Polyhydroxyalkanoates (PHAs) have gained much attention as biodegradable polymers, many efforts are being made to minimize the cost of PHAs by finding cheap carbon source depending on the type of microorganism and fermentation conditions. The aims of this study were to evaluate the effects of different glucose concentrations and other important conditions on the PHA production by Bacillus cereus isolated from soil. Polyhydroxyalkanoates PHAs accumulated by soil microorganisms were examined by screening the isolated bacteria using Sudan B Black and Nile Blue staining process. A Gram positive strain was identified using the 16s rRNA gene, deposited in the NCBI GenBank sequence database. Different growth conditions (favorite glucose concentrations 1-8 % (w/v), temperatures and pH) were tested and the growth parameters (sugar consumption, cell counting and Cell Dry Weight CDW) were studied. The extracted polymers were analyzed and characterized using an FTIR spectrophotometer followed by a GC-MS analysis. The pure bacterial strain isolated from soil was deposited in the NCBI GenBank database B. cereus strain ARY73, which showed significant black colored granules (or dark blue) using Sudan B Black stain, it also showed positive to Nile blue A as a high indicator stain for PHA accumulation. B. cereus ARY73 showed high production of PHA using (w/v): 2% glucose and 1% nitrogen source at 35 °C and pH7 yields 79% per Cell Dry Weight and 96 h of incubation. The extracted polymers were analyzed and characterized using an FTIR spectrophotometer confirming the PHA structure. The FTIR spectrophotometer, followed by a GC-MS analysis indicated the Scl-co-mcl PHA structure. This research demonstrates that the isolated strain B. cereus ARY73 was a good candidate for PHA production with a better quality for use in biomedical and other applications. The use of biopolymer in soil, enhanced the accumulation of the microorganisms (such as bacteria) capable of degrading biopolymer or biodegradation by-products yields by other species which were isolated in this
The way artists deal with body in their artistic works has had so many forms and methods, whether as an object for their drawings or as a material to create live artistic performances that relate to the idea of correspondence and interaction between different artistic categories such as: drama, dance, and painting as it is the case of the artist Marina Abramovic who has always used her body as an artistic unit to generate meaning and to perform her lively shows.
To go deeper into her career, our work was divided into 3 sections:
The first section was devoted to follow the main artistic stages that her body had gone through, starting with paintings she performed using concepts based on acting, simulation and nudity and ending wi
A study of taxonomic quality of algae attaching Three concert bridges built on the Tigris River within city of Baghdad including Al-Jadriyah Bridge in Al-Jadriyah area and the Bab Al-Mu'adam Bridge in Al-Shalajiya area, while the third site included Al-Muthanna Bridge in north of Baghdad city, for the period from Autumn 2021 and Winter 2022. The study identified 114 species of 32 Genus in which the predominance of Bacillariophceae (74 species, 14 Genus) Followed by Cyanophyceae (30species, 12 Genus) and 10 species (6 Genus) of Chlorophyceae. The study showed an increase in species of Bacillariophceae, Cyanophyceae which has the ability to secretion gelatinous substances that enable it to stick to solid stand, the number of the larges
... Show MoreBackground: In this work, a fingerprint powder was used to reveal latent fingerprints from different surfaces. This powder was derived from the Date fronds as activated carbon. Methods: In preparing the activated carbon, three parameters were studied: activation time, activation temperature, and impregnation ratio. Fourier Transform Infrared Spectroscopy (FTIR) was used to characterize the prepared Date frond activated carbon (DFAC) as well as the raw material (Date frond plant). Brunauer-Emmett-Teller (BET) was used to measure the specific surface area of DFAC. The surface shape and the element composition of the prepared powder were investigated using (SEM-EDS) analysis. A Central Composite Design (CCD) was employed to determine th
... Show MoreWe need to know the basic facts concerning planning top and bottom limits including any critical levels or the threshold over which the cost would be much higher for land development. Therefore this paper concerned with Baghdad Municipality decision No.2/1004 dated 7/12/2004. The reason behind this decision is the hope to face up at least in the severe housing crisis in the city of Baghdad. This paper attempts to know the attitude of the local community in the general through a field study of people living near such dwelling where third floors are added of. This might indicate any positive or negative effects whether on short or long-term including its effect on the theoretical side including the population growth of Baghdad, the
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A research study in which a methodology for quality indicators that can be adopted to evaluate the educational process in educational institutions within a specific strategy helps individuals in charge of educational education to make appropriate decisions based on accurate and properly approved plans to achieve successful outcomes.
These indicators can be used to judge the quality of educational institutions in order to improve, improve and develop them. This study has concluded the necessity of putting quality into actual application in order to benefit from it in evaluating the future action of Iraqi educational institutions.
The external changes brought about by technology and family life have made the
... Show MoreThis study deals with 3rd-order difference equations with alternating coefficients (NDEAC) with a focus on studying the oscillatory properties of their solutions. An additional sufficient condition can be derived to ensure continuity for the oscillatory solution in equations. An applied example is presented to illustrate the theoretical result obtained.
A successful synthesis of mesoporous Santa Barbara amorphous (SBA-15) with nanoparticle size was attempted using prepared sodium silicate from Iraqi high silica sand. EO20PO70EO20 copolymer was used as a template at highly acidic conditions (pH < 2). The effect of crystallization temperature of (100, 110, 120, and 130 0C), and crystallization time of (24, 48, 72, and 120 h) on surface properties was studied. The experiments were characterized using XRD, FTIR, AFM, BET, and FESEM. The XRD and FTIR tests represent amorphous SBA-15 without any impurities. Decreasing average particle size distribution increased the surface area, and decreased the porosity (pore volume, and pore size) in all experiments. The texture properties were su
... Show MoreGiardia lamblia parasite was isolated from the diarrhea samples of patients with Giardiasis dysentery and was developed in HSP media, four mice groups have been used to find in vivo efficacy of two concentrations (128,256) mg/ml of chlorophorm extracts from Cladophora glomerata algae against Giardia lamblia parasite as compared with (Flagyl) by measuring several biochemical markers as ( GPT and GOT) enzymes ,sodium ,potassium and iron concentration as well as counting the number of parasitic cysts in each mice groups. The results demonstrate that levels of GPTA GOT enzymes have been decreased in mice treated with algal extract. As for the concentration of the Sodium, Potassium and Iron increased in mice treat
... Show MoreThe engagement of pharmacists in research activities is pivotal in the advancement of the pharmacy practice. The study aims to evaluate the confidence and competence of Malaysian hospital pharmacists in conducting clinical and practice-based research.
A cross-sectional study was carried out between September 2019 and April 2020 using an online survey. Pharmacists from eight different hospitals in Malaysia were involved in the study. The survey link was sent to all pharmacists of the included hospitals via email. Data were analysed using SPSS version 25.
A total of 226 pharmacists participated in this study, and their average age was 28 years old. About 82 % of the participants reported that they did not have any previous re
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