يعتبر "تاج الأشواك" أو نبات شوكة المسيح، وهو من نباتات الزينة الطبية ، ينتمي إلى جنس يوفوربيا. E. milii يحتوي كميات وفيرة من المركبات الفينولية ، التربينات، الستيرويدات والقلويدات. كانت الأهداف الرئيسية لهذه الدراسة هي فحص مستخلصات الفلافونويد والنانو فلافونويد ضد نوعين من خطوط الخلايا السرطانية. تم تصنيع مركبات الفلافونويد النانوية عن طريق تفاعل مركب الكيتوسان والماليك اسد. تم تحليل مركبات الفلافونويد النانوية بواسطة مقياس الطيف الضوئي UV-sp -8001 بطول موجي 200-1000 نانومتر ، تم استخدام المجهر الإلكتروني (TEM) والمجهري الإلكتروني الماسح(SEM) لتحديد الخصائص المورفولوجية لمركبات الفلافونويد النانوية. يعتبر الفلافونويد والنانوفلافونويدعقار قوي ومتطور ضد خلايا سرطان الثدي (MCF-7) وخلايا سرطان البروستات(PC3) . تم فحص الفعالية المضادة للسرطان من مركبات الفلافونويد والنانوفلافونويد على خطين مختلفين من الخلايا السرطانية وكذلك خطوط الخلايا السليمة باستخدام اختبار MTT. تم استخدام تجزئة الحمض النووي وتقنية تلوين الخلايا بالصبغة المزدوجة AO / EtBr لفحص علامات موت الخلايا المبرمج. لتحديد تشتت دورة الخلية ، تم استخدام قياس التدفق الخلوي ، وأظهرت النتائج أن كميات الفلافونويد والنانوفلافونويد لها نشاطا ساما وفعالا ضد خطوط خلايا سرطان الثدي والبروستات. بالإضافة إلى ذلك ، تم ربط إيقاف دورة الخلية في مرحلة G0 / G1 بموت الخلايا المبرمج لخطوط الخلايا التي يسببها الفلافونويد والنانوفلافونويد. وفقًا لهذه النتائج ، تمنع مركبات الفلافونويد والنانوفلافونويد من تكاثر خطوط الخلايا السرطانية ، مما يؤدي إلى توقف دورة الخلية وتحفيز موت الخلايا المبرمج. تشير النتائج المتاحة إلى أن مركب الفلافونويد والنانوفلافونويد سيمثل نهجًا علاجيًا واعدًا لعلاج الخلايا السرطانية من الأنواع الأخرى.
This study aimed for isolation and identification of Candida glabrata and identifying some virulence factors. The distribution of patients with candidemia thrush showed that the age group 50-65 years old recorded the highest incidence of candidiasis in female and male with leukemia by 50% and 37.9 % respectively compared to the lowest incidence of candidiasis in the age group under 17 years old in female and male 8.8% and 13.5%, respectively. While the age group between 5-8 years was high, reaching 18 cases of oral candidiasis and 42% of children with leukemia compared with the age group, which was the least, that reached 9 cases, 21%. The highest incidence of C. glabrata was 59 isolates of females and males with leukemia, while C. kefyer w
... Show MoreCampsis grandiflora (Bignoniaceae) is a fast growing deciduous climber, the dried flowers have been used as a carminative, blood tonic, and febrifuge in Chinese traditional medicine. This plant has an anti-inflammatory, anti-oxidant, anti-depressant, and anti-bacterial effect; with a beneficial role in stagnant blood and endometriosis conditions. In this study, the detection of beta-sitosterol in the hexane extract of Iraqi C.grandiflora flowers was performed using thin layer chromatography (TLC) and high performance liquid chromatography(HPLC); while the isolation done by preparative layer chromatography then structure elucidation of isolated compound was done by FTIR and 1HNMR. Furthermore, assessment of th
... Show MoreThis study was conducted to describe a protocol for the callus establishing culture of Lavandula angustifolia plant and estimating their content of volatile oil. The quantity of volatile oil callus tissues was compared with that of leaves production. Callus was induced from leaf explants on Murashige and Skoog medium (MS) supplemented with Naphthalene acetic acid (NAA) and Benzyl adenine (BA) in different concentrations. Maximum callus fresh weight was obtained in the combination of 10 mg/L BA and 3 mg/L NAA which reached 18 g after four weeks. The results of this work showed that the quantity of volatile oil from the highest fresh weight callus was 6 ml compared with quantity of 18g of leaves which gave 0.5 ml. Volatile o
... Show MoreThis study included isolation of some active materials from Curcuma longa such as tannins, saponins and volatile oils with percentage of 59%, 31%, and 9% respectively. Also the study included the determination of minerals in Curcuma longa such as " Na, Ca and K" using Flame photometer. The concentrations of these minerals were (14 ppm),(10 ppm) and )76 ppm) respectively. The anti-bacterial activity study was performed for the active materials isolated from Curcuma longa against two genus of pathogenic bacteria, Escherichia Coli and Staphylococcus aurous by using agar-well diffusion method. It appeared from this study that all of the extraction have inhibitory effect on bacteria was used. The inhibition zone diameter varies with
... Show MoreThe present study aimed to assess the antibacterial activity of peanut (Arachis hypogaea L.) skin extracts. The phytochemical analysis of the peanut skin extracts was investigated, the result showed a strong presence of flavonoids, phenols, alkaloids and tannins in methanol and ethyl acetate extracts. Antibiotic susceptibility of the bacterial isolates was performed on seven antibiotics represented by Amikacin, Tetracycline, Ciprofloxacin, Chloramphenicol, Ticarcillin, Cefotaxime and Gentamicin by disc diffusion method. The antibiogram for studied isolates revealed high level resistance of A. baumannii to all of the antibiotics under test except amikacin, while Staph. aurous was resistance to Chloramphenicol and Cefotxime and sensitive to A
... Show MoreBackground: Fruits and their by-products are the primary sources of bioactive chemicals in plants. Because of its phytochemical richness, Annona squamosa fruits have gained the alertness of people willing in health-promoting diets. The purpose of this in vitro study was to evaluate the cytocompatibility effect of ethanolic crude extract of Annona squamosa pulp against a human normal cell line as a mouthwash for children. Material and method: The ethanolic extract of Annona squamosa pulp was extracted using the ultrasonic method and then lyophilized to make it powder. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) test was performed to investigate the cytotoxic activity of the pulp extract on a human normal cell l
... Show MoreHumanoids or bipedal robots are other kinds of robots that have legs. The balance of humanoids is the general problem in these types when the other in the support phase and the leg in the swing phase. In this work, the walking pattern generation is studied by MATLAB for two types of degrees of freedom, 10 and 17 degrees of freedom. Besides, the KHR-2HV simulation model is used to simulate the experimental results by Webots. Similarly, Arduino and LOBOT LSC microcontrollers are used to program the bipedal robot. After the several methods for programming the bipedal robot by Arduino microcontroller, LOBOT LSC-32 driver model is the better than PCA 96685 Driver-16 channel servo driver for programming the bipedal walking rob
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