Osteoporosis (OP) is asymptomatic disease until the experience of falls or impacts that cause broken bones or fractures happened. Estrogen deficiency, abnormality in bone matrix components and interleukins expression can impact on bone homeostasis which gradually paved to osteoclastogenesis over activation leading to bone loss. A current case-control study was designed to explain the alteration in certain biochemicals, bone matrix components, immunological and molecular parameters that which accompanied with OP in the postmenopausal women. The period of study's investigation was from December 2022 to July 2023. All participants provided written informed consent, and the study was approved by Department of Biology, College of Science, University of Baghdad Ethical Council (Reference code CSEC/0922/0092, on 26-9-2022). One hundred and ten postmenopausal women were randomly chosen to participate in this study based on specific criteria from Al-Wasity Hospital, Medical City and Al- Imamein Kadhimein Medical City Hospitles, Baghdad, Iraq. The age of the participants was ranged from 45 to 70 years. Firstly, Dual Energy X-ray Absorptiometry (DEXA) scan was performed to all participants in order to assess the precence or abscense of OP disease. Out of 110 samples, 70 samples were confirmed with OP. Meanwhile, 40 samples were control. Venous blood samples were collected from all participants to estimated compelet blood count (CBC), immunological parameters as C-Reactive Protein (CRP), Rheumatoid Facter (RF), Anticyclical Citrullinated Peptide Antibody (ACPA), Interleukin-8 (IL8), Interleukin (IL-17), and Interleukins-22 (IL-22) using enzyme link immunosorbent assay (ELISA) technique, Parathyroid Hormone (PTH), and Calicum (Ca+2), bone matrix component parameters osteopontin (OPN), osteocalicn (OCN), and osteonectin (ON), in addition to,Osteopontin gene SNP rs11730582 polymorphism was determined by the Hight Resolution Melting (HRM) analysis. Highly significant (P≤0.01) decrease in WBC count (6.30±0.18 k/mcL) and HGB (9.900±0.132 g/dL) were observed in OP patients as when compared with the control group, WBC was (8.26±0.27k/mcL) and HGB was (13.321±0.287 g/dL), Conversely, highly significant (P≤0.01) increase was recorded in ESR (43.73±1.76 mm/hr), PLT (437.88±7.00 1083/uL) in OP patients in compare with control, (21.10±1.25mm/hr), and (226.79±9.39 1083/uL), respectively. However, highly significant (P≤0.01) increase was noticed in immunological parameters: CRP was (18.26±1.33 pg/ml), RF (IU/ml) results were positive, and ACPA was (23.26±2.74EU/ml) in OP patients when compared with control, CRP (2.92±0.18 pg/ml), RF (IU/ml) results were negative, and ACPA was (6.26±0.56 EU/ml). Beside to, highly significant (P≤0.01) increase was recorded in IL-8 serum level (249.08 ±19.98ng/ml), IL-17 (107.862±9.25ng/ml), and IL-22 (57.73±2.47 ng/ml) in OP patients as compared with the healthy control group were IL-8 (65.29 ± 5.62 ng/ml), IL-17 (46.135 ± 8.66 ng/ml) and IL-22 (13.57 ± 3.16 ng/ml). The mean PTH serum level of OP patients group was (77.80±1.79pg/mL) and in control group was (35.81±2.06pg/mL), whereas a highly significant (P≤0.01) decrease in mean of Ca⁺² serum level was recorded in OP patients (4.89±0.21 mmol/L) in compared with control group (9.63±0.13 mmol/L). Additionally, high significant (P≤0.01) increase was detected in OPN serum level (16.88±0.77 ng/ml) and OCN (29.86±2.25ng/ml) in OP patients as compared with the healthy control group (5.95±0.41ng/ml and 10.73±0.86ng/ml), respectively. In contrast, ON serum level recorded a highly significant (P≤0.01) decrease in the patient group (1.78±0.971ng/ml) compared with the control group (3.42±0.20ng/ml). The frequency of homozygote TT genotype in patient's analysis was, (14.3%), (10/70) than control group (27.5%), (11/40). While, the frequency heterozygote TC and homozygote mutant CC genotypes were [TC= (71.42%) 50/70 and CC= 14.3% (10/70)] and these genotypes frequency were not significantly differet from those of the control group, (TC= 60%, (24/40), and (CC= 12.5% 5/40). P-value was different (0.0158) significantly in genotype distribution of rs11730582 polymorphism in OP patients whereas, the P-value of rs11730582 was (0.1500) non-significant in control group. In conclusion, this study found that OPN, OCN, and ON protein act as a sensitive monitoring indicator can be used in early detection of osteoporosis. And because of the capacity to activate osteoclastogenesis and promote bone resorption, IL-8, IL-17, IL-22 and rs11730582 of OPN gene polymorphism may be serve as a biomarkers, pro-inflammatory and immune-stimulating factors in OP development.
Background. Colorectal cancer, ranking second place in global cancer mortality, arises from diverse causes. There is growing recognition of the substantial involvement of the epigenetic modifications of histones at the DNA level in the occurrence of CRC. Aim. To assess the expression of p53, HDAC1, and HDAC3 proteins in a cohort of CRC patients and to analyze potential relationship between their expression and the stages of CRC progression. Materials and Methods. The retrospective investigation was carried out on 95 paraffin-embedded CRC tissue samples. The expression of p53, HDAC1, and HDAC3 was assessed immunohistochemically. Results. Notably, the expression of the p53 protein in CRC tissue samples exhibited a prominent correlatio
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Study the role of CoQ10 and IGFBP-1 in obese male patients with diabetic mellitus type 2. ELISA method was used to assay Serum CoQ10 and IGFBP-1. Blood was taken with drawn sample from 30 obese normal patients with age range (40-60) years, 30 diabetic patients with age range (40-60) years at duration of disease (1-5) years and 30 normal healthy patients. The mean difference between T2DM according to CoQ10 (12.5±1.1) was decreased than the mean of IFG (21.8±3.2) (P 0.002) and the mean difference between T2DM according to IGFBPs (0.65±0.06) was decreased than the mean of IFG (3.2±0.3) (P 0.000). While no significant difference between mean age of DM2 patients (55.5±1.06), and IFG (55.6±0.9) (p 0.90), no significant difference bet
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Construction is the opening of the important pillars of the construction of the film as a whole for this, we find that the first of any narrative of my film begin at the borders of this construction is the window that we look through the contents tale and puzzle narrative is of significance that degrade traveler when reservoirs expression later in reasoning and find justifications ills that came by those initiation, this initiation may be the window that lead us to the core, understanding the story through signals received to the recipient to sail because of the paths of pickling what is which is encoded, but this initiation may serve as keys that understanding the be puppies and signals that beset and surrounded to what He holds inevita
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The first chapter includes introduce the research and the problem of the research that there are negligence of some educational institutions and schools management , some parents and supervisors .Also there are some educators who believes that school activities are obstacles means to learning and prevent pupils from studying and understanding the subjects. That may due to social , psychological and educational reasons
The purposes of the researches come from the reports of supervision visits which indicate a lot of difficulties faced by pupils and teachers related to education activities and its influence on in education and the necessity of indentify these difficulties and remedy them
... Show MoreCorruption, in all its categories and forms, is regarded as the nowadays virus which has greatly spread in most institutes and society, a matter that cause a great waste of resources.
According to the reports of international transparency Institute, Iraq is regarded as one of the greatest countries in corruption.
Regardless of the reasons and forms of corruption, the retreat in work – values and ethics are the main reasons behind that.
Being the main source of providing qualified staff "educators" for the working market, the high education institutes face great challenges in standing against corruption inside and outside
... Show MoreAbstract: In recent times, global attention has increasingly focused on the critical issue of environmental sustainability, owing to escalating environmental degradation exacerbated by the utilization of green spaces and technological innovation. This phenomenon necessitates thorough examination, prompting the present study to scrutinize the impact of various factors, namely green spaces, technological innovation, environmental taxes, renewable energy consumption (REC), inflation, and economic growth (EG), on environmental sustainability within the context of Iraq. Secondary data extracted from the World Development Indicators (WDI) spanning the period from 1991 to 2022 served as the foundation for this investigation. Methodologically, the
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The principal objective of this study is to demonstrate how green quality management and product life cycle costing may help an organization gain a competitive advantage. Green quality management's influence on increasing product quality and meeting environmental criteria, as well as tracking the activities of product life cycle before, during, and after production, is demonstrated. Orienting these activities toward the production of eco-friendly products that fulfill the needs of customers, hence increasing organization's market share. We found from our study that proposed framework can help organizations improve their competitiveness. Green quality management contributes to environmental protection and the provision of high-qualit
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The aggregation capacity of human reb blood cells lies between that of the non- aggregated arythrocyte and the remarkably full sedimentation. As the ability to aggregate is atributed to many factors such as the availability of macromolecules and plasma lipids, the role of plasm lipid profile on RBC aggregation and sedimentation changes in normal and diabetic patients is studied.Also serum lipid profile measurement (Total cholesterol, Triglyceride, HDL, LDL, VLDL) in normal and diabetic subjects were made. The principle of measurement includes detecting the transmitted laser light through a suspension of 10% diluted red blood cells in plasma. In all diabetics, the raulux formation and sedimentation rate is enhanced.
The study seeks to analyze the perceptions of audience in UAE towards the performance of Emirates Satellite TV Channels. It analyzed the exposure motivations of audience to satellite TV channels, its positive and negative aspects and to what extent they abide by media ethics. A survey is conducted with a sample of four hundred. The study shows significant differences between male and female towards the characteristics of TV channels, its positive and negative aspects and its commitments to media ethics.
The study also shows that the expectancy value model and third person effect model are applicable in studying the perceptions of audience and media people in UAE t
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Since the emergence of the science of international relations as an independent academic scientific field, various theories and trends have appeared and have tried to understand and explain the international reality and give a clear picture of what is happening within the international system of interactions and influences and the search for tools for stability and peace in international relations. Among these theories is the feminist theory, which is a new intellectual trend on the level of international relations theories, which tried to give an explanation of what is happening in world politics and in international relations in particular. The main issue that feminist theory is concerned with is the lack of women’s subordination
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