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Molecular detection and the frequency of a pore-forming toxin in Enterococcus faecalis isolated from urinary tract infections
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Background: Enterococcus faecalis is a causative agent for urinary tract infections (UTIs) in Iraq and worldwide, even though it is a commensal bacterium in human and animal intestines. It can cause different illnesses due to its ability to produce various virulence factors. A pore-forming toxin (cytolysin) is the most virulence factor in this bacterium. Objective: This study aims to molecularly investigate the frequency of cytolysin toxin among E. faecalis isolated from UTIs. Methods: A hundred and eighty urine specimens were collected from females diagnosed with UTIs. Traditional laboratory and molecular methods were used for bacterial identification and toxin detection using a modified DNA extraction method. Results: The findings revealed that 27.7% (50\180) of causative agents in UTIs were E. faecalis based on the molecular technique that targeted a housekeeping gene (ddI) with specific primers using polymerase chain reaction (PCR). Most of the isolates harboured the cytolysin toxin gene (cylLL) with a frequency rate of 92% (46\50). Conclusions: A considerable prevalence of cytolysin-positive isolates in UTIs, which is a worrying indicates of the extensive spreading of a toxic strain in UTIs. The modified method for DNA extraction in gene detection was successfully used to amplify a housekeeping gene (ddI) and a virulence gene (cylLL) for cytolysin toxin detection, and this approach can be utilised for rapid bacterial identification and gene detection in medical and research purposes with a large sample size in an inexpensive manner within a short time.

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Publication Date
Mon May 28 2018
Journal Name
Iraqi Journal Of Science
Construction of a Robust Background Model for Moving Object Detection in Video Sequence
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Background Subtraction (BGS) is one of the main techniques used for moving object detection which further utilized in video analysis, especially in video surveillance systems. Practically, acquiring a robust background (reference) image is a real challenge due to the dynamic change in the scene. Hence, a key point to BGS is background modeling, in which a model is built and repeatedly used to reconstruct the background image.

From N frames the proposed method store N pixels at location(x,y) in a buffer, then it classify pixel intensity values at that buffer using a proposed online clustering model based on the idea of relative  run length, the cluster center with the highest frequency will be adopted as the background pixel

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Publication Date
Fri Jun 02 2017
Journal Name
International Journal Of Medical Research & Health Sciences
Analysis of HLA-DQB1 Alleles Frequency in Patients with Chronic Periodontitis
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Publication Date
Fri Sep 17 2021
Journal Name
Journal Of Petroleum Exploration And Production Technology
Characterization of flow units, rock and pore types for Mishrif Reservoir in West Qurna oilfield, Southern Iraq by using lithofacies data
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Abstract<p>This study has been accomplished by testing three different models to determine rocks type, pore throat radius, and flow units for Mishrif Formation in West Qurna oilfield in Southern Iraq based on Mishrif full diameter cores from 20 wells. The three models that were used in this study were Lucia rocks type classification, Winland plot was utilized to determine the pore throat radius depending on the mercury injection test (r35), and (FZI) concepts to identify flow units which enabled us to recognize the differences between Mishrif units in these three categories. The study of pore characteristics is very significant in reservoir evaluation. It controls the storage mechanism and reservoir fluid prope</p> ... Show More
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Publication Date
Thu Jun 01 2023
Journal Name
Online Journal Of Veterinary Research
Article review: Infections by gram + bacteria in laboratory mice
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Publication Date
Wed Feb 08 2023
Journal Name
Iraqi Journal Of Science
Uropathogenic Infections Among Patients with Diabetes in Baghdad City
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One hundred thirty four urine samples were collected from diabetic patients during the period from March –November -2014. From these samples there were 62.7% positive samples, women represented 45.3% and men represented 54.7% , some of them were a symptomatic as women represented 60.5% and men represented 58.7%  and some were with clinical symptoms with or without a history of UTI. Hypertension diabetic women represented 45% and men represented 26% .Men suffering from prostate enlargement were represented 35% from the total diabetics .The E.coli isolates were  represented 28.6% from the total cases ,Klebsiella spp  isolates represented 20%, S. aureus   isolates represented 16.7%,P

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Publication Date
Wed Mar 10 2021
Journal Name
Baghdad Science Journal
Morphological, Molecular and Pollen Grain Investigations of Salix Species in Egypt
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Genus Salix is among family Salicaceae, distributing in the northern hemisphere. It is represented in Egypt by two species (Salix mucronata and Salix tetrasperma). The classification of Salix at the generic and infra-generic levels is still outstanding. We have agreed to list the Egyptian species of this genus. We collected them during field trips to most Egyptian habitats; fresh and herbarium specimens were subjected to taxonomic revision based on morphological characters; scanning electron microscope (SEM) for pollen grains; isozyme analysis using esterase and peroxidase enzymes and genetic diversity using random amplified polymorphic DNA (RAPD). We recorded that both sexes of S.

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Publication Date
Fri Jan 26 2024
Journal Name
Iraqi Journal Of Science
Antimicrobial Effects of Black Tea (Camellia sinensis) on Pseudomonas Aeruginosa Isolated from Eye Infection
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This study was conducted to evaluate the effects of black tea on Pseudomonas
aeruginosa isolated from eye infection. One hundred samples (corneal scrapings)
were obtained. Approximately, 77% of the cases were due to contact lens wear
followed by 15 % trauma and 8% with unknown history. The isolates identified as
P. aeruginosa were 30% (23/77 CL) and 25% (2/8 Unknown). On the other hand,
the Kirby-Bauer antibiotic sensitivity assay showed that 100% of the isolates were
sensitive to Neomycin, Gentamicin and Amikacin. While 91.6% were sensitive to
Carbenicillin and Ceftriaxone; 66.6% were sensitive to Cefotaxime and 0% were
sensitive to Tertacycline. Only two isolates were found to be multidrug resistant.
Screenin

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Publication Date
Mon Jan 28 2019
Journal Name
Iraqi Journal Of Science
Synergistic Effect of Linezolid, Tigecycline, and Vancomycin on Staphylococcus Aureus Isolated From Iraqi Patients with Diabetic Foot Ulcers
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Ninety nine swabs were collected from patients with diabetic foot ulcers (DFU), all swabs   were cultured on different selective media for screening, 46 isolates confirmed as S. aureus by API staph. The results of antibiotic susceptibility test revealed that all isolates were resistant to metronidazole, 34 isolates were resistant to cefoxitin, ceftriaxone, and meropenim, 23 isolates were resistant to ciprofloxacin and norfloxacin, 17 and 16 isolates were resistant to tetracycline and trimethoprim, respectively; while all isolates were sensitive to tigecycline. The results of minimum inhibitory concentration (MIC) that carried out by using vancomycin, tigecycline and linezolid for 8 isolates, MIC results were1-2 µg /ml

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Publication Date
Fri Aug 31 2012
Journal Name
Al-khwarizmi Engineering Journal
Sub–Nyquist Frequency Efficient Audio Compression
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This paper presents the application of a framework of fast and efficient compressive sampling based on the concept of random sampling of sparse Audio signal. It provides four important features. (i) It is universal with a variety of sparse signals. (ii) The number of measurements required for exact reconstruction is nearly optimal and much less then the sampling frequency and below the Nyquist frequency. (iii) It has very low complexity and fast computation. (iv) It is developed on the provable mathematical model from which we are able to quantify trade-offs among streaming capability, computation/memory requirement and quality of reconstruction of the audio signal. Compressed sensing CS is an attractive compression scheme due to its uni

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Publication Date
Tue Mar 15 2022
Journal Name
Journal Of Baghdad College Of Dentistry
In vitro cytotoxic effect of annona squamosa pulp ex-tract as a mouthwash for children on human normal cell line
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Background: Fruits and their by-products are the primary sources of bioactive chemicals in plants. Because of its phytochemical richness, Annona squamosa fruits have gained the alertness of people willing in health-promoting diets. The purpose of this in vitro study was to evaluate the cytocompatibility effect of ethanolic crude extract of Annona squamosa pulp against a human normal cell line as a mouthwash for children. Material and method: The ethanolic extract of Annona squamosa pulp was extracted using the ultrasonic method and then lyophilized to make it powder. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) test was performed to investigate the cytotoxic activity of the pulp extract on a human normal cell l

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