responsible for (11.85%) of the urinary tract infections. Also, the study had declared that the ratio of isolation of this bacterium from women was (7.51%) and it was higher than the ratio in both men and children which ranged (1.58%) and (2.76%) respectively. Morphological and biochemical studies had been applied to characterize this bacterium as well as other kinds of microorganisms that were isolated from urinary tract infection in this study. The study deals with typing methods such as using biotyping and typing according to Dienes phenomenon beside the succestibility to antibiotics. The results had shown that the s

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