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Isolation and Identification of Flavonoid Compounds from Euphorbia Milii Plant Cultivated in Iraq and Evaluation of its Genetic Effects on Two Types of Cancer Cell Line
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يعتبر "تاج الأشواك" أو نبات شوكة المسيح، وهو من نباتات الزينة الطبية ، ينتمي إلى جنس يوفوربيا. E. milii يحتوي كميات وفيرة من المركبات الفينولية ، التربينات، الستيرويدات والقلويدات. كانت الأهداف الرئيسية لهذه الدراسة هي فحص مستخلصات الفلافونويد والنانو فلافونويد ضد نوعين من خطوط الخلايا السرطانية. تم تصنيع مركبات الفلافونويد النانوية عن طريق تفاعل مركب الكيتوسان والماليك اسد. تم تحليل مركبات الفلافونويد النانوية بواسطة مقياس الطيف الضوئي UV-sp -8001 بطول موجي 200-1000 نانومتر ، تم استخدام المجهر الإلكتروني (TEM) والمجهري الإلكتروني الماسح(SEM) لتحديد الخصائص المورفولوجية لمركبات الفلافونويد النانوية. يعتبر الفلافونويد والنانوفلافونويدعقار قوي ومتطور ضد خلايا سرطان الثدي (MCF-7) وخلايا سرطان البروستات(PC3) . تم فحص الفعالية المضادة للسرطان من مركبات الفلافونويد والنانوفلافونويد على خطين مختلفين من الخلايا السرطانية وكذلك خطوط الخلايا السليمة باستخدام اختبار MTT. تم استخدام تجزئة الحمض النووي وتقنية تلوين الخلايا بالصبغة المزدوجة AO / EtBr  لفحص علامات موت الخلايا المبرمج. لتحديد تشتت دورة الخلية ، تم استخدام قياس التدفق الخلوي ، وأظهرت النتائج  أن كميات الفلافونويد والنانوفلافونويد لها نشاطا ساما وفعالا ضد خطوط خلايا سرطان الثدي والبروستات. بالإضافة إلى ذلك ، تم ربط إيقاف دورة الخلية في مرحلة G0 / G1 بموت الخلايا المبرمج لخطوط الخلايا التي يسببها الفلافونويد والنانوفلافونويد. وفقًا لهذه النتائج ، تمنع مركبات الفلافونويد والنانوفلافونويد من تكاثر خطوط الخلايا السرطانية ، مما يؤدي إلى توقف دورة الخلية وتحفيز موت الخلايا المبرمج. تشير النتائج المتاحة إلى أن مركب الفلافونويد والنانوفلافونويد سيمثل نهجًا علاجيًا واعدًا لعلاج الخلايا السرطانية من الأنواع الأخرى.

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Publication Date
Tue Jun 20 2023
Journal Name
Bulletin Of The Iraq Natural History Museum
IDENTIFICATION OF HARD TICKS FROM BUFFALO BUBALUS BUBALIS (LINNAEUS, 1758) IN IRAQ
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Ticks (Acari: Ixodidae) are ectoparasites that infest livestock in every geographic region of the world and are vectors of several viral, bacterial, and protozoan pathogens to both animals and humans. There is little information is available is about tick presence in Buffalo Bubalus bubalis (Linnaeus, 1758) (Artiodactyla, Bovidae) in Iraq. The current study determined the species of ticks parasitizing Buffalo in some central and southern regions included: Baghdad (Al Fathelia), Karbala (Al-Hussainia), Wasit (Kut and Al-Suwairah), Al-Qadisia (Al- Diwaniyah, Al- Saniya, Al-Mihnawea, and Afak), Thi Qar (Al-Nasiriyah and Al-chibayish), Missan (Amara and Qalaat Salih) and Basrah (Al-Haretha, Al-Madena and Al-Deer). A total of 150 Buffal

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Publication Date
Sat Jan 20 2024
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Isolation and Identification of Endophytic Fungi from Aloe vera Leaves and Chemical Analysis of the Alcoholic Extract of the Leaves Using HPLC, GC, and GC-Mass Devices
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The study's goals were to separate and identify endophytic fungi from Aloe vera leaves by looking at their morphology and molecules, as well as to find the chemical compounds in the leaf extract by using HPLC, GC, and GC-Mass instruments. The results showed that 53 endophytic fungi were isolated from a total of 120 pieces of A. vera leaves, with a total colonization rate of 44.16%. The fungus Aspergillus terreus had a colonization rate of 14.16%; Aspergillus niger had a colonization rate of 13.33%; Penicillium chermesinum demonstrated a colonization rate of 6.66%; Paecilomyces variotii had a colonization rate of 2.5%; Talaromyces radicus; and Aspergillus flavus achie

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Publication Date
Sun Jun 11 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Isolation and Partial Purification of Cell wall Lipopolysaccharides of Pseudomonas Aeruginosa and Using It as Vaccine
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This study ,the samples were   collected from "118 patients " suffering from burn wound contaminated with Pseudomonas aeruginosa and 100 health individuals (male and female ) as a control group ,the samples were wound swap and blood sample  .       Chromatography technique was employed to extract and purify cell wall containing lipopolysaccharide by using P. aeruginosa  isolate ATCC 15692,the purification done by addition of ammonuium sulfate, sodium dodecyl sulfat (SDS) anddialysis, gel filtration chromatography by using sepharose-4B.       Immunogenicity of  LPS component was determined by mice injection under the skin  ,then Ab concentration agai

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Publication Date
Sun Aug 01 2021
Journal Name
Biochemical And Cellular Archeves
ABILITY OF CRONOBACTER SAKAZAKII FOR ADHESION AND INVASION TO SKG-GT-4 CELL LINE
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ABSTRACT : Fifteenth isolates of C. sakazakii were obtained from previous studies of the sample (infant formula, cerebrospinal fluid and blood). All isolates C. sakazakii identification based on microscopic, biochemical test and confirmed by 16SrRNA. We studied the movement of all isolates and study adhesion to polystyrene plate, adhesion and invasion to Esophageal adenocarcinoma (SKG-GT-4) for four isolates [Cerebrospinal fluid (CSF5), Bloods (B 1), Dialak (A1c), Novolac Allernova (C1)] and its cytotoxicity. Results showed that all isolates can move after 4 hours of incubation and increased after 8 hours, the isolates moved to different distances strong, medium, and weak. The results showed that the number of C. sakazakii colony adherent t

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Publication Date
Mon Mar 08 2021
Journal Name
Baghdad Science Journal
STUDY OF EMBARGO’S AFFECT ON CANCER IN IRAQ
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different ?? ? injury ? This study aims to knowing the affect of embargo on cancer tutors in Iraq according to different body systems , In addition, this '?0 kinds study aims at knowing t^e categories ages that can be mostly injured by the cancer Egression analysis and descriptive statistics( median and frequency tables). ^?^???? have been used to achieve these two aims .This study includes ah the seventy cancer s Iraq from 1980-1998 and the data have been from the Ministry of Health / ?? tumors Iraqicancer board administration / central registry. The results of this study are emale productive? : Embargo has affected the ten different body systems as .? central nervous system and opthamamology , Hematology ,Respiratory ? system system , mal

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Publication Date
Tue Aug 07 2018
Journal Name
Indian Journal Of Natural Sciences
Evaluation of Drinking Water Quality in Al Wathba Treatment Plant in Baghdad City-Iraq
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Publication Date
Wed Sep 01 2021
Journal Name
Https://www.researchgate.net/journal/university-of-baghdad-engineering-journal-1726-4073
Evaluation of Drinking Water Quality in Al Wahda Treatment Plant in Baghdad City- Iraq
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This study aims to evaluate drinking water quality at the Al Wahda plant (WTP) in Baghdad city. A conventional water treatment plant with an average flow rate of 72.82 MLD. Water samples were taken from the influent and effluent of the treatment plant and analyzed for some physicochemical and biological parameters during the period from June to November 2020. The results of the evaluation indicate that treated water has almost the same characteristics as raw water; in other terms, the plant units do not remove pollutants as efficiently as intended. Based on this, the station appears to be nothing more than a series of water passage units. However, apart from Total dissolved solids, the mean values of all parameters in the study were

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Publication Date
Wed Sep 01 2021
Journal Name
Journal Of Engineering
Evaluation of Drinking Water Quality in Al Wahda Treatment Plant in Baghdad City- Iraq
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This study aims to evaluate drinking water quality at the Al Wahda plant (WTP) in Baghdad city. A conventional water treatment plant with an average flow rate of 72.82 MLD. Water samples were taken from the influent and effluent of the treatment plant and analyzed for some physicochemical and biological parameters during the period from June to November 2020. The results of the evaluation indicate that treated water has almost the same characteristics as raw water; in other terms, the plant units do not remove pollutants as efficiently as intended. Based on this, the station appears to be nothing more than a series of water passage units. However, apart from Total dissolved solids, the mean values of all parameters in th

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Publication Date
Sun Mar 17 2019
Journal Name
Baghdad Science Journal
Toxic Effects of Purified Microcystins from Soil Blue-Green Alga Oscillatoria pseudogeminata on Tomato Plant Lycopersicon esculentum
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The current study included the isolation, purification and cultivation of blue-green alga Oscillatoria pseudogeminata G.Schmidle from soil using the BG-11liquid culture medium for 60 days of cultivation. The growth constant (k) and generation time (G) were measured which (K=0.144) and (G=2.09 days).

Microcystins were purified and determined qualitatively and quantitatively from this alga by using the technique of enzyme linked immunosorbent assay (Elisa Kits). The alga showed the ability to produce microcystins in concentration reached 1.47 µg/L for each 50 mg DW. Tomato plants (Lycopersicon esculentum) aged two months were irrigated with three concentrations of purified microcystins 0.5 , 3.0 and 6.0

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Publication Date
Mon Jun 28 2021
Journal Name
Journal Of Engineering
The Catholyte Effects on The Microbial Desalination Cell Performance of Desalination and Power Generation
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A microbial desalination cell (MDC) is a new approach to bioelectrochemical systems. It provides a more sustainable way to electrical power production, saltwater desalination, and wastewater treatment at the same time. This study examined three operation modes of the MDC: chemical cathode, air cathode, and biocathode MDC, to give clear sight of this system's performance. The experimental work results for these three modes were recorded as power densities generation, saltwater desalination rates, and COD removal percentages. For the chemical cathode MDC, the power density was 96.8 mW/m2, the desalination rate was 84.08 ppm/hr, and the COD removal percentage was 95.94%. The air cathode MDC results were different

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