A total of 20 raw milk samples were used as the fouling agent for evaluating the bacteriological effectiveness of cleaning and sanitizing of domestic milking equipment by using ozonated water at 0.5 ppm comparing to the warm water at 55! for 5 minutes respectively. The mean values of total aerobic bacteria, Coliform and E.coli that present on the plastic and stainless-steel containers after using the raw milk as fouling agent were 3.4×10-6 , 6.7x10-5 and 5.8×10-3 cfu/cm2 respectively , after cleaning the stainless steel containers by the ozonated water the mean values of total aerobic bacterial counts, Coliforms and E.coli bacteria were reduced to 1.2×10-6, 4.7×10-5 and 3.3×10-3 CFU/cm2 respectively. while after cleaning by the warm water were only reduced to 2.3x10-6, 2.7×10-5and 2.8×10-3 CFU/cm2 respectively . The mean values of total aerobic bacteria, coliform ,and E.coli counts/ cm2 after cleaning the plastic containers by the ozonated water were reduced to 1.4×10-6, 1.8×10-5 and 2.4×10- 3 CFU/cm2 respectively, while the mean values after cleaning by the warm water were reduced to 1.1×10-6, 1.7×10-5 and 2.3×10- 3 CFU/cm2 respectively .The mean values of total aerobic, Coliforms and E.coli bacteria after using both the Ozonating and warm water for both stainless steel and plastic containers were reduced to 4.2x10-5, 2.1x10-3, 1.6x10-2, 2.2x10-5, 5.1x10-3 and 1.4x10-2 CFU/cm2 respectively. The results of the current study showed that the independed either treatment by the ozonated or warm water not effectively reduced the number of the quality control bacteria because such reduction in bacterial population could not be enough to guarantee the safety of the home dairy containers, while the combined both treatments had a significant reduction in the population of the quality control bacteria. The effectiveness of cleaning and sanitization by the ozonated and warm water process may be depending on the initial count contaminated bacteria of raw milk, exposure time, kind of containers and both concentration of ozone and the heating temperature.
