The aim of the research was to prepare Pilates exercises using the barrel ladder apparatus and to identify the effect of Pilates exercises on agility, coordination, and motor sequences of third-year female students in artistic gymnastics. The researcher adopted the experimental method to achieve the objectives of the study and to verify its hypotheses, as it is suitable for the nature and problem of the research. In selecting the research population, the researcher carefully chose the sample using a purposive method, clarifying its elements and constituent units. The research population consisted of third-year female students at the College of Physical Education and Sports Sciences for Women / University of Baghdad, with a total of 20 students distributed across the two classes (A and B). The researcher addressed the equipment, tools, and means used in the study, which included the steps of conducting the research such as implementing the pilot experiment, administering tests, collecting data, and statistically processing the data. The researcher concluded that there is a strong effect of Pilates exercises using the barrel ladder apparatus on both agility and coordination, as well as a strong effect on motor sequences. The study recommended that instructors of artistic gymnastics avoid traditional methods of teaching basic skills directly without focusing on certain fundamental motor abilities required for movement performance, such as agility and coordination. It also emphasized the necessity for physical education teachers in universities and schools to pay attention to fitness levels and the physical components required for the targeted skill before performing basic skills, in addition to considering other aspects such as physical, technical, tactical, and social dimensions.