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Immunological and molecular detection of Helicobacter pylori in patients clinically diagnosed with chronic urticarial and atopic dermatitis
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To determine the relationship between Helicobacter pylori infection and skin disorders, sixty six patients who suffering from skin diseases include chronic urticarial (CU) and atopic dermatitis (AD) who attended at Dermatological Clinic/ Al-Numan Teaching Hospital from the beginning of October 2015 to the end of January 2016 with age (6-62) have been investigated and compared to twenty two samples of apparently healthy individuals were studied as control group. All the studied groups were subjected to measurement of antiHelicobacter pylori IgG antibodies by enzyme linked immuno sorbent assay (ELISA) and detection of 16S rRNA and CagA genes by using singleplex and multiplex PCR methods. The results of current study revealed that there was a highly significant elevation (P<0.01) in concentration of H. pylori IgG antibodies in sera of the CU and AD patients compared with control group, also the results revealed that there was a significant elevation (P<0.05) in concentration of H. pylori IgG antibodies in sera of the CU patients compared with control group, and significant elevation (P<0.05) in concentration of H. pylori IgG antibodies in sera of AD patients compared with control group. The results of present study indicated that 26(66.67%) patients out of 39 CU patients were positive for both of 16S rRNA and CagA genes, while 13(33.33%) patients out of 39 CU patients were negative for those genes. Also, the results revealed that 19(70.37%) patients out of 27 AD patients were positive for both of 16S rRNA and CagA genes, whereas only 8(29.62%) were negative for both those genes comparing with control group which showed 1(4.54%) individuals out of 22 apparently healthy individuals were positive for both 16S rRNA and CagA genes, the statistical analysis was highly significant (P<0.01).

Publication Date
Wed Nov 27 2019
Journal Name
Iraqi Journal Of Science
Assessment of Inflammation, Comorbidity and Demographic Factors in Patients with Kidney Disease in Baghdad
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     Inflammation markers are significantly higher among hemodialysis (HD) patients, which have been associated with chronic activation of the immune system. Hemodialysis centers in Baghdad appear to be taking measures with low adequacy and frequency of dialysis sessions, which can be a reason for decreased kidney functions. Therefore, the objective of this study focuses on the assessment of different aspects of hemodialysis for regular HD patients in Baghdad, including inflammatory markers (serum C-reactive protein, CRP, and erythrocyte sedimentation rate, ESR), dialysis dose, comorbidities, and demographic factors for a period of one year (2018), the assessment covered four major hospitals in Baghdad namely (Al-Kind

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Publication Date
Tue Apr 01 2014
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Comparative Study of Allopurinol and Uralyt-4 in patients with Hyperuricemia in Diyala Governorate.
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Background: Hyperuricemia represents a major public health problem; approximately most population has hyperuricemia, serum uric acid ≥6.5 mg/dL in women and ≥7.0 mg/dL in men.
Allopurinol has been approved for the treatment of hyperuricemia patients. Epidemiological and experimental studies suggest a linkage between hyperuricemia and type of treatment.
Objective: To assess the effect of allopurinol and uralyt- 4 and mixture of two drugs (allopurinol and uralyt-4) in hyperuricemia patients
Methods: A total of 60 serum patients with history of hyperuricemia, obtained from Baladrus Hospital in Diyala governorate, were included in this study. In addition, ten normal uric acid level persons used as contro

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Publication Date
Wed Jan 01 2020
Journal Name
Iop Conference Series: Materials Science And Engineering
Study the synergistic Effect between Nanoparticles and Spiramycin on Immunological Response Against Toxoplasmosis
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Abstract<p>This investigation was carried out to estimate the antiparasitic potential of silver and Chitosan nanoparticles loaded with spiramycin against toxoplasmosis infected. After mice injected intraperitoneal in a dose 10<sup>3</sup>viable tachyzoites for acute infection; then treated with spiramycin, chitosan nanoparticles and silver nanoparticles as a single or combined therapy given for seven days. Peritoneal fluid examination revealed a significant decrease in the number of<italic>T. gondii</italic>tachyzoites in all treated infected mice compared with infected non-treated. The combined therapy presented better results than the single one. The best effect was observed in a group of </p> ... Show More
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Publication Date
Wed Apr 05 2017
Journal Name
International Journal Of Science And Research
Antibacterial Effects of Pomegranate Extract (Ellagic Acid) on Some Clinically Isolated Periodontal Pathogens in Vitro Study
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Publication Date
Tue Jun 20 2023
Journal Name
Baghdad Science Journal
Determination of Molecular Weight and Viscosity of Irradiated Polystyrene
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The varied applications of polystyrene in various fields of life led to examining the cause of radiation influence on some rheological behavior of commercial Polystyrene (PS) solution in the chloroform (CHCl3) solvent. Polystyrene grains shape samples were irradiated using the radioactive element Cesium- 137 with (9 µci) activity for 10, 20, and 30 minutes. The viscosity of the polymer solution depends on the concentration and size (i.e. molecular weight) of the dissolved polymer. Experimental data showed that the radiation dose affected the value of viscosity (shear, relative, specific, and reduced). The viscosity value significantly reduced at 10 min radiation dose and when increasing the dose, the viscosity value increased

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Publication Date
Sun Apr 04 2010
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Molecular diagnosis of bcr-abl fusion gene in CML patients using Monoplex-Two Steps- Reveres Transcriptase–Polymerase Chain
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Background: Chronic myeloid leukemia (CML) is a stem cell disorder associated with an acquired chromosomal abnormality, Philadelphia chromosome (Ph), which arises from the reciprocal translocation of part of long arm of chromosome 9, in which proto-oncogene ablson gene (abl) is located, to long arm of chromosome 22, in which break point cluster region gene (bcr) is located. The bcr-abl fusion gene can be detected using several molecular methods. For its simplicity, rapidity, and sensitivity, Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) is one of the most common techniques used for analyzing whether a target gene is being expressed or not.
Patients and methods: Venous blood (VB) sample from hem

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Publication Date
Sun Apr 03 2011
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Breast lesion during lactation diagnosed by fine needle aspiration cytology
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Background: The breast function as a secretory gland during pregnancy and lactation due to the interaction of estrogen, progesterone and other pituitary hormones.

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Publication Date
Sun Sep 07 2014
Journal Name
Baghdad Science Journal
Detection of RAF fusion transcripts in FFPE samples of Medullablastoma and Ependymom in Iraqi children with RT-RQPCR assays
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Medulloblastomas and ependymomas are the most common malignant brain tumors in children. However genetic abnormalities associated with their development and prognosis remain unclear. Recently two gene fusions, KIAA1549–BRAF and SRGAP3–RAF1 have been detected in a number of brain tumours. We report here our development and validation of RT-RQPCR assays to detect various isoforms of these two fusion genes in formalin fixed paraffin embedded (FFPE) tissues of medulloblastoma and ependymoma. We examined these fusion genes in 44 paediatric brain tumours, 33 medulloblastomas and 11 ependymomas. We detected both fusion transcripts in 8/33, 5/33 SRGAP3 ex10/RAF1 ex10, and 3/33 KIAA1549 ex16/BRAF ex9, meduloblastomas but none in the 11 ep

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Publication Date
Tue Nov 30 2021
Journal Name
Iraqi Journal Of Science
Detection of Chlamydia Pneumoniae in Lung Tissues Derived from Lung Tumor-Bearing Iraqi Patients
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Chlamydia pneumoniae is an intracellular gram-negative bacteria associated with lower and upper respiratory tract infections. Several studies, mostly achieved by serological assays, proposed a role for this bacteria in lung cancer risk. Therefore, this study aimed to evaluate the prevalence of Chlamydia pneuomoniae in fresh lung tissues of a sample of Iraqi patients with lung tumors, utilizing polymerase chain reaction (PCR) technique. . Chlamydia pneumoniae DNA was detected in 86.67% of samples. Besides, DNA sequencing of 16S rRNA gene revealed that our isolate is closely related to Chlamydia pneumoniae TW183 strain. It is concluded that Chlamydia pneumoniae  is found in fresh l

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Publication Date
Wed Nov 30 2022
Journal Name
Iraqi Journal Of Science
Detection and Classification of The Osteoarthritis in Knee Joint Using Transfer Learning with Convolutional Neural Networks (CNNs)
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    Osteoarthritis (OA) is a disease of human joints, especially the knee joint, due to significant weight of the body. This disease leads to rupture and degeneration of parts of the cartilage in the knee joint, which causes severe pain. Diagnosis of this disease can be obtained through X-ray. Deep learning has become a popular solution to medical issues due to its fast progress in recent years. This research aims to design and build a classification system to minimize the burden on doctors and help radiologists to assess the severity of the pain, enable them to make an optimal diagnosis and describe the correct treatment. Deep learning-based approaches, such as Convolution Neural Networks (CNNs), have been used to detect knee OA usin

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