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Role of Osteopontin Gene Polymorphism and Certain Types of Interleukins in Pathophysiology of Osteoporosis in Iraqi postmenopausal Women
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Osteoporosis (OP) is asymptomatic disease until the experience of falls or impacts that cause broken bones or fractures happened. Estrogen deficiency, abnormality in bone matrix components and interleukins expression can impact on bone homeostasis which gradually paved to osteoclastogenesis over activation leading to bone loss. A current case-control study was designed to explain the alteration in certain biochemicals, bone matrix components, immunological and molecular parameters that which accompanied with OP in the postmenopausal women. The period of study's investigation was from December 2022 to July 2023. All participants provided written informed consent, and the study was approved by Department of Biology, College of Science, University of Baghdad Ethical Council (Reference code CSEC/0922/0092, on 26-9-2022). One hundred and ten postmenopausal women were randomly chosen to participate in this study based on specific criteria from Al-Wasity Hospital, Medical City and Al- Imamein Kadhimein Medical City Hospitles, Baghdad, Iraq. The age of the participants was ranged from 45 to 70 years. Firstly, Dual Energy X-ray Absorptiometry (DEXA) scan was performed to all participants in order to assess the precence or abscense of OP disease. Out of 110 samples, 70 samples were confirmed with OP. Meanwhile, 40 samples were control. Venous blood samples were collected from all participants to estimated compelet blood count (CBC), immunological parameters as C-Reactive Protein (CRP), Rheumatoid Facter (RF), Anticyclical Citrullinated Peptide Antibody (ACPA), Interleukin-8 (IL8), Interleukin (IL-17), and Interleukins-22 (IL-22) using enzyme link immunosorbent assay (ELISA) technique, Parathyroid Hormone (PTH), and Calicum (Ca+2), bone matrix component parameters osteopontin (OPN), osteocalicn (OCN), and osteonectin (ON), in addition to,Osteopontin gene SNP rs11730582 polymorphism was determined by the Hight Resolution Melting (HRM) analysis. Highly significant (P≤0.01) decrease in WBC count (6.30±0.18 k/mcL) and HGB (9.900±0.132 g/dL) were observed in OP patients as when compared with the control group, WBC was (8.26±0.27k/mcL) and HGB was (13.321±0.287 g/dL), Conversely, highly significant (P≤0.01) increase was recorded in ESR (43.73±1.76 mm/hr), PLT (437.88±7.00 1083/uL) in OP patients in compare with control, (21.10±1.25mm/hr), and (226.79±9.39 1083/uL), respectively. However, highly significant (P≤0.01) increase was noticed in immunological parameters: CRP was (18.26±1.33 pg/ml), RF (IU/ml) results were positive, and ACPA was (23.26±2.74EU/ml) in OP patients when compared with control, CRP (2.92±0.18 pg/ml), RF (IU/ml) results were negative, and ACPA was (6.26±0.56 EU/ml). Beside to, highly significant (P≤0.01) increase was recorded in IL-8 serum level (249.08 ±19.98ng/ml), IL-17 (107.862±9.25ng/ml), and IL-22 (57.73±2.47 ng/ml) in OP patients as compared with the healthy control group were IL-8 (65.29 ± 5.62 ng/ml), IL-17 (46.135 ± 8.66 ng/ml) and IL-22 (13.57 ± 3.16 ng/ml). The mean PTH serum level of OP patients group was (77.80±1.79pg/mL) and in control group was (35.81±2.06pg/mL), whereas a highly significant (P≤0.01) decrease in mean of Ca⁺² serum level was recorded in OP patients (4.89±0.21 mmol/L) in compared with control group (9.63±0.13 mmol/L). Additionally, high significant (P≤0.01) increase was detected in OPN serum level (16.88±0.77 ng/ml) and OCN (29.86±2.25ng/ml) in OP patients as compared with the healthy control group (5.95±0.41ng/ml and 10.73±0.86ng/ml), respectively. In contrast, ON serum level recorded a highly significant (P≤0.01) decrease in the patient group (1.78±0.971ng/ml) compared with the control group (3.42±0.20ng/ml). The frequency of homozygote TT genotype in patient's analysis was, (14.3%), (10/70) than control group (27.5%), (11/40). While, the frequency heterozygote TC and homozygote mutant CC genotypes were [TC= (71.42%) 50/70 and CC= 14.3% (10/70)] and these genotypes frequency were not significantly differet from those of the control group, (TC= 60%, (24/40), and (CC= 12.5% 5/40). P-value was different (0.0158) significantly in genotype distribution of rs11730582 polymorphism in OP patients whereas, the P-value of rs11730582 was (0.1500) non-significant in control group. In conclusion, this study found that OPN, OCN, and ON protein act as a sensitive monitoring indicator can be used in early detection of osteoporosis. And because of the capacity to activate osteoclastogenesis and promote bone resorption, IL-8, IL-17, IL-22 and rs11730582 of OPN gene polymorphism may be serve as a biomarkers, pro-inflammatory and immune-stimulating factors in OP development.

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Publication Date
Mon Mar 07 2022
Journal Name
Journal Of Educational And Psychological Researches
Moral identity and its relationship to social affiliation among university students Exposed to shock pressure
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The current research aimed to identify the level of moral identity and social affiliation among students exposed to shock pressures, as well as to reveal the relationship between these variables. To achieve these objectives, the researcher adopted the diagnostic tool for the measure of post-traumatic stress disorder (PDS-5) scale (Foa, 2013) translated to Arabic language by (Imran, 2017). The researcher also adopted the moral identity scale built by (Al-Bayati, 2015) and the measure of social affiliation built by (Al-Jashami, 2013), which were applied to a random sample of (200) male and female students chose from al Anbar University. They were exposed to shock pressures. The results of the research showed that the sample has an average

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Publication Date
Mon Mar 23 2020
Journal Name
Journal Of Engineering
Experimental and Numerical Study on CFRP-Confined Square Concrete Compression Members Subjected to Compressive Loading
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Strengthening of the existing structures is an important task that civil engineers continuously face. Compression members, especially columns, being the most important members of any structure, are the most important members to strengthen if the need ever arise. The method of strengthening compression members by direct wrapping by Carbon Fiber Reinforced Polymer (CFRP) was adopted in this research. Since the concrete material is a heterogeneous and complex in behavior, thus, the behavior of the confined compression members subjected to uniaxial stress is investigated by finite element (FE) models created using Abaqus CAE 2017 software.

The aim of this research is to study experime

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Publication Date
Wed Jun 01 2016
Journal Name
Journal Of Economics And Administrative Sciences
The relationship between Iraq's foreign trade and economic growth analysis For the period (2013-1980)
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Abstract

This study highlights the importance of Iraq in the analysis of foreign trade and economic growth for the period (1980 - 2013) is an attempt to determine the equilibrium relationship long term and short term between these two variables were used ARDL model to explain the economic relationship between the two variables.

To achieve the objectives of the research has been the standard model estimate after testing the stability of exports X data series, and imports M, and GDP current prices, and exchange rate EXR, and verify the existence of a joint integration relationship between these variables.

In order to achieve the objectives of the research it

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Publication Date
Sat Oct 01 2022
Journal Name
Journal Of Composites For Construction
Near-Surface-Mounted CFRP for Strengthening Concavely Curved Soffit RC Beams: Experimental and Analytical Investigation
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Publication Date
Wed Sep 17 2025
Journal Name
Molecular Biology Reports
Antibacterial effect and MexA efflux pump Inhibition by green-synthesized copper nanoparticles from Mentha piperita
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Publication Date
Wed May 10 2023
Journal Name
Biomass Conversion And Biorefinery
Lactic acid-based deep eutectic solvents and activated carbon for soap removal from crude biodiesel
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Publication Date
Wed May 08 2024
Journal Name
Journal Of Mathematics And Computer Science
How does media coverage affect a COVID-19 pandemic model with direct and indirect transmission?
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In this paper, a compartmental differential epidemic model of COVID-19 pandemic transmission is constructed and analyzed that accounts for the effects of media coverage. The model can be categorized into eight distinct divisions: susceptible individuals, exposed individuals, quarantine class, infected individuals, isolated class, infectious material in the environment, media coverage, and recovered individuals. The qualitative analysis of the model indicates that the disease-free equilibrium point is asymptotically stable when the basic reproduction number R0 is less than one. Conversely, the endemic equilibrium is globally asymptotically stable when R0 is bigger than one. In addition, a sensitivity analysis is conducted to determine which

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Publication Date
Wed Aug 01 2018
Journal Name
International Journal Of Engineering
Esterification Reaction Kinetics Using Ion Exchange Resin Catalyst by Pseudo-Homogenous and Eley-Ridel Models
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This work deals with kinetics and chemical equilibrium studies of esterification reaction of ethanol with acetic acid. The esterification reaction was catalyzed by an acidic ion exchange resin (Amberlyst- 15) using a batch stirred tank reactor. The pseudo-homogenous and Eley-Rideal models were successfully fitted with experimental data. At first, Eley-Rideal model was examined for heterogeneous esterification of acetic acid and ethanol. The pseudo-homogenous model was investigated with a power-law model. The apparent reaction order was determined to be (0.88) for Ethanol and (0.92) for acetic acid with a correlation coefficient (R2) of 0.981 and 0.988, respectively. The reaction order was determined to be 4.1087x10-3 L0.8/(mol0.8.min) with

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Publication Date
Sat Nov 01 2025
Journal Name
Construction And Building Materials
Polyalphaolefin as a potential modifying agent for hard asphalt cement: Physical, rheological, and chemical characterization
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Hard-grade asphalt binders like AC20-30 typically exhibit excessive stiffness, reduced penetration, and compromised workability, necessitating modification before use in paving applications. This study evaluates the efficacy of regular polyalphaolefin (PAO), a synthetic olefin-based lubricant, as a performance-enhancing modifying agent for such binders. AC20-30 was blended with PAO at dosages ranging from 2 wt.% to 10 wt.%, and the modified binders were characterized via penetration, ductility, softening point, and rotational viscosity measurements, alongside advanced rheological and chemical-morphological analyses. Incorporating PAO in AC20-30 asphalt progressively reduced the binder stiffness and enhanced its flexibility, with all modifie

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Publication Date
Fri Jun 30 2023
Journal Name
Samarra Journal Of Pure And Applied Science
New Ligand-Based Metal Complexes: Synthesis, Spectroscopic Characterization, Thermal Behavior, Anticancer Properties, and Biological Activity
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New ligands, N1, N4-bis (benzo[d]thiazol-2- ylcarbamothioyl) succinamide (L1) and N1, N4- bis (benzylcarbamothioyl)succinamide (L2), derived from succinyl chloride and 2-amino benzothiazole or benzylamine, respectively, have been used to prepare a set of transition metal complexes with the general formula [M2(L)Cl4], where L=L1 or L2, M = Mn(II), Ni(II), Cu(II), Cd(II), Co(II), Zn(II) or Hg(II). The synthesized compounds were characterized using various analytical techniques including TGA, 13C NMR, mass spectroscopy, 1H and Fourier-transform infrared (FTIR) spectroscopy, magnetic measurement, molar conductivity, electronic spectrum, (%M, %C, %H, %N) and atomic absorption flame (AAF) analysis. The results showed that (L1, L2) bin

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