Background: Denture lining materials are widely used in prosthodontic treatment and management of traumatized oral mucosa. A contaminated prosthesis can provide a source of cross-contamination between patients and dental personnel as well as a cause for denture stomatitis. Therefore, denture disinfection has been recommended as an essential procedure for maintenance of a healthy oral mucosa. This study investigated the effect of SOLO disinfectant solution on some properties of different denture lining materials. Materials and methods: Three different solutions were used in this study; SOLO disinfectant solution, sodium hypochlorite solution, and water on three types of acrylic denture lining materials; hot cure, cold cure, and soft acrylic resin. Twenty seven disk-shaped samples were used to evaluate the color stability and forty five rectangular samples were used for testing the surface micro hardness and surface roughness of the different denture lining materials. Data measurements of the color stability, surface hardness, and surface roughness were analyzed and compared statistically. Results: The color stability for the tested denture lining materials was insignificantly affected (p>0.05) by the immersion in the SOLO disinfectant solution. There was a highly significant difference (p< 0.01) in the surface hardness of the hot cure while it was insignificant (p>0.05) for cold cure denture lining materials when immersed in the SOLO disinfectant solution. For surface roughness there was no significant difference (p>0.05) by immersion in SOLO disinfectant solution for the different denture lining materials. Conclusions: Based on the results of this study SOLO disinfectant solution produced no adverse effect on the color stability, surface hardness, and surface roughness of the hot cure, cold cure, and soft acrylic denture lining materials