Lipase was extracted by Sodium acetate buffer (pH=6; 0.05M) Containing 0.1M NaCl. Enzyme content of crude extract was concentrated by gradual addition of ammonium sulfate at 30-60% saturation. The dialyzed extract was purified on ion-exchange chromatography through DEAE–Cellulose and gel-filtration chromatography through sephacryl S-200 column. The specific activity, enzyme yield and fold purification were 54.06 unit/mg, 42.6% and 10.88 respectively. The molecular weight of the Lipase was 43.651 KDa as determined by gel-filtration chromatography through sephacryl S-200 column. Partial purified lipase used for the improvement of the flavor of butter fat after 12 hours for storage.

This study was carried out to assess genetic diversity of ten cultivars of Rice (Oryza sativa L.). One of DNA markers based on Polymerase Chain Reaction (PCR) was used namely DAF markers (DNA Amplification Fingerprint). Six primers were tested, the results showed, that no amplification products using the primers OPD.14 and OPM.5. Two primers (OPX.8 and OPT.2) produced monomorphic band across all cultivars, while only two primers generated polymorphic bands. The number of total bands produced from one of them (OPN.7) were sixteen. Also this primer produced ten polymorphic profiles (DAF patterns) which were unique to the ten cultivars that could be distinguished. The number of total bands generated by primer OPX.1 were thirteen and this prim

Background Tinea versicolor is a common dermatological problem worldwide in distribution, caused by dimorphic fungus called Malassezia furfur, they exist at the very interface between
commensal and pathogen and, as such, their interaction with the human immune system is of great interest. Immuno-inflamatory activity mediated by different cytokines could have a role in the etiology of tinea versicolor.
Patients and Method: The study covered 46 subjects .It comprised 26 patients with TV compared with 20 healthy normal controls. Serum IL-6 analysis was done by ELISA.
Results: The level of IL-6 was elevated in patients with tinea versicolor, when compared with healthy control
Conclusion: IL-6 increases du

Herpes simplex virus (HSV) is a common human pathogen that causes severe infections in newborns and immunocompromised patients. Conjunctivitis or corneal epithelial keratitis is caused by HSV type 1 all over the world and at all times of the year. The present study was aimed at detecting HSV in patients suffering from conjunctivitis. One hundred and ten (110) clinical samples (90 patients and 20 controls, both males and females) of eye conjunctiva swabs were collected from patients of different ages. The samples were analyzed using qPCR and ELISA techniques. The qPCR results revealed that HSV was present in 47 (52.2%) of the 90 patients who were infected. Of these patients, 25 (48.0%) were males and 22 (57.8%) were females, indicati

Background: Osteoarthritis is a complicated, chronic disorder of cartilage and bone, associated with homeostasis of bio-elements. The current study aims to assess the role of serum progranulin levels among Iraqi patients with knee osteoarthritis. Patients and Methods: The study encompassed 50 patients aged 52.50 ± 3.12 years (25 males and 25 females), admitted to the at the Baghdad Medical City through the period from November 2021 to March 2022. All individuals were identified by physicians in a Rheumatology and Rehabilitation Outpatient Clinic and the clinical data was collected along with the assess¬ment of biochemical parameters. Fasting serum glucose, lipid profile, calcium, magnesium, alkaline phosphatase, vitamin D3, and p

!'hi_,  i1rycsligation  was  carried   ou1 dn J)Ct'iphcral  blom.l s_amplc:s.

wl1ich wendrawi·1  ih)rl1 patients  w.ith  l)1).hoid !'ever. Fifteen  palic111 nging ]5- 45 years old .<iS vvdl as ten sample::.  w:cr: c·ollec ted from healthy   persons-al     the  same   range  of   age.   Sera   were   used   t'or csti niation   the  act i vity  and   sp ci fie  activity  of  t\LJ.A.  The   resuJts sho:«"'d            sig11ltl'c u1t  increase i11&