Several streptococcal species traverse the human-animal-environment interface, positioning them as particularly compelling subjects for One Health research. In contrast to strictly host-adapted pathogens, these species are observed to circulate among livestock, companion animals, aquaculture, and the broader environment, exhibiting demonstrable transmission to human populations. In this study, 200 swabs were collected from humans and animals (100 nasal and oral swabs were tested bacteriologically from individuals with respiratory infections and gastrointestinal illnesses of all genders and ages. 100 swabs were collected from pets (dogs and cats) of both genders and various ages. Various respiratory symptoms were tested to isolate and characterize the bacterial causes of respiratory infections and to identify bacteria that can infect both humans and their pets. Finally, the results were confirmed by primary culture media, the Vitk 2 system, and 16 S rRNA. The results of bacterial isolation from the human nose showed the presence of six streptococcal isolates, classified into three types: S. thoraltensis (3, 50%), followed by S. anginosus (2, 33.33%), and finally S. alactolyticus (1, 16.66%). Moreover, the results from the human mouth showed the existence of 15 streptococcal isolates, divided into four types: S. salivarius (5, 33.33%), followed by S. thoraltensis (4, 26.66%), then S. alactolyticus and S. parasanguinis (3, 20%). At the same time, the results from the dog nose showed the presence of four streptococcal isolates, classified into three types: S. anginosus (2, 50%), followed by S. thoraltensis and S. alactolyticus (1, 25%). The results from the dog oral samples showed the presence of 16 streptococcal isolates, divided into four types: S. salivarius (6, 37.5%), S. alactolyticus (4, 25%), and S. thoraltensis and S. parasanguinis (3, 18.75%). Moreover, the cat nose results showed the presence of three streptococcal isolates, all classified as S. anginosus (100%). The results from the cat oral swab showed the presence of 8 streptococcal isolates, divided into four types: S. thoraltensis (3; 37.5%), S. parasanguinis and S. alactolyticus (2; 25%), and S. salivarius (1; 12.5%). Additionally, the identification of 6 Streptococcus sp. strains was confirmed using the 16 S rRNA gene.
The current study was conducted to determine the sensitivity of some pathogenic bacterial isolates isolated from wounds and burns water toward the disposer of the Yas Rue tested five crude bacterial isolates isolated from wounds and burns which these isolates sensitive to aqueous extract crude
Cryptosporidiosis is an intestinal protozoan parasitic disease that infects human and animals, caused by apicomplexan parasite belong to the genusof Cryptosporidium. The current study was done to record the infection rate of cryptosporidiosis in human and cattle, and genotype the clinical isolates of Cryptosporidium in Baghdad Province. A total of 265 stool sample were collected (150 from human and 115 from cattle) during the period from December 2016 to the May 2017. Cryptosporidial infection was detected using modified acid fast stain. DNA of the parasite was extracted from oocysts of positive fecal samples and nested PCR method was used for partial 60 kDa glycoprotein (gp60) gene amplification then sequence analysis for selected samples.
... Show MoreObjective: Synthesis, Characterization of formazan derivatives and studies the antioxidant activity of prepared compounds and molecular docking. Methods: In this study, formazan compounds (III–XIV) were produced by combining Schiff base compounds (I), (II) with diazonium salts resulting from reactions of different aromatic amines with sodium nitrate in the presence of Con.HCl at 0–5°C. When isonicotinic acid hydrazide reacts with (N,N-dimethylbenzaldehyde, 4-hydroxy-3-methoxybenzaldehyde) in the presence glacial acetic acid as a solvent Schiff base compounds are created. Results: The prepared compounds were identified by FT-IR, 1H NMR, 13C NMR, then the antioxidant activity of the derivatives and molecular docking were studied. D
... Show MoreLeuciscidae species are the abundant and widely distributed fish species in Iraq's inland waters. They are complex species, and morphology makes them difficult to identify. Molecular analysis achieved and confirmed the morphological characters. Twenty specimens of Acanthobrama marmid were collected from two localities at Tigris River, in the middle of Iraq; 15 specimens from the Al-Zubaydia sub-district and five specimens from Al-Tharthar Lake. We used the mitochondrial DNA cytochrome b (cytb) gene to sequence the DNA of A. marmid. The following analysis are compared the sequences with those of other fish genera and species found in the Gene Bank. The barcoding result (DNA sequencing) in fishes found in the same family (Leuciscidae) showed
... Show MoreLeuciscidae species are the abundant and widely distributed fish species in Iraq's inland waters. They are complex species, and morphology makes them difficult to identify. Molecular analysis achieved and confirmed the morphological characters. Twenty specimens of Acanthobrama marmid were collected from two localities at Tigris River, in the middle of Iraq; 15 specimens from the Al-Zubaydia sub-district and five specimens from Al-Tharthar Lake. We used the mitochondrial DNA cytochrome b (cytb) gene to sequence the DNA of A. marmid. The following analysis are compared the sequences with those of other fish genera and species found in the Gene Bank. The barcoding result (DNA sequencing) in fishes found in the same family (L
... Show MoreBackground: The frontal sinus area can be used as a diagnostic aid to recognize mouth breather subjects. The aims of this study were to determine the gender difference in each group, to compare the frontal sinus area between mouth breather and nasal breather group, and to verify the presence of correlation between the frontal sinus area and the cephalometric skeletal measurements used in this study. Materials and Methods: Cephalometric radiographs were taken for 60 adults (30 mouth breathers and 30 nasal breathers) age range (18-25), for each group 15 males and 15 females, in the orthodontic clinic in the college of Dentistry at Baghdad University. The control group (nasal breather) with skeletal class I and ANB angle ranged between 2-4º,
... Show MoreMicroalgae have been increasingly used for wastewater treatment due to their capacity to assimilate nutrients. Samples of wastewater were taken from the Erbil wastewater channel near Dhahibha village in northern Iraq. The microalga Coelastrella sp. was used in three doses (0.2, 1, and 2g. l-1) in this experiment for 21 days, samples were periodically (every 3 days) analyzed for physicochemical parameters such as pH, EC, Phosphate, Nitrate, and BOD5, in addition to, Chlorophyll a concentration. Results showed that the highest dose 2g.l-1 was the most effective dose for removing nutrients, confirmed by significant differences (p≤0.05) between all doses. The highest removal percentage was
... Show MoreTwo modes of electrochemical harvesting for microalgae were investigated in the current work. A sacrificial anode (aluminum) was used to study the electrocoagulation-flotation process, and a nonsacrificial anode (graphite) was used to investigate the electroflotation process. The study inspected the effect of chloride ions concentration and the interelectrode distance on the performance of the electrochemical harvesting processes. The results demonstrated that both electrodes achieved maximum harvesting efficiency with a 2 g/L NaCl concentration. Interestingly, by increasing the NaCl concentration to 5 g/L, the harvesting efficiency reduced dramatically to its lowest value. Generally, the energy consumption decreased with increasing
... Show MoreAim of this study was to identify and characterize the entomopathogenic nematode Metarhabditis rainai, which was isolated from soil samples carried out from Alrashdya at (33˚25ˈ13.4̎ N 44˚21ˈ45.3̎ E) Baghdad- Iraq and was illustrated by both morphological and molecular aspects. All specimens of M. rainai were cultured, identified and described using some morphometric criteria based on standard available key. Selected specimens (Zah, IQ2 OR960470 isolate) of this species were characterized by the body length of male was ranged from (377.49 – 563.89 μm), the body length of female was (857.44 – 1111.7 μm) and the body length of juvenile was (419.28 – 757 μm). Using partial 18S rRNA gene sequences, molecular characteriz
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