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Ex vivo study of anti-leishmanial activity of artemisinin against Leishmania tropica amastigote
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Leishmania parasites are the causative agent of leishmaniasis. Many studies are inspecting chemical drugs, including the use of miltefosine and amphotericin B, but curative values may be limited for these drugs with side effects due to the chemical origin, therefore, investigating less toxic therapies is essential. The aim of this study was to investigate the effectiveness of artemisinin on Iraqi strain of Leishmania tropica, by experimental macrophage ex vivo infection of amastigotes into mouse macrophage cell-line RAW264.7. Different concentrations (100, 200, 300, 400, 500)μM of artemisinin (ART) were screened to examine the susceptibility of L. tropica amastigotes to invade macrophage cell line along three times of follow up (24, 48 and 72) hours. Results showed that artemisinin had a cytotoxic effect on the parasite in which a significance difference (P < 0.05) in cell viability was observed and IC50 was calculated as 182.6 μM after 48 hours treatment. In addition, percentage of infectivity of intracellular amastigotes was significantly decreased. These findings revealed the potential efficacy of artemisinin against the infectious amastigotes and can be further studied to screen its effectiveness in vivo for exploring a safer herbal compound to treat cutaneous leishmaniasis.

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Publication Date
Mon Jan 01 2024
Journal Name
Brazilian Dental Science
Antimicrobial activity of Er,Cr:YSGG and Ultrasonic on E. faecalis biofilm in the mesial root canal systems of lower molars
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Publication Date
Tue Jun 01 2021
Journal Name
Baghdad Science Journal
The Influence of Different Light Wavelengths on Growth, Enzymes Activity and Photosynthesis of the Marine Microalga Dunaliella parva W.Lerche 1937
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Light is an important factor that influences the growth and photosynthetic efficiency of microalgae; however, little is known about how light intensity together with the wavelength affect the photosynthetic capacity and growth of marine microalgae. In the present study, the growth of the marine green microalga Dunaliella parva was studied and optimized under different light intensities (25 ~ 70 μmol m-2 s-1) and qualities (blue, green, and red) in comparison with white light at 40 μmol m-2 s-1 as a control. The growth was monitored by counting the cell number, pigment content, Chl a, Chl b, and carotenoids concentrations. The optimal growth and highest photosyntheti

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Publication Date
Fri Jan 06 2023
Journal Name
Journal Of Advanced Biotechnology And Experimental Therapeutics
Evaluation of oxidative stress activity and the levels of homocysteine, vitamin B12, and DNA methylation among women with breast cancer
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Breast cancer (BC) is the most common malignant tumor in women and the leading cause of cancer deaths worldwide. This work was conducted to estimate the roles of oxidative stress, vitamin B12, homocysteine (HCY), and DNA methylation in BC disease progression. Sixty BC patients (age range 33–80 years) and 30 healthy controls were recruited for this study. Patients with BC were split to group 1 consisted of stage II BC women (low level), and group 2 consisted of patients in stages III and IV (high level). Malondialdehyde (MDA), glutathione peroxidase 3 (GPX3), HCY, and vitamin B12 levels in the study groups were measured. Also, the 5-methylcytosine (5mC) global DNA methylation levels were evaluated. The results showed a significant

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Publication Date
Fri Apr 01 2022
Journal Name
Baghdad Science Journal
Effect of different levels and sizes of walnut seed residues on phosphorus availability and alkaline phosphatase activity in calcareous soil
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A laboratory experiment has been carried out in the College of Science-University of Salahaddin to study the effect of different levels (0,5,10 and 15%) and sizes(250 and 1000µm) of walnut seeds residues and (160mg.kg-1) phosphorus fertilization on the concentration of phosphorus availability and alkaline phosphatase activity in calcareous soil during 15 and 30 days period of incubation, the experimental design in factorial complet randomize design (C.R.D) with three replications. The results indicated that the application of different levels of walnut seed residues decreases the concentration of phosphorus availability and alkaline phosphatase activity, however the results revealed that combination between levels and sizes o

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Publication Date
Sun Feb 10 2019
Journal Name
Iraqi Journal Of Physics
Preparation of TiO2 nanorods by Sol–Gel template method and measured its photo- catalytic activity for degradation of methyl orange
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Titanium dioxide nanorods have been prepared by sol-gel template
method. The structural and surface morphology of the TiO2 nanorods was
investigated by X-ray diffraction (XRD) and atomic force microscopy
(AFM), it was found that the nanorods produced were anatase TiO2 phase.
The photocatalytic activity of the TiO2 nanorods was evaluated by the
photo degradation of methyl orange (MO). The relatively higher
degradation efficiency for MO (D%=78.2) was obtained after 6h of exposed
to UV irradiation.

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Publication Date
Thu Jul 01 2021
Journal Name
Iraqi Journal Of Science
The Efficiency of Apple Vinegar as A Solvent in Comparison to Water and Ethanol for The Extraction of Some Plants used Against Candida Spp. Biofilm Formation
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Apple vinegar has many uses that include burn and wound healing and as an antimicrobial agent against different microorganisms, but not as a solvent. Therefore, this study aimed to use commercial apple vinegar as solvent to the plants of  roselle (Hibiscus sabdariffa), green tea (Camellia sinensis), and clove (Syzygium aromaticum). The effects of apple-vinegar extracts of these plants were compared with those of aqueous and ethanolic extracts against biofilm formation by Candida genus. Clove vinegar extract demonstrated antibiofilm activity against C. albicans, alone (2.4907± 0.382) or in combination with the antifungal agents fluconazole (1.689±0.33), nystati

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Publication Date
Thu Dec 20 2018
Journal Name
Iraqi Journal Of Market Research And Consumer Protection
ANTIMICROBIAL ACTIVITY OF EDIBLE FILM FROM WHEY PROTIEN ISOLATE INCORPORTED WITH GREEN TEA EXTRACT AND ITS USE IN CHEESE COATIN: ANTIMICROBIAL ACTIVITY OF EDIBLE FILM FROM WHEY PROTIEN ISOLATE INCORPORTED WITH GREEN TEA EXTRACT AND ITS USE IN CHEESE COATIN
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The aim of the current research is to study the effect of adding green tea to the edible film prepared from the whey protein isolate on the effectiveness of microorganisms and evaluating the of antimicrobial effectiveness of these films on Iraqi soft cheese packaging during the nine days of storage as an alternative to commercial packaging. At the beginning of the study, the minimum inhibitory concentration was measured by calculate the diameter of the zone of inhibition on growth of the bacteria and it's included the group of Gram negative bacteria (Escherichia coli, Salmonella spp, Pseudomonas Aeruginosa) and the group of Gram positive bacteria (Staphylococcus Aureus, Bacillus spp) and a yeast (Candida Albican). Where the diameter of t

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Publication Date
Thu Apr 25 2019
Journal Name
Iraqi Journal Of Market Research And Consumer Protection
BIOLOGICAL ACTIVITY OF THE CAROTENOIDS WHICH ARE PRODUCED FROM LOCAL ISOLATE Rhodotorula mucilaginosa AND DETRERMINATION THE CONDITIONS AFFECTING THEIR STABILITY: BIOLOGICAL ACTIVITY OF THE CAROTENOIDS WHICH ARE PRODUCED FROM LOCAL ISOLATE Rhodotorula mucilaginosa AND DETRERMINATION THE CONDITIONS AFFECTING THEIR STABILITY
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Biological activity of the carotenoids which are produced fromchemically-mutaed local isolate of Rhodotorula mucilaginosawas studied. The results showed variation of inhibition activity of caritenoids against different types of pathogenic bacteria include, Staph aureus, E. coli, B. subtilis and Salmo. typh., the number declined from 2×107cell/ml to 2×104, 5×104, 2×105, 9×105 cell/ml respectively after 24hour. The produced carotenoids from alocal mutant Rhodotorula mucilaginosa revealed an antioxidant activity as free radical removal of 85.6%. Carotinoides revealed a highest stability in petroleum ether solvent for 30 days at room temperature. It found that the pigment was more stability in sesame oil compared with sun flower and coc

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Publication Date
Fri Jul 01 2022
Journal Name
Iraqi Journal Of Science
Radionuclides Activity and Radiological Hazard Assessment in Bananas Samples Exported to Iraq
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The average concentration of radionuclide’s in the various banana samples were collected from being the most popular markets in Baghdad city - Iraq were measured by use Sodium Iodide Thallium [NaI(Tl)] detector. The radionuclide’s observed with reliable regularity belonged to the series-decay naturally occurring radionuclide’s headed by 232U and 232Th as well as the non-series nuclide, 40K and 137Cs. The average concentration of 40K was found the least average concentration value recorded as876.74625Bq kg-1.The average concentration of 238U was 28.6425Bqkg-1for ²³²Th, it was found to have the average concentration 14.1805 Bqkg-1. The cesium concentration in the sample was found in the healthy range.

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Publication Date
Thu Nov 09 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Platelet Activity And Some Immunological Findings In Atherosclerosis and Coronary Heart Disease
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The   platelet  activity  and  some  immunological  aspects  of

coronary heart disease CIID were investigated by employing platelet count and serum levels of IgG,IgM and lgA,C3 and C4.Three groups of  patients were investigated : patients with ischemic heart disease IHD,  patients  with      lliD+hypcrtension  and  patients  with IHD+unstable angina.The results revealed  that the mean of platelets (mm')  was  significantly  reduced   in  IHD+unstable  angina  group

2.72.L I.l2 compared with conrol b'TOUp 8.13±2.72.

With respect to the immunological study, the re;;ults revea

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