The hydrolysis of urea by the enzyme urease is significant for increasing the irroles in human pathogenicity, biocementation, soil fertilizer, and subsequently in soil improvement. This study devoted to the isolation of urease from urea-rich soil samples collected from seven different locations. Isolation of the various bacterial species was conducted using nutrient agar. The identity of isolated urease was based on morphological characteristics and standard microbiological and biochemical procedures. The urease producing strains of bacteria were obtained using the urease hydrolysis test. The bacterial isolates produced from soil samples collected from different environments and treated by different morphological processes helped in precipitation of large calcium carbonate (CaCO3) crystal aggregates precipitated within bacterial colonies grown on agar. The different microbial species and functional attributes produced striking differences in the morphology of precipitated crystals. The phylogenetic sequencing of 16S ribosomal RNA genes produced several isolates that are mostly related to the Bacillus group. One strain of promising results was selected and the environmental and nutritional conditions were characterized. The growth curve of the selected strain with an optimized condition was investigated.
A total of (25) stool samples were collected from children and adults (2- 4) years old suffering from diarrhea to isolate E. coli strains that produce heat-stable enterotoxin a (STa), and after performing microscopic examination, cultural characterization and biochemical identification only (11) isolates showed positive E. coli. STa activity was estimated by using suckling mouse assay (SMA) and from these (11) isolates only (5) showed STa activity and the one with the highest STa activity was selected for large scale production of STa, which was followed by partial purification using ion-exchange chromatography (normal phase) using DEAE sephadex A-50 column. After purification and determination of protein concentration by using the standard
... Show MoreThe present study was conducted to estimate the antimicrobial activity and the potential biological control of the killer toxin produced by
Background: Obesity is a worldwide challenge and is closely
connected to many metabolic diseases. Two types of
adipose tissue, white adipose tissue (WAT) and brown
adipose tissue (BAT) have been identified. White fat cells
store chemical energy, brown adipocytes defend against
hypothermia, obesity and diabetes.
Objective: To localize and quantify brown adipocytes in
human subcutaneous (S) and visceral (V) adipose tissue by
histology and immunohistochemistry.
Type of the study: A cross –sectional study.
Methods: Adipose tissue was obtained from histopathology
specimens taken from ten patients, of different age, sex and
body mass index (BMI), undergoing surgery for different
pathologies
Background: Toxin-producing Shiga Escherichia coli has been identified as a new foodborne pathogen that poses a significant health risk to humans. Shiga toxin-producing Escherichia coli can be found in raw cow milk and its derivatives. A small number of Escherichia coli strains that produce shiga toxin are pathogenic. Aim of study: The study aimed to see if there were any virulence genes in 50 milk samples that were typical of Entero-haemorrhagic E. coli and evaluate the Myrtus communis effects on these bacteria. Materials and Method: Milk samples were used to isolate E. coli bacteria (n= 27), biochemically analyzed, and genetically screened for virulence genes using a multiplex (PCR). The hydro-alcoholic extraction of Myrtus communis leave
... Show MoreThe purposes of this study are to measure masculine's identification among secondary school students, and to examine the significant differences among secondary school students in respect of students age (16-18) year. The researcher has prepared a questionnaire comprised (25) item to measure masculine identification among students: this scale was applied on (100) student who were selected as a sample from four different school at al-rusafa side in Baghdad. The scale then modified to (23) item to be more consistent. The results revealed that there were no significant differences among students based on the variable of age, and the mean of masculine identification showed a high level than the hypothesized mean.
This study aims to reach the right of governorates that are not organized in a region to impose local legislation, including tax legislation, and the extent of the constitutionality of this legislation and its consistency with constitutional texts and legal rules. The imposition of local taxes finds its constitutional and legal basis in the Iraqi constitution for the year 2005 and the law of governorates not organized in a region.The imposition of local taxes corresponds to the principle of tax legality, which is reflected in the necessity of issuing tax laws from a competent authority, whether this authority is federal, regional or local. Rather, it is sufficient that it be competent
The local asphalt concrete fracture properties represented by the fracture energy, J-integral, and stress intensity factor are calculated from the results of the three point bending beam test made for pre notches beams specimens with deformation rate of 1.27 mm/min. The results revealed that the stress intensity factor has increased by more than 40% when decreasing the testing temperature 10˚C and increasing the notch depth from 5 to 30mm. The change of asphalt type and content have a limited effect of less than 6%.
The increasing level of residents’ requirements of the local community led to the necessity for sufficient local funding to satisfy the residents’ requirements and services of the local units affiliated with the decentralized administrative systems on the one hand, and to the role of local financing in the financial independence of local units on the other hand.With the presence of local financing, the financial independence of local units is achieved and is considered one of the conditions for financial independence, which is the provision of local financing to the units away from central support. The study focused in this research to clarify the concept of local financing for local units with a statement of its conditions and importan
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