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Berbamine and thymoquinone exert protective effects against immune-mediated liver injury via NF-κB dependent pathway
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Background

Immune-mediated hepatitis is a severe impendence to human health, and no effective treatment is currently available. Therefore, new, safe, low-cost therapies are desperately required. Berbamine (BE), a natural substance obtained primarily from Berberis vulgaris L, is a traditional herbal medicine with several bioactivities, such as antimicrobial and anticancer activities. Thymoquinone (TQ), a phytochemical molecule derived from the Nigella sativa plant's black cumin seeds, has attracted interest owing to itsanti-inflammatory, antioxidant, and anticancer properties.

Aim

This current study's aims was to examine the protective impacts of BE and TQ in Concanavalin A (ConA)- induced acute liver injury and the action's underlying mechanism.

Methods

sixty mice of both sexes were used and divided into four groups (each group with six mice) as follows: Group I obtained distilled water (negative control group). Group II received distilled water with a single dose of 0.1 ml ConA (20 mg/kg) on day 4 by retro-orbital route (model group). Groups III and IV received BE (30 mg/kg/day) and TQ (25 mg/kg/day), respectively, by oral gavage for four successive days, with a single dose of ConA (20 mg/kg) on day 4, then all animals were sacrificed after 8 h and prepared for liver and blood collection.

Results

ConA administration increased the ALT, AST, TNF-α, INFγ, and NF-κB significantly (p < 0.001) in the model group. Both BE and TQ could reduce these parameters significantly (p < 0.001) in groups III and IV, respectively, compared to the model group.

Conclusion

Both BE and TQ prominently attenuated ConA immune-mediated liver injury. These findings give a remarkable insight into developing a new therapeutic agent for treating hepatitis and other autoimmune diseases.

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Sun Mar 12 2017
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Effect of Lallemantia royleana Seeds Extract in Liver Functions Enzymes Levels and in some Mice Activity Features Treated with Rifadin drug
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Wed Apr 01 2015
Journal Name
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Isolation of Candida Spp. from Patients with Different Types of Leukemia Who Suffered Oral Candidiasis Due to Their Weekend Immune System
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Sun Jul 29 2018
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Publication Date
Mon Oct 01 2007
Journal Name
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Genotoxic effects among petrol station workers.
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EFFECTS OF LIVE BODY WEIGHT AND LEPTIN CONCENTRATION ON IRAQI CHICKENS FERTILITY AND HATCHABILITY
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Some Studies on Noise and Its Effects on Industrial/Cognitive Task Performance and Modeling
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Publication Date
Thu Jan 01 2015
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Antimicrobial effect of Trigonella foenum graecum Seed Extracts against Tested Bacteria Isolated from Meat and Fish
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Publication Date
Tue Apr 30 2024
Journal Name
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Role of Arbuscular Mycorrhiza (AM) Fungi and Lignin in Biological Control against Vascular Fusarium Wilt Disease
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