Phylogenetic Relationship of Iraqi Vespa orientalis Linnaeus 1771 Wasps Using Mitochondrial CO1

Afrah Abdulzahra Aljassani; Mushtaq Al-Esawy; Razzaq Shalan Augul; Osamah Alisawi

doi:10.1088/1755-1315/1262/3/032016

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Publication Date

Fri Dec 01 2023

Journal Name

Iop Conference Series: Earth And Environmental Science

Volume

1262

DOI

10.1088/1755-1315/1262/3/032016

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Phylogenetic Relationship of Iraqi Vespa orientalis Linnaeus 1771 Wasps Using Mitochondrial CO1

Afrah Abdulzahra Aljassani

Mushtaq Al-Esawy

Razzaq Shalan Augul

Osamah Alisawi

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AbstractOriental wasps are scavengers, and they have also represented an enormous issue for beekeepers, they destroy beehives and reduce the flight of bees. In addition, the sting of hornets may cause medical problems, which differ according to the response of the individuals, including severe sensitivity, swelling, and slight pain. This study provides the first molecular phylogeny of the oriental wasp <italic>Vespa orientalis</italic> L. in Iraq. Mitochondrial DNAs of the 547bp fragment cytochrome oxidase subunit 1 (CO1) area were sequenced and analyzed from 10 wasp specimens collected in the north, middle, and south of Iraq. The CO1 gene found in the Iraqi isolates was used to create a Bayesian interference. Maximum likelihood (ML) was used to create the phylogenetic tree. Results, according to the results of the phylogenetic analysis, the CO1 gene may be used to positively identify every sample, and there is a close connection between the sequences of samples from various regions of Iraq, with 100% of the samples clustering together. PCR successfully amplified 547bp products for all examined oriental wasp samples. Considering the similarity in the sequences, the Iraqi local species are classified as <italic>V. orientalis</italic>. Conclusion, in this study, <italic>V. orientalis</italic> isolates which were collected from different geographical regions in Iraq were closed and there was low variation. The DNA barcode component of CO1 was found to be an effective method for the quick and precise identification of Iraqi oriental wasps.

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Publication Date

Sun Jan 01 2012

Journal Name

Evidence-based Complementary And Alternative Medicine

Gelam Honey Inhibits the Production of Proinflammatory, Mediators NO,<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:msub><mml:mrow><mml:mtext>PGE</mml:mtext></mml:mrow><mml:mtext>2</mml:mtext></mml:msub></mml:mrow></mml:math>, TNF-α, and IL-6 in Carrageenan-Induced Acute Paw Edema in Rats

Saba Zuhair

Kamaruddin

Suzana

Yasmin Anum

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Natural honey is well known for its therapeutic value and has been used in traditional medicine of different cultures throughout the world. The aim of this study was to investigate the anti-inflammatory effect of Malaysian Gelam honey in inflammation-induced rats. Paw edema was induced by a subplantar injection of 1% carrageenan into the rat right hind paw. Rats were treated with the nonsteroidal anti-inflammatory drug (NSAID) Indomethacin (10 mg/kg, p.o.) or Gelam honey at different doses (1 or 2 g/kg, p.o.). The increase in footpad thickness was considered to be edema, which was measured using a dial caliper. Plasma and paw tissue were collected to analyze the production of inflammatory mediators, such as NO, PGE₂

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Publication Date

Tue Jan 18 2022

Journal Name

Materials Science Forum

The Effect of Gamma Radiation on the Manufactured HgBa2Ca2Cu2.4Ag0.6O8+δ Compound

Gamma doses radiation

crystal size

strain

degree of crystallinity

Scherrer

crystallinity and Williamson equations.

zainab j

Balqees A.

Mohammed A.

Kareem A.

Auday H.

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In this article four samples of HgBa₂Ca₂Cu_2.4Ag_0.6O_8+δ were prepared and irradiated with different doses of gamma radiation 6, 8 and 10 Mrad. The effects of gamma irradiation on structure of HgBa₂Ca₂Cu_2.4Ag_0.6O_8+δ samples were characterized using X-ray diffraction. It was concluded that there effect on structure by gamma irradiation. Scherrer, crystallization, and Williamson equations were applied based on the X-ray diffraction diagram and for all gamma doses, to calculate crystal size, strain, and degree of crystallinity. I

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