petroleum refinery wastewater
priority pollutant Phenols
Solid phase
extraction.
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A solid Phase Extraction (SPE) followed by HPLC-UV method is described for the
simultaneous quantitative determination of nine priority pollutant phenols : Phenol, 2- and
4-Nitrophenol, 2,4-Dimethylphenol, 2-, 2,4-Di-, 2,4,6-Tri-, and Penta- chlorophenol, 4
Chloro-3-methylphenol. The phenols were separated using a C-18 column with UV detector
at wave length of 280nm. The Flow of mobile phase was isocratic consisted of 50:50
Acetonitrile: phosphate buffer pH=7.1, column temperature 45 C°, Flow Rate 0.7 ml/min.
Calibration curves were linear (R2 = 0.9961-0.9995). The RSDs (1.301-5.805)%,
LOD(39.1- 412.4) µg/L, LOQ(118.5-1250.8) µg/L, the Robustness (1.55-4.89),
Ruggedness (2.82-4.00), Repeatability (2.1-4.95), Recoveries%
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