Extraction of Penicillin V from Simulated Fermentation  Broth by Liquid-Liquid Membrane Technique

Khalid W. Hameed

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Publication Date

Thu May 31 2012

Journal Name

Al-khwarizmi Engineering Journal

Volume

8

Issue Number

2

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Extraction of Penicillin V from Simulated Fermentation Broth by Liquid-Liquid Membrane Technique

Liquid-liquid membrane

Penicillin V

pertraction

extraction

Khalid W. Hameed

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Liquid-liquid membrane extraction technique, pertraction, using three types of solvents (methyl isobutyl ketone, n-butyl acetate, and n-amyl acetate) was used for recovery of penicillin V from simulated fermentation broth under various operating conditions of pH value (4-6) for feed and (6-8) for receiver phase, time (0-40 min), and agitation speed (300-500 rpm) in a batch laboratory unit system. The optimum conditions for extraction were at pH of 4 for feed, and 8 for receiver phase, rotation speed of 500 rpm, time of 40 min, and solvent of MIBK as membrane, where more than 98% of penicillin was extracted.

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Publication Date

Mon Apr 06 2020

Journal Name

August

INFLUENCE OF SOME FACTOR ON SOMATIC EMBRYOS INDUCTION AND GERMINATION OF DATE PALM CV BARHI BY USING CELL SUSPENSION CULTURE TECHNIQUE

This study was done to examine different concentrations of mercuric chloride (Hgcl2) for Explants sterilization and different concentration of NAA) mg/L with 2iP for their effect on initiation primary callus and subsequent embryogenesis in Phoenix dactyliferae cultivar Barhi. Leaf primordial and shoot tips were excised from 2-3 years old offshoot. The result showed that contamination percentage decreased with an increase in concentration of Hgcl2 for two explants where in the control (0.0)% gave highest value 75% and 62.5% of contamination for leaf primordial and shoot tip respectively while the addition (0.1)% gave a significant decrease 37.5 and 0% for explants respectively. The Explants were inoculated onto Murashiege and Skoog

1962 (MS) medium supplemented with 2iP 3mg/L and containing 0

25

50 or 100 mg/L of NAA

the addition (50) mg/L of NAA gave highest value 68.8% in percentage of callus initiation with significant difference from addition 0% gave lowest value (18.8)%

as well Explants of shoot tip gave significant difference was 53.1% while leaf primordial gave 28.1%

Primary callus was transferred to fresh MS medium containing (0

5

10 and 20) mg/L of NAA with 3mg/L 2iP

Results were recorded after 12 weeks. A highest value of embryonic callus induction percentage or fresh weight and dry weight 87.5% or 1.56g and 0.47g for the character respectively for the treat 20 mg/L while control treatment 0.0 gave the lowest value 12.5% or 0.37g and 0.14g for same character respectively. Inoculated 1g fresh biomass into a 250 ml Erlenmeyer flask containing 50 ml MS liquid medium supplemented with NAA 20mg/L and agitated at 150 rpm by shaker to making suspension culture the measurements of packed cell volume ( pcv) or fresh and dry weight taken weekly for 12 weeks

maximum values were reached in the eighth week of PCV and fresh weight but the dry weight reached to highest value in seventh week

then the curve began to decline gradually until it reached stability in the last two weeks. The cell suspension was Diluted to different initial cell density of 100

500

10000

50000 and 100000 cells /ml with hemocytometer. The initial cell number of (10000) cells gave significant different of plating efficiency PE (11.6)% while (100) cells gave lowest value 1.8% of PE.

Ali A.

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Publication Date

Thu Mar 18 2021

Journal Name

Egyptian Journal Of Chemistry

Investigation of the Influence of Membrane Type on the Performance of Microbial Fuel Cell

Hayder

Ahmed

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(3)

(1)

Publication Date

Sun Nov 01 2020

Journal Name

Iraqi Journal Of Laser

Rejuvenation of Facial Skin Using Fractional Er: YAG Laser

Facial rejuvenation

Fractional Er: YAG

Multi stack

Variable pulse width.

Ali A.

Athir M.

Ali S.

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Fractional Er: YAG laser resurfacing is increasingly used for treating rhytides and photo aged skin because of its favorable benefit‐risk ratio. The multi-stacking and variable pulse width technology opened a wide horizon of rejuvenation treatments using this type of laser. To evaluate the efficacy and safety of the use of fractional 2940 nm Er: YAG laser in facial skin rejuvenation. Twelve female patients with mean age 48.3 years and multiple degrees of aging signs and solar skin damages, were treated with 2 sessions, one month apart by fractional Er: YAG laser. Each session consisted of 2 steps, the first step employed the use of the multi stack ablative fractional mode and the fractional long pulsed non-ablative mode settings were u

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Publication Date

Tue Jun 01 2021

Journal Name

Minar International Journal Of Applied Sciences And Technology

STRUCTURAL AND ELECTRICAL PROPERTIES OF (CDO)1-X (V2O5)X PREPARED BY PULSE LASER DEPOSITION TECHNIQUE FOR SOLAR CELL APPLICATIONS

Asmaa Natiq Mohammed

Lamia K

Ihab Abbas

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In this work ,pure and doped(CdO)thin films with different concentration of V2O5x (0.0, 0.05, 0.1 ) wt.% have been prepared on glass substrate at room temperature using Pulse Laser Deposition technique(PLD).The focused Nd:YAG laser beam at 800 mJ with a frequency second radiation at 1064 nm (pulse width 9 ns) repetition frequency (6 Hz), for 500 laser pulses incident on the target surface At first ,The pellets of (CdO)1-x(V2O5)x at different V2O5 contents were sintered to a temperature of 773K for one hours.Then films of (CdO)1-x(V2O5)x have been prepared.The structure of the thin films was examined by using (XRD) analysis..Hall effect has been measured in orded to know the type of conductivity, Finally the solar cell and the effici

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Publication Date

Fri Sep 01 2023

Journal Name

Journal Of Optics

Structural and optical properties of C60-ZnO thin films synthesized by spray pyrolysis technique with plasma treatment as antibacterial activity

Ahmed A.F.

Falah A. -H. Mutlak

Mohammed R. Abdulameer

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Spray pyrolysis technique was used to make Carbon60-Zinc oxide (C60-ZnO) thin films, and chemical, structural, antibacterial, and optical characterizations regarding such nanocomposite have been done prior to and following treatment. Fullerene peaks in C60-ZnO thin films are identical and appear at the same angles. Following the treatment of the plasma, the existence regarding fullerene peaks in the thin films investigated suggests that the crystallographic quality related to C60-ZnO thin films has enhanced. Following plasma treatment, field emission scanning electron microscopy (FESEM) images regarding a C60-ZnO thin film indicate that both zinc oxide and fullerene particles had shrunk in the size and have an even distribution. In addition

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(1)

Publication Date

Wed Mar 25 2020

Journal Name

International Journal Of Drug Delivery Technology

Detection of Single Nucleotide Polymorphisms (SNPs) for Genes Cause Drug-Resistant in Iraqi Mycobacterium Tuberculosis isolates by new Pyrophosphate Technique.

Hassan Kadhim

Ismail

Alaa K.

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In this search, a new pyrophosphate technique was proved. The technique was employed to single- nucleotide polymorphisms (SNPs), which diagnosis using a one-base extension reaction. Three Mycobacterium tuberculosis genes were chosen (Rpob, InhA, KatG) genes. Fifty-four specimens were used in this study fifty-three proved as drug-resistant specimens by The Iraqi Institute of Chest and Respiratory Diseases in Baghdad.; also one specimen was used as a negative control. The steps of this technique were by used a specific primer within each aliquot that has a short 3-OH end of the base of the target gene that was hybridized to the single-stranded DNA template. Then, the Taq polymerase enzyme and one of either α-thio-dATP, dTTP, dGTP, or dCTP

Publication Date

Fri Sep 09 2022

Journal Name

Journal Of Ovonic Research

The effects of CuO doping on structural, electrical and optical properties of CdO thin films deposited by pulsed laser deposition technique

S. M. A.

Nathera

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Thin films of (CdO)x (CuO)1-x (where x = 0.0, 0.2, 0.3, 0.4 and 0.5) were prepared by the pulsed laser deposition. The CuO addition caused an increase in diffraction peaks intensity at (111) and a decrease in diffraction peaks intensity at (200). As CuO content increases, the band gap increases to a maximum of 3.51 eV, maximum resistivity of 8.251x 104 Ω.cm with mobility of 199.5 cm2 / V.s, when x= 0.5. The results show that the conductivity is ntype when x value was changed in the range (0 to 0.4) but further addition of CuO converted the samples to p-type.

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(9)

(10)

Publication Date

Mon Oct 05 2026

Journal Name

Iraqi Journal Of Applied Physics

Effect of Gas Mixing Ratio on Energy Band Gap of Mixed-Phase Titanium Dioxide Nanostructures Prepared by Reactive Magnetron Sputtering Technique

E Adnan

Firas

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Publication Date

Thu Oct 01 2020

Journal Name

August

INFLUENCE OF SOME FACTOR ON SOMATIC EMBRYOS INDUCTION AND GERMINATION OF DATE PALM BARHI C.V BY USING CELL SUSPENSION CULTURE TECHNIQUE

Ali . A

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INFLUENCE OF SOME FACTOR ON SOMATIC EMBRYOS INDUCTION AND GERMINATION OF DATE PALM BARHI C.V BY USING CELL SUSPENSION CULTURE TECHNIQUE

Publication Date

Fri Mar 20 2020

Journal Name

Plant Archives (09725210) 20 (2)‏

Influence of some factor on somatic embryos induction and germination of date palm CV barhi by using cell suspension culture technique.‏

Moayad Rajab Abood

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