Background: Stroke defined by diminished cerebral blood flow, results in brain damage and neurological impairments. It often leads to considerable difficulties, such as limited mobility and compromised hand function, usually manifesting as a weakening in the ability to open and shut the hand. Objective: The study evaluates the differences between opening and closing hands when utilizing a soft robot. Methods: The study was conducted in the Mosul Specialist Rehabilitation Center which involved 68 participants, all over 20 years old, with visual impairments. The Iranian Clinical Trials Registry and Ministry of Planning approved the study, The Fugl-Meyer Assessment for Upper Extremity Action Research Arm Test, and Arm Motor Ability Test were used to assess finger extension and flexion. Results: A study revealed that stroke patients who utilized a soft robotic glove experienced notable improvements in hand functionality. Average hand closure scores increased significantly, from 8.14 to 20.36 out of 21 points. Women demonstrated greater progress in hand opening rates compared to men, with an average improvement of 1.91 times between pre- and post-assessments. In contrast, the control group exhibited similar hand-opening advancements across genders. Patients with both ischemic and hemorrhagic strokes showed enhanced abilities in opening and closing their hands. Additionally, the study identified a statistically significant improvement in hand function improvements based on stroke type. Conclusion: The study found that stroke patients using a soft robotic glove showed significant improvements in hand-closing abilities, while exercise had minimal impact. Women showed an average increase in hand opening rate. Both male and female patients showed improvement, with hemorrhagic strokes showing remarkable advancements.