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Characterization of the major human STAG3 variants using some proteomics and bioinformatics assays
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STAG3 is the meiotic component of cohesin and a member of the Cancer Testis Antigen (CTA) family. This gene has been found to be overexpressed in many types of cancer, and recently, its variants have been implicated in other disorders and many human diseases. Therefore, this study aimed to analyze the major variants of STAG3. Western blot (WB) and immunoprecipitation (IP) assays were performed using two different anti-STAG3 antibodies that targeted the relevant protein in MCF-7, T-47D, MDA-MB-468, and MDA-MB-231 breast cancer cells with Jurkat and MCF-10A cells as positive and negative controls, respectively. In silico analyses were searched to study the major isoforms. WB and IP assays revealed two abundant polypeptides < 191 kDa and ~ 75 kDa in size. Specific bioinformatics tools successfully determined the three-dimensional (3-D) structure, the subcellular localization, and the secondary structures of the isoforms. Furthermore, some of the physicochemical properties of the STAG3 proteins were also determined. The results of this study revealed the power of applying the biological techniques (WB and IP) with the bioinformatics assays and the possibility of their exploitation in understanding diseased genes. Exploring the major variants of STAG3 at the protein level could help decipher some disorders associated with their occurrence, along with designing drugs effective at least for some relevant diseases.

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Publication Date
Thu Jan 01 2015
Journal Name
International Journal Of Science And Research
Synthesis and Spectroscopic Characterization of some Mixed Schiff Base Complexes
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Publication Date
Sun Jan 01 2023
Journal Name
Materials Today: Proceedings
Synthesis and characterization of some mixed ligands complexes of β-enaminone with some metal ions
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Publication Date
Thu Jul 01 2021
Journal Name
Journal Of Physics: Conference Series
Human Dynamic Behavior: Reconstruction Trajectories Using CDRs
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Abstract<p>Investigating the human mobility patterns is a highly interesting field in the 21th century, and it takes vast attention from multi-disciplinary scientists in physics, economic, social, computer, engineering…etc. depending on the concept that relates between human mobility patterns and their communications. Hence, the necessity for a rich repository of data has emerged. Therefore, the most powerful solution is the usage of GSM network data, which gives millions of Call Details Records gained from urban regions. However, the available data still have shortcomings, because it gives only the indication of spatio-temporal data at only the moment of mobile communication activities. In th</p> ... Show More
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Publication Date
Sun Sep 24 2023
Journal Name
Journal Of Al-qadisiyah For Computer Science And Mathematics
Human Recognition Using Ear Features: A Review
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Over the past few years, ear biometrics has attracted a lot of attention. It is a trusted biometric for the identification and recognition of humans due to its consistent shape and rich texture variation. The ear presents an attractive solution since it is visible, ear images are easily captured, and the ear structure remains relatively stable over time.  In this paper, a comprehensive review of prior research was conducted to establish the efficacy of utilizing ear features for individual identification through the employment of both manually-crafted features and deep-learning approaches. The objective of this model is to present the accuracy rate of person identification systems based on either manually-crafted features such as D

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Publication Date
Sun Aug 07 2022
Journal Name
Hiv Nursing
Assessment of ApoE Gene Variants and Apob-100 R3500q Mutation as Genetic Risks for Dyslipidemia: A Case-Control Study
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Background: Dyslipidemia is defined as an abnormally high level of various lipids in the blood. It is considered a major risk for atherosclerosis and coronary artery disease. Genetic susceptibility can have a significant influence on the development and progression of dyslipidemia. ApoB-100 R3500Q mutation and ApoE variants are among those genetic risks for dyslipidemia. This study aims to assess the possible contribution of ApoB and ApoE variants on lipid profile among a group of early-onset ischemic heart disease (IHD) patients in comparison to a group of controls. Methods: Forty patients with dyslipidemia and early-onset IHD without chronic conditions likely to cause derangement of lipid levels were recruited to this case-control study

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Publication Date
Sun Dec 03 2017
Journal Name
Baghdad Science Journal
The Effect of Anesthesia on Thyroid Hormones and Cortisol Levels Following Major Urological Surgery
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This study was designed to evaluate the effect of anesthesia on thyroid hormones (T3, T4, rT3), thyrotropin(TSH), and cortisol levels in patients undergoing major urological surgery. The study included fifty patients scheduled for elective major urological surgery where 70% of the patients underwent general anesthesia, while30% underwent regional anesthesia. All patients were in stable control general conditions. The study showed that the thyroid hormones, thyrotropin and cortisol levels were affected with general anesthesia more than regional. The levels of these hormones responded to anesthetics and surgical stresses. This response was thought to be mainly mediated by afferent neurogenic impulses from the area of injury. Therefore, ab

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Publication Date
Sun Jul 06 2014
Journal Name
Journal Of Educational And Psychological Researches
The Intellectual Stress and Its Relationship with Personality' s Five Major Factors of Counselors
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The current study aims at identifying:

1-The intellectual stress of counselors according to gender variable (male, female ).

2-The five major  factors of the  Counselor's  Personality.

3-  The relationship between intellectual stress and the five major factors of the councilors' personality and according to gender variable (male, female ).

4-The significant differences in the relationship between intellectual stress and the five major factors of the councilors' personality and according to gender variable  (male, female ).

The sample of the study includes (260) counselors (130 male

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Publication Date
Sat Mar 01 2008
Journal Name
Iraqi Journal Of Physics
Effect of Nd-YAG, XeCl, and Nitrogen Laser Radiation on Human Aorta , and Some Arterial Tissues
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The effect of laser radiation on human aorta, coronary, and pulmonary arteries, and pulmonary veins has been investigated. Xenon-Chloride (eximer), Nitrogen, and Nd-YAG pulsed lasers of wavelengths 308, 337, and 1060 nm respectively were used. Their effects on fresh postmortem tissues, normal and diseased, was studied. The diameter and depth of ablation of the exposed tissues, in air, were measured as a function of many factors related to the type of laser and nature of the tissue. The effect of properties of the applied lasers, such as average power density and deposited energy density, on the exposed tissue surface were studied. The increase of these two parameters cause an increase in the depth and diameter of ablation. However the di

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Publication Date
Sun Sep 05 2010
Journal Name
Baghdad Science Journal
Effect of some species of bacteria on viability of human hydatid cysts
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A total of 50 fertile human hydatid cases {33(66%) females and (34%) males}, obtained from Al-Ramadi public Hospital during the period from December 2003 to July 2004 were examined to study any bacterial infections. The specimens were obtained from hydatid fluid and then cultured on appropriate culture media to distinguish some species of bacteria which resulted in obtaining: Staphylococcus aureus (18%), Pseudomonas aeruginosa(12%), Escherichia coli(6%) and Streptococcus pneumonia (4%). These bacteria were confirmed by isolation from interacyst fluid and blood culture technique. The possible routs of infection may be through blood, biliary ducts and bronchioles .The selectivity permeable of the cyst wall may be absent and that may allow

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Publication Date
Fri Feb 05 2021
Journal Name
Proceeding Of The 1st Conference On Dust Storms And Their Environmental Effects
Deposition of Major and Trace Elements From Atmosphere Over Baghdad During the Year of 2010.
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