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ShcejJIBVTCNdQwCfbUJ
Chromogenic in Situ Hybridization Technique versus Immunohistochemistry in Assessment of HER2/neu Status in 448 Iraqi Patients with Invasive Breast Carcinoma
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BACKGROUND: The rapidly growing knowledge regarding factors controlling tumour growth, with the new modalities of therapy acting on the biological activity of the tumours draw the attention of most cancer researches nowadays and represent a major focus for clinical oncology practice. For the detection of HER2/neu protein overexpression and gene amplification, immunohistochemistry (IHC) and in-situ hybridisation (ISH) is the recommended techniques, respectively, with high concordance between the two techniques. The current United Kingdom recommendations for HER2/neu testing are either for a two-tier system using IHC with reflex ISH testing in equivocal positive cases, or a one-tier ISH strategy. AIM: To compare the results of HER2/neu gene status in patients with breast carcinoma obtained by chromogenic in situ hybridisation with those obtained by immunohistochemistry, and to compare these results with hormonal receptors expression by immunohistochemistry and with age of patients.METHODS: Immunohistochemistry technique was used for evaluation of status of estrogen receptors (ER) and progesterone receptors (PR) and HER2/neu protein expression in 448 Iraqi patients with invasive breast carcinoma with different grades and histological types and then chromogenic in situ hybridization (CISH) technique was applied for all scores of HER2/neu to detect the gene status and compare the results in all negative, equivocal and positive cases by immunohistochemistry (IHC). The cases were referred from different centres, and IHC and CISH techniques were done in central public health laboratory in Baghdad over 28 months, from July 2013 to November 2015. A comparison of the results was made to find the relationship between HER2/neu and hormone receptors status and other clinical parameters like patients age. RESULTS: The mean age of the study cases was 49.08 years, ranging from 24 to 83 years. Of the 448 cases of breast carcinoma, 44 (9.8%) cases were of score 0 by IHC, none of them (0%) showed HER2/neu gene amplification by CISH. 71(15.8%) cases were of score 1 by IHC, 15 (21.12%) of them showed HER2/neu gene amplification by CISH, all were of low amplification. There were 306 (68.3%) cases of score 2 by IHC, of which 102 (33.33%) cases showed HER2/neu gene amplification by CISH, with 79 (25.81%) of them with low amplification and 23 (7.51%) cases with high amplification, while only one case (0.32%) remained in equivocal category. In score 3, all the 27 (6.0%) cases showed gene amplification with 12 (44.44%) cases with low amplification and 15 (55.55) cases with high amplification with overall percentage of gene amplification in score 3 of 100%. There was a significant inverse relationship between hormone receptors (ER and PR) status and HER2/neu gene amplification. No significant relationship was found between the patient’s age and HER2/neu gene amplification.CONCLUSION: Although immunohistochemistry is a widely used, less expensive and reliable test, we strongly advice performance of chromogenic in situ hybridization in assessment of HER2/neu gene status in all cases diagnosed with breast carcinoma as significant number of cases that were reported as negative by immunohistochemistry showed positive amplification by chromogenic in situ hybridization and can get benefit from anti-HER2 targeted treatments.

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Publication Date
Wed Oct 06 2021
Journal Name
College Of Islamic Sciences
antonyms in Quranic readings
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This research aims to investigate the impacts of Quranic readings on the meanings of antonyms in the Quran, with considering the practical aspects that clarify them. The importance of this topic lies in clarifying the importance of the science of Qiraʼat, its great value and its high status. Moreover, this research sheds light on the relationship between Quranic readings and the antonyms words, following the descriptive approach in the study. The research has found several findings, the most prominent of which was: antonyms are linguistic phenomena that are proven to occur in Quranic readings, so each reading deliver a meaning that is opposite to the meaning learned from the other reading.In addition, Quranic readings are considered as

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Publication Date
Thu Sep 15 2022
Journal Name
Aladab Journal
Adjectives in Shakespeare's Sonnets
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The present paper studies the use of adjectives in Shakespeare's Sonnets (4, 5, 6, & 9), which are chosen randomly, on the three levels: grammar, lexis and conversation. It tackles adjectives as a literary term with presenting their forms and functions. The presentation is done according to the themes, their forms and functions in conversation, the adjectives are shown in this paper as for their forms, frequency and meanings in the sonnets and their effects on studying. The analysis is done according to Quirk et al. (1985) and Quirk and Greenbaum (1973). Then, all adjectives have been calculated in the sonnets. The results of the analysis have shown that compound adjectives which occurred (31) are used more than the Suffixation

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Publication Date
Sun Jul 01 2018
Journal Name
Journal Of Educational And Psychological Researches
Equality in Islamic Thought
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The concept of equality in Islamic thought emanates from the unity of human entity that does not breach the concept of equality in itself and it should take differentiation among people as means for development and growth not as excuse for injustice and discrimination. Islamic thought has left all the prevailing norms of differentiation such as (weakness and strength, economic and social position, gender, color, and social class). Islamic thought has underscored the quality of people of different race, race, color, and language which was not familiar in those civilizations before the emergence of Islam such as (Egyptian, Persian, roman civilization). It was common to divide people into different social classes. The aim of Islam is to kee

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Publication Date
Fri Aug 13 2021
Journal Name
Male Reproductive Anatomy
Epigenetics in Male Infertility
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Publication Date
Wed Jan 15 2020
Journal Name
Arab World English Journal
Posters in Vocabulary Learning
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An essential element in English as a foreign language (EFL) learning is vocabulary. There is a big emphasis on learning the new words' meaning from the books or inside classrooms. Also, it is a major part of language teaching as well as being fundamental to the learner but there is a big challenge in vocabulary instruction due to the weak confidence by teachers in selecting the suitable practice in teaching vocabulary or they sometimes unable to specify a suitable time for it during the teaching process. The major aim of this study is to investigate the value of posters in vocabulary learning on the 2nd grade students at Halemat Alsaadia High School in Baghdad – Iraq. It hypothesized that there are no statistically significant differences

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Publication Date
Sun Dec 09 2018
Journal Name
Baghdad Science Journal
Tadpole Domination in Graphs
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A new type of the connected domination parameters called tadpole domination number of a graph is introduced. Tadpole domination number for some standard graphs is determined, and some bounds for this number are obtained. Additionally, a new graph, finite, simple, undirected and connected, is introduced named weaver graph. Tadpole domination is calculated for this graph with other families of graphs.

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Publication Date
Sun Sep 01 2013
Journal Name
Iosr Journal Of Applied Chemistry (iosr-jac)
Neonatal Omphalitis in Iraq
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Publication Date
Sat Feb 09 2019
Journal Name
Journal Of The College Of Education For Women
Excellence in Language Education
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The primary goal of the study is to practise some systematic strategies for foreign language instruction despite the fact that no single approach to language classroom instruction is inherently superior to others; instead the choice of an approach should be determined by the teacher, students, the subject matter, and the situation. The paper adopts certain strategies which may assist EFL teachers to excel in their work and continue their professional development. Hence, it is intended for language teachers who understand the value of good teaching and like to produce academically successful students. The study could also help college student teachers to acquire new strategies in order not only to succeed but also to excel in their learni

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Publication Date
Sat Feb 09 2019
Journal Name
Journal Of The College Of Education For Women
Extravagant in the Koran
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This research deals with a disease of the diseases our Muslim society, which works in the
body operate deadly epidemics in the bodies and of bodies, but it is extravagant; has spread
and rampant in our uncle everything which threatens significant damage in this life and the
afterlife, as well as that it shows that Alasirafla limited to extravagant in wasting money and
with this blessing should not have, and not be used as created him .
Excessive linked to all aspects of life, physical and moral it has many pictures and various
forms, came at the forefront of research and five sections:
The first topic: the meaning of truth and extravagant.
The second topic: Photos extravagant reprehensible contained in the Koran.<

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Publication Date
Sat Jun 06 2020
Journal Name
Journal Of The College Of Education For Women
Psychological Intentions in Hadiths
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The present study aims to shed light on psychological intentions in some prophetic hadiths and present practical models and realistic applications that reveal the aim behind Prophet Mohammed’s actions and speech. This study relied on a descriptive and deductive approach. It included three demands. The first demand provided a glimpse of the theoretical and conceptual framework of psychological intentions and their most important classifications. The second request tackled the history of Makassed thought in the Prophet’s hadiths. The third requirement dealt with the presentation of the physical, mental, and psychological intentions in Sunnah. The results revealed that The Islamic religion seeks to achieve happiness for the human being

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