Aim of the present study is Identification of specific gene for GPCR using specific primers .and identification of difference in PCR analysis in patients with heart thrombosis and compared with healthy, Sequencing of PCR product regarding GPCR compared for all three subject, Identification the similarity of human GPCR with local strain of yeast fifty healthy control and fifty patients with thrombosis which diagnosed medically with cardiac specific troponin t, troponin 1 levels and electro myocardiogram ECG. The aged for all subjects ranged (39-75) years patients were lying in cardiac care unit at Ibn- al- Nafees teaching hospital and Sheikh Zayed teaching hospital. Genomic DNA of whole blood was extracted from buffy coat and cell cultured handbook protocol using Bioneer- kit and Genomic DNA fungus/yeast kit was used in isolation and purification of DNA. patients divided into three groups according to their age: group A (60-75) years , group B (50-59) years , group C (39-49) years the results of genomic DNA isolation from blood cells extracted in pure form which ensured by the absorbance ratio (260/280 ) was (1.6 – 1.9 ) with a concentration of 50µg/ml and one DNA band with high resolution in gel electrophoresis. The result of genomic DNA extracted from the local strain of S. cerevisiae showed that DNA extracted with high purity because the absorbance ratio (260 /280 )was (1.7 to 2.0) with a concentration of 60 µg/ml and presence one DNA band with high resolution in gel electrophoresis. primers were designed depending on the sequence of the gene responsible for the production of GPCR on the chromosome 11 , GPCR contain three exons which covered with six primers to detect a defect in gene sequence among. Results of gel electrophoresis are showed that primer GPRX1 gave one band for (Control , A,B ) groups but absent amplified band in the patient eight and nine from group C. with molecular weight of this band is 1000 bp. The GPRX2 primer used to amplify second exon in the GPCR gene ,the molecular weight of amplified bands are 400 bp were present in all control samples and three groups of thrombosis patientsand yeast. GPRX2A primer that designed to amplify part two from second exon of GPCR gene by PCR gave one band for all samples which include control and patient, the molecular weight of this band is 500 bp. PCR analysis showed one amplify band for all control and patients group with molecular weight 500 bp for GPRX3 primer and 400 bp for GPRX3A ,300 bp for GPRX3B. The specific primers which designed to covering GPCR gene used to amplification genomic DNA of the local strain S.cerevisiae by PCR technique. Results showed all six primers which gave one band with difference molecular weight for each primer. All samples demonstrate identity planned sizes to control and local strain of S. cerevisiae samples except patient number 8 and 9 in the group(C) that showed non specialist bands in specific primer with first exon (GPRX1) .So the genetic sequence analysis of these two case based on the sequence of the remainder exons to detect the genetic defect in these case. The sequence of the first part for the second exon (X2) was identity standard sequence found on the NCBI web site for case( 8). The case (9) showed identity with the sequence present in the human gene bank but some difference in the first of sequence which neglected because it is in the place link of primer. The results for case 8 showed some mutation for Exon X2(part2). but case (9) demonstrate one deletion and one substitution. The results, also, illustrated that the ether48 thrombosis patients didn't appeared any mutation despite the positive results for ( Troponin) that gives strong indication of thrombosis. The conclusion Primer GPRX1 gave one band for (Control , A,B ) groups but absent amplified band in the patient eight and nine from group C. The molecular weight of this band is 1000 bp. The amplified band with molecular weight 400 bp were present in all control samples and three groups of thrombosis patients with primer GPRX2 and 500 bp with primer GPRX2A.PCR analysis showed one amplify band for all control and patients group with molecular weight 500 bp for GPRX3 primer.,400 bp with GPRX3A and 300 bp for primer GPRX3B.Similarity between results given by healthy group and local strain of yeast. Genetic study showed that there are only two case of patients eight and nine demonstrated mutation in nucleic location on exon two and three from GPCR gene
The results of the present study showed that twenty-five samples were collected for the age group 35–40 years and four samples for the age group 65–70 years for both genders. The results showed that 48 (48%) of the samples were obtained from the hands, 16 (16%) from the legs, 12 (12%) from the abdominal area, and 10 (10%) from the chest area. The four (4%) samples were obtained from burns in the back and thighs area. The samples taken according to the cause of burns were 40 (40%) due to hot water, hot liquids, or hot steam, followed by 18 (18%) due to the use of hot tools, 15 (15%) due to fires, 12 (12%) due to electric currents, 10 (10%) due to chemicals such as strong acids, alkaline lye, paint thinner, or gasoline, and 5 (5%) due
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The external morphology of planthoppers belong to the genus Dictyophara Germar 1833, has been described. The study included detailed description of the external morphology of Dictyophara iranica Germar 1833. External morphological characters of the head, thorax and their appendages were used. Those characters were supported by studying the male genitalia
In this work, we calculate and analyze the photon emission from quark and anti-quark interaction during annihilation process using simple model depending on phenomenology of quantum chromodynamic theory (QCD). The parameters, which include the running strength coupling, temperature of the system and the critical temperature, carry information regarding photon emission and have a significant impact on the photons yield. The emission of photon from strange interaction with anti-strange is large sensitive to decreases or increases there running strength coupling. The photons emission increases with decreases running strength coupling and vice versa. We introduce the influence of critical temperature on the photon emission rate in order
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Levan is an exopolysaccharide produced by various microorganisms and has a variety of applications. In this research, the aim was to demonstrate the biological activity of levan which produced from B. phenoliresistens KX139300. These were done via study the antioxidant, anti-inflammatory, anticancer and antileishmanial activities in vitro. The antioxidant levan was shown 80.9% activity at 1250 µg/mL concentration. The efficient anti-inflammatory activity of 88% protein inhibition was noticed with levan concentration at 35 µg/mL. The cytotoxic activity of levan at 2500 µg/mL concentration showed a maximum cytotoxic effect on L20B cell line and promastigotes of Leishmani tropica. Levan has dose-dependent anticancer and antileishman
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Nanometal oxides were extracted from cement factories wastes and were employed to proliferate skin cancer cells using MTT (Methyl Thiazolyl Tetraolium) assay. MTT assay results for skin cancer cell line A375,for CaO NPs the best concentration was 200 μg/mL,The viability was reduced to 57.28% while the IC50 was (69.66) g/mL for A375 and normal cell WRL68 was significantly higher (231.2 g/mL). The cytotoxicity results of CaO: MgO: Fe2O3 NPs, at higher concentrations (200 and 400) μg/mL showed a significant difference. The IC50 was (106.4) μg/mL for A375and normal cell WRL68 was significantly higher (173.3) μg/ml CaO:MgO:Fe2O3 NPs. The nanometal oxides, calci
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The root lesion nematode, Pratylenchus neglectus, is one of the most damaging nematodes to affect wheat worldwide. The nematode is widely distributed in Montana, primarily affecting winter wheat within the state. Managing the nematode primarily involves rotation to resistant and moderately resistant crops (peas, lentils, and barley). A nematode survey was conducted across the state nearly 10 years after an initial survey, to reassess the nematode threat and assess the impact of changing trends in crop rotations. To assess the broad applicability of rotation crops to control P. neglectus across Montana, greenhouse trials were conducted to challenge rotational crops using eight populations of P. neglectus collected from geographicall
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The main objectives of this research is to extract essential oil from: orange ( citrus sinensis), lemon( citrus limon) and mandarin( citrus reticulata) peels by two methods: steam distillation (SD) and microwave assisted steam distillation (MASD), study the effect of extraction conditions (weight of the sample, extraction time, and microwave power, citrus peel type) on oil yield and compare the results of the two methods, the resulting essential oil was analyzed by Gas Chromatography (GC).
Essential oils are highly concentrated substances used for their flavor and therapeutic or odoriferous properties, in a wide selection of products such as foods, medicines and cosmetics. Extracti
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