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Synergistic Antibacterial Activity of Epidermin and Staphylolysin LasA against Pathogenic Bacteria
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Antibiotic resistance increment is a major problem for the human society nowadays which encourages the efforts to look for new therapeutic alternatives from natural defenses. Synergistic antibacterial activity of epidermin and staphylolysin LasA A against Staphylococcus aureus (Staph aureus), Escherichia coli (E. coli) and Pseudomonas aeruginosa (Ps. aeruginosa) was evaluated. The antibacterial activities of epidermin from Staphylococcus epidermidis (Staph epidermidis) and Staphylolysin (LasA) from Ps. aeruginosa using the agar well diffusion assay were evaluated, and then using the micro dilution method to evaluate the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). The checkerboard method and fractional inhibitory concentration (FIC) were used to evaluate the combination between epidermin and LasA toward targeted clinical isolates of Staph aureus, E. coli and Ps. aeruginosa. The results revealed a synergistic effect between epidermin and LasA on all clinical isolates growth. The highest MIC and MBC of epidermin were 36.04 µL/mL and 51.73 µL/mL against Staph aureus; meanwhile, the highest MIC and MBC of LasA were 44.38 µL/mL and 50 µL/mL against Staph aureus. The FICindex revealed synergistic interactions in combination of epidermin and LasA which recorded 0.286 for Staph aureus while for E. coli was 0.327 and for Ps. aeruginosa was 0.390 respectively showing a synergism effect. This study finds that combination of epidermin with LasA had inhibitory activity on the targeted clinical isolate growth, which can be useful for designing and developing alternative therapeutic strategies against pathogens causing wound and burn infections.

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Publication Date
Mon Mar 01 2010
Journal Name
Iraqi J Pharm Sci,
Synthesis, Characterization and Antibacterial Activities of Ligand Type N2O4 Schiff base and its Novel Complexes with Co(II), Ni(II), Cu(II) and Zn(II) ions
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The reaction of 2-amino benzoic acid with 1,2-dichloroethane under reflux in methanol and KOH as a base to gave the precursor [H4L]. The precursor under reflux and drops of CH3COOH which reacted with (2mole) from salicycaldehyde in methanol to gave a new type N2O4 ligand [H2L], this ligand was reacted with (MCl2) Where [M= Co (II), Ni(II), Cu(II) and Zn(II)] in (1:1) ratio at reflux in methanol using KOH as a base, to give complexes of the general formula [M(L)]. All compounds have been characterized by spectroscopic methods [1H NMR ( just to the ligand), FTIR, uv-vis, atomic absorption], melting point, conductivity, chloride content, as well as magnetic susceptibility measurements. From the above data, the proposed molecular structu

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Publication Date
Thu Mar 30 2017
Journal Name
Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)
Synthesis, Characterization and Antibacterial Activities of Ligand Type N2O4 Schiff base and its Novel Complexes with Co(II), Ni(II), Cu(II) and Zn(II) ions.
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The reaction of 2-amino benzoic acid with 1,2-dichloroethane under reflux in methanol and KOH as a base to gave the precursor [H4L]. The precursor under reflux and drops of CH3COOH which reacted with (2mole) from salicycaldehyde in methanol to gave a new type N2O4 ligand [H2L], this ligand was reacted with (MCl2) Where [M= Co (II), Ni(II), Cu(II) and Zn(II)] in (1:1) ratio at reflux in methanol using KOH as a base, to give complexes of the general formula [M(L)]. All compounds have been characterized by spectroscopic methods [1H NMR ( just to the ligand), FTIR, uv-vis, atomic absorption], melting point, conductivity, chloride content, as well as m

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Publication Date
Thu Oct 01 2020
Journal Name
Journal Of Engineering Science And Technology
Automatic voice activity detection using fuzzy-neuro classifier
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Voice Activity Detection (VAD) is considered as an important pre-processing step in speech processing systems such as speech enhancement, speech recognition, gender and age identification. VAD helps in reducing the time required to process speech data and to improve final system accuracy by focusing the work on the voiced part of the speech. An automatic technique for VAD using Fuzzy-Neuro technique (FN-AVAD) is presented in this paper. The aim of this work is to alleviate the problem of choosing the best threshold value in traditional VAD methods and achieves automaticity by combining fuzzy clustering and machine learning techniques. Four features are extracted from each speech segment, which are short term energy, zero-crossing rate, auto

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Publication Date
Sat Jun 01 2019
Journal Name
Journal Of Engineering And Applied Sciences
Enhancing LSB algorithm against brute-force attack using ASCII mapping technique
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Publication Date
Thu Jan 04 2018
Journal Name
Journal Of Electrical Engineering And Technology
An efficient selective method for audio watermarking against de-synchronization attacks
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Publication Date
Wed Sep 28 2022
Journal Name
Journal Of The College Of Education For Women
Adapting the Grimms: Going Against Patriarchy in Singh’s Movie Mirror Mirror
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Many cinematic adaptations were produced for the Grimms’ “Little Snow-White” (1812) including Mirror Mirror movie (2012), the contemporary version adapted by Taresm Singh. Singh’s version was able to depict the modern reality of women and went against patriarchy by embracing feminist ideologies of the fourth-wave feminism. Therefore, he challenged the ideologies of the mainstream cinema dominated by the patriarchal élite’s capitalist mode of production that still adhere to the stereotyped patriarchal image of women’s ‘victimization,’ ‘objectification’ and ‘marginalization,’ which did not represent women’s modern reality anymore. This paper, however, is a qualitative study aimed to prove that the femini

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Publication Date
Sun Apr 23 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Synthesis and Characterization of New Schiff Bases Derived from Reaction of The Cefixime with Benzaldehyde Derivatives and Evaluation of Their Biological Activity
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  The Schiff bases (1-10) were synthesized by the reaction of cefixime  with aldehydes derivatives. The characterization of Schiff bases were carried out, by using   spectroscopic techniques including IR, U.V – Vis, H1-NMR, EI-MS along with elemental analyses (C.H.N.).

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Publication Date
Tue Dec 01 2020
Journal Name
International Journal Of Pharmaceutical Research
Synthesis of Some New Schiff Bases Starting from 2,2 Dimorpholinyl Acetic Acid and Evaluating the Biological activity
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n this study new derivatives of Schiff bases (5-10) were synthesized from the new starting material 1 . Which has been synthesized by the reaction of (1 mol.) of dichloroacetic acid with two moles of morpholine, in the presence of potassium hydroxide, Ester derivatives 2 and 3 were synthesized by the reaction of 1 with methanol or ethanol respectively in the presence of sulphuric acid as catalyst . Compound 2 was also prepared from dimethylsulphate with high yield , 2 and 3 was used to synthesized 2,2-dimorpholinylacetohydrazide 4 via reaction with NH2NH2.H2O 80% .Imines (5-10) were synthesized via the reaction of 4 with appropriate aromatic aldehydes in the presence of G.A.A as a catalyst . Derivatives compounds (1-10) were identifie

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Publication Date
Sun May 28 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
The Effect of Watery & Alcoholic Plant Extracts of Elettaria cardamomum On Growth of Some Bacteria Isolated from Otitis media Infections
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This study was done to determine the effect of watery and alcoholic extracts of Elettaria cardamomum  on growth of bacteria isolated from Otitis media Infections which are : Pseudomonas aeruginosa , Staphylococcus  aureus and  Klebsilla   spp.        The biochemical analysis revealed that watery extracts of Elettaria cardamomum  contains: Glycosides,  Alkaloids, Saponines, Tannins, and Volatile oils, ( This compound is Soluble in water ) , while the alcoholic extracts contains : (in addition to the above mentioned compounds ) Phenols , Resins , Flavonids and Coumarins. ( This compound is  Soluble in organic solutions ).      To study the e

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Publication Date
Tue Feb 04 2025
Journal Name
Microbes And Infectious Diseases
A preclinical evaluation of the response of repairing the DNA of MCF7 breast cancer cells after exposure to probiotic bacteria
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