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A novel method for the degradation of human blood clot by immobilised bromelain using multi-walled carbon nanotube and polyphenol oxidase
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Pathological blood clot in blood vessels, which often leads to cardiovascular diseases, are one of the most common causes of death in humans. Therefore, enzymatic therapy to degrade blood clots is vital. To achieve this goal, bromelain was immobilized and used for the biodegradation of blood clots. Bromelain was extracted from the pineapple fruit pulp (Ananas comosus) and purified by ion exchange chromatography after precipitation with ammonium sulphate (0-80 %), resulting in a yield of 70%, purification fold of 1.42, and a specific activity of 1175 U/mg. Bromelain was covalently immobilized on functionalized multi-walled carbon nanotubes (MWCNT), with an enzyme loading of 71.35%. The results of the characterization of free and immobilized bromelain demonstrated that the optimum pH for free and immobilized bromelain activity was 7.0, while the pH range of stability was from 5.0 to 8.5 and 4.0 to 9.0, respectively. The optimum temperature for free and immobilized bromelain activity was 45ºC, whereas the stability was 15 to 50°C and 15 to 55°C, respectively. The immobilized bromelain activity was decreased after the fifth reuse, and the storage period of the free and immobilized bromelain was decreased after 6 and 123 days, respectively. Casein was the best substrate-free bromelain, and fibrin was the best substrate for immobilized bromelain. The results of the purification of polyphenol oxidases (PPO) from potatoes by ion exchange chromatography gave a yield of about 54 %, a purification fold of 1.27, and a specific activity of 2804 U/mg. The current study showed that the immobilized bromelain can significantly biodegrade human blood clots in vitro, while the PPO enzyme has no significant effect on blood clots.

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Publication Date
Thu Dec 29 2016
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Recovery of High Concentration Hexavallent Irradiated Uranium From Inorganic Fission Products
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  In this study , extraction of  irradiated high concentration hexavallent uranium from fission products ( high gamma radiation ) was carried out using multistages countercurrent continuous technique (mixer setller) , employing this technique requires a designing flow sheet that recover all the amounts of uranium and to minimize its losses in the nuclear waste streams. Due to the several parameters required to reach this design, SEPHIS program which is one of the famous code in this field were used to select the optimum conditions through many theoretical runs  , finally the experimental results give a good assurance in SEPHIS results and its optimum conditions. 

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Publication Date
Sun Apr 30 2023
Journal Name
Iraqi Journal Of Science
Prevalence of Hepatitis E Virus in AL-Muthanna Province (Iraq) Patients
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Hepatitis E (HE) is an inflammation of the liver caused by hepatitis E virus
(HEV) infection. Iraq is one of the Asian countries with high incidence and
prevalence of hepatitis. In this paper, HE prevalence will be determined in Al-
Muthanna province /Iraq. Commercially available Micro-ELISA for marker of
hepatitis E (HEV IgM, Foresight, USA) kit was used to test (270) patients for HEV
IgMantibodies. Also (10) blood samples from normal healthy individuals were used
as normal control in this study. Among the (270) analyzed serum samples, a total of
(72) samples (26.66 %) were found to be positive for anti-HEV IgM antibodies, and
all these patients were tested for confirmatory test at central public health
labora

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Publication Date
Thu Mar 30 2017
Journal Name
Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)
Synthesis of New Cyclic Amines-Linked Metronidazole Derivatives as Possible Prodrugs
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         Certain cyclic amine derivatives of metronidazole via acetate spacer were prepared. Cyclic amines used are piperidine and piperazine to improve the physicochemical properties and reduce some of metronidazole side effects. This is believed to be done by modification of its structural features to get prodrugs with improved properties over that of metronidazole. The present work includes esterification of metronidazole with choroacetic acid, N-alkylation of the cyclic amines by the halogenated ester and characterization of their structures by spectral(UV and IR) and elemental(CHN)analysis.The melting points, degree of solubilities and partition coefficients were also determined. Both metronid

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Publication Date
Tue Jan 01 2019
Journal Name
Technologies And Materials For Renewable Energy, Environment And Sustainability: Tmrees19gr
An analytical study of soil temperature with respect to its salinity
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Publication Date
Sat Mar 18 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Design of Electrostatic immersion Lens Accelerating Operated Under Zero Magnification Conditions
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 Theoretical study computerized has been carried out in electron optics field, to design electrostatic immersion lens , the inverse problem is important method in the design of electrostatic lenses by suggesting an axial electrostatic potential distribution using polynomial function. The paraxial –ray equation is solved to obtain the trajectory particles that satisfy the suggested potential function. In this research, designed immersed lens length L = 10mm operated under zero condition, as it was obtained the electrode shape of this lens solutions using the Laplace equation The results of the search showed low values of spherical and chromatic aberrations, which gives a good indication of the design of the lens.   It was

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Publication Date
Sun Jul 01 1990
Journal Name
Bulletin Of The Iraq Natural History Museum (p-issn: 1017-8678 , E-issn: 2311-9799)
AQUATIC INVERTEBRATE FAUNA OF AN UPLAND RESERVOIR SYSTEM, CO-WICKLOW, IRELAND
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Littoral and benthic invertebrates from Roundwood Reservoir System were sampled. Oligochaetes and molluscs were the dominant organisms in the littoral and benthic areas Trichopterans and chironomids were the most abundant insect groups. Scuba diving samples reinforced that view. Other groups of macroinvertebrates were poorly represented. Vertical and horizontal hauls of zooplankton revealed that there were twelve species of zooplankton present. Daphnia hyalina Leydig and Bosmina coregoni Baird were the two dominant species.

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Publication Date
Wed Nov 28 2018
Journal Name
Iraqi Journal Of Science
Molecular detection of Chlamydia trachomatis infection among males with abnormal semen
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Many studies revealed that Chlamydia trachomatis is the most prevalent bacteria “that cause sexually transmitted infections in the world.. Most of these infections are asymptomatic and there is a remarkable relationship between CT infections and male infertility. Therefore, “the present study is established to determine the effect of Chlamydia trachomatis infection on seminal fluid quality in males suffering from infertility compared with fertile males.. “Semen samples were collected from 63 infertile male and 13 fertile male as control group, attending the clinical laboratory for routine seminal fluid analysis. Seminal fluid was analyzed according to World Health Organization guidelines, the whole geno

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Publication Date
Sat Oct 30 2021
Journal Name
Iraqi Journal Of Science
Role of Kisspeptin Gene Polymorphism in Idiopathic Male Infertility in Iraq
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     This case control study aimed to determine single nucleotide polymorphisms (SNPs) in the Kisspeptin (KISS1) gene in males with idiopathic infertility and their association with sex hormones and semen quality. The study included a total of 60 infertile and 30 healthy fertile males. Our results show that the level of the measured hormones (LH, FSH, Testosterone, Prolactin and Kisspeptin-54) were higher in the control group than in the male infertile group at p<0.05. We used polymerase chain reaction restriction fragment length polymorphism (PCR-RELP) for the genotyping of KISS1 position rs35431622 (Q36R) KISS1, which showed three different genotypes of different sizes; a wild-type homozygous AA of 233 bp and a h

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Publication Date
Fri Jan 20 2023
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Preparation of Polyester/ Micro Eggshell Fillers Composite as Natural Surface Coating
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    To fabricate an inexpensive surface coating with excellent mechanical properties with good water resistance and thermal diffusion, white eggshell fibers with particle size (~1micrometer) has been added by different weight percentages (1,2,3,4,5,6,7 and 8 %) to Unsaturated Polyester.

The weight ratio (4%) of eggshell powder is a good ratio to be added to polyester to improve its mechanical properties, such as hardness, impact strength, and wear resistance. The hardness was improved by (3.75%); impact strength has the same value as polyester, flexural strength by (8.43%) and high improvement in wear resistance (74.4%), as well as to get further improvements in mechanical properties of polyester, the eggshell powder was added

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Publication Date
Fri Sep 25 2020
Journal Name
International Journal Of Drug Delivery Technology
Investigate Retention Behavior of 2-deoxycytidine in Hydrophilic Interaction Liquid Chromatography
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A hydrophilic interaction chromatography has been investigated to separate 2-deoxycytidine chosen for nucleoside. A small molecule with specific features for human serum samples was 2-deoxycytidine tested. 2-deoxycytidine has been applied to self-made stationary hydrophilic phases (ZIC1 and ZIC5). The deoxycytidine (dCD) retention was investigated with varying concentrations of sodium acetate buffer, acetonitrile%, and pH. The results confirmed the hydrophilicity of 2-deoxycytidine. The exchanger retention mechanism was studied taking into account 2-deoxycytidine used for describing the interaction of hydrophilic and cation. For both ZIC1 and ZIC5 exchangers, we described and discussed the influence of chromatographic conditions (co

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